Restriction map for
pFN21AE4270
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccI AclI AcyI AflII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BbrPI BciVI
BclI BcnI BfrI BlnI BsaHI
BsiEI BsiWI BsmBI BsmI BspDI
BspHI BssHII Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HpaI
KasI KspI MluI NarI NciI
NcoI NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspI StuI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1728 GGTACC G^GTACC
AccII 1526 CGCG CG^CG
AccIII 2361 TCCGGA T^CCGGA
AhdI 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1728 GGTACC G^GTACC
AspEI 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2098 GCAGTACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 460 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 336 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 336 GCTCAGC GC^T[ACGT]AGC
BsaAI 2948 TACGTG [CT]AC^GT[GA]
BsaBI 1918 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2361 TCCGGA T^CCGGA
BspEI 2361 TCCGGA T^CCGGA
BspLU11I 1250 ACATGT A^CATGT
BsrBI 334 CCGCTC CCGCTC(-3/-3)
BsrFI 2216 GCCGGC [GA]^CCGG[CT]
BstBI 74 TTCGAA TT^CGAA
BstEII 768 GGTCACC G^GT[ACGT]ACC
BstPI 768 GGTCACC G^GT[ACGT]ACC
BstUI 1526 CGCG CG^CG
CelII 336 GCTCAGC GC^T[ACGT]AGC
CfoI 1353 GCGC GCG^C
Cfr10I 2216 GCCGGC [GA]^CCGG[CT]
DraI 609 TTTAAA TTT^AAA
Eam1105I 901 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 768 GGTCACC G^GT[ACGT]ACC
FauI 1865 CCCGC CCCGC(4/6)
HaeII 1352 AGCGCC [GA]GCGC^[CT]
HhaI 1353 GCGC GCG^C
HinP1I 1353 GCGC G^CGC
KpnI 1728 GGTACC GGTAC^C
MamI 1918 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2361 TCCGGA T^CCGGA
MvnI 1526 CGCG CG^CG
NaeI 2216 GCCGGC GCC^GGC
NgoMIV 2216 GCCGGC G^CCGGC
NspV 74 TTCGAA TT^CGAA
PflMI 542 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 2570 GAAGAGC GCTCTTC(1/4)
SexAI 3062 ACCAGGT A^CC[AT]GGT
SfuI 74 TTCGAA TT^CGAA
SpeI 432 ACTAGT A^CTAGT
Van91I 542 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2250 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 36 enzymes) #
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AflIII 1250 ACATGT A^C[GA][CT]GT
1944 ACGTGT
AocI 2701 CCTCAGG CC^T[ACGT]AGG
3313 CCTGAGG
AvaI 1733 CTCGAG C^[CT]CG[GA]G
1787 CTCGAG
BalI 99 TGGCCA TGG^CCA
2852 TGGCCA
BamHI 1600 GGATCC G^GATCC
1782 GGATCC
BbvCI 526 CCTCAGC CCTCAGC(-5/-2)
3199 GCTGAGG
BglII 1689 AGATCT A^GATCT
2559 AGATCT
BsaWI 897 ACCGGA [AT]^CCGG[AT]
2361 TCCGGA
BsoBI 1733 CTCGAG C^[CT]CG[GA]G
1787 CTCGAG
Bsp1407I 6 TGTACA T^GTACA
1300 TGTACA
BsrDI 206 CATTGC GCAATG(2/0)
592 GCAATG
BsrGI 6 TGTACA T^GTACA
1300 TGTACA
BssSI 217 CTCGTG CACGAG(-5/-1)
1286 CTCGTG
BstAPI 113 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
140 GCAAGGCTTGC
Bsu36I 2701 CCTCAGG CC^T[ACGT]AGG
3313 CCTGAGG
DsaI 694 CCACGG C^C[GA][CT]GG
2757 CCACGG
Eco81I 2701 CCTCAGG CC^T[ACGT]AGG
3313 CCTGAGG
EcoNI 3300 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3313 CCTGAGGGAGG
EcoT14I 582 CCTTGG C^C[AT][AT]GG
1675 CCAAGG
HincII 428 GTCAAC GT[CT]^[GA]AC
1391 GTCAAC
HindII 428 GTCAAC GT[CT]^[GA]AC
1391 GTCAAC
HindIII 406 AAGCTT A^AGCTT
605 AAGCTT
MfeI 1013 CAATTG C^AATTG
2914 CAATTG
MluNI 99 TGGCCA TGG^CCA
2852 TGGCCA
MscI 99 TGGCCA TGG^CCA
2852 TGGCCA
MunI 1013 CAATTG C^AATTG
2914 CAATTG
NdeI 946 CATATG CA^TATG
2747 CATATG
NspI 729 GCATGT [GA]CATG^[CT]
1250 ACATGT
PaeR7I 1733 CTCGAG C^TCGAG
1787 CTCGAG
PshAI 414 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1090 GACCCTAGTC
PstI 211 CTGCAG CTGCA^G
2093 CTGCAG
SfcI 211 CTGCAG C^T[GA][CT]AG
2093 CTGCAG
SspBI 6 TGTACA T^GTACA
1300 TGTACA
StyI 582 CCTTGG C^C[AT][AT]GG
1675 CCAAGG
XcmI 2486 CCAAAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3007 CCACCTCCAAAATGG
XhoI 1733 CTCGAG C^TCGAG
1787 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI