Restriction map for
pFN21AB8549
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbrPI BbsI BclI
BfrI BlnI BpuAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
KspI MamI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TaqI TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 449 GGTACC G^GTACC
AflIII 391 ACATGT A^C[GA][CT]GT
Alw44I 1216 GTGCAC G^TGCAC
ApaLI 1216 GTGCAC G^TGCAC
Asp718I 449 GGTACC G^GTACC
AspI 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2882 CCCGGG C^[CT]CG[GA]G
BalI 725 TGGCCA TGG^CCA
BamHI 635 GGATCC G^GATCC
BbeI 2261 GGCGCC GGCGC^C
BbvCI 2122 CCTCAGC CCTCAGC(-5/-2)
BciVI 168 GGATAC GTATCC(6/5)
BglII 720 AGATCT A^GATCT
BsaAI 2225 TACGTG [CT]AC^GT[GA]
BsgI 2840 CTGCAC GTGCAG(16/14)
BsoBI 2882 CCCGGG C^[CT]CG[GA]G
BspHI 279 TCATGA T^CATGA
BspLU11I 391 ACATGT A^CATGT
BssHII 1522 GCGCGC G^CGCGC
BssSI 1900 CTCGTG CACGAG(-5/-1)
EagI 1493 CGGCCG C^GGCCG
EclXI 1493 CGGCCG C^GGCCG
Eco52I 1493 CGGCCG C^GGCCG
HgaI 844 GACGC GACGC(5/10)
HincII 828 GTTAAC GT[CT]^[GA]AC
HindII 828 GTTAAC GT[CT]^[GA]AC
HindIII 922 AAGCTT A^AGCTT
HpaI 828 GTTAAC GTT^AAC
KasI 2261 GGCGCC G^GCGCC
KpnI 449 GGTACC GGTAC^C
MluNI 725 TGGCCA TGG^CCA
MscI 725 TGGCCA TGG^CCA
MslI 255 CACTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2261 GGCGCC GG^CGCC
NheI 459 GCTAGC G^CTAGC
PflFI 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 390 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 279 TCATGA T^CATGA
SapI 1764 GAAGAGC GCTCTTC(1/4)
SbfI 2364 CCTGCAGG CCTGCA^GG
SfiI 1944 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2261 GGCGCC GGC^GCC
SmaI 2882 CCCGGG CCC^GGG
Sse8387I 2364 CCTGCAGG CCTGCA^GG
Tth111I 289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 357 CCAACAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2882 CCCGGG C^CCGGG
XmaI 2882 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 1523 CGCG CG^CG
1573 CGCG
AcyI 844 GACGCC G[GA]^CG[CT]C
2261 GGCGCC
BcgI 1669 GCACTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2856 GCAGCTGCTTCG
BsaHI 844 GACGCC G[GA]^CG[CT]C
2261 GGCGCC
BsmBI 842 GAGACG CGTCTC(1/5)
1270 GAGACG
BsmI 574 GAATGC GAATGC(1/-1)
2915 GCATTC
BsrDI 2502 CATTGC GCAATG(2/0)
881 GCAATG
BstUI 1523 CGCG CG^CG
1573 CGCG
BstXI 2466 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2727 CCAGGAACTTGG
EarI 916 GAAGAG CTCTTC(1/4)
1764 GAAGAG
Hin1I 844 GACGCC G[GA]^CG[CT]C
2261 GGCGCC
Ksp632I 916 GAAGAG CTCTTC(1/4)
1764 GAAGAG
MaeII 1688 ACGT A^CGT
2226 ACGT
MseI 829 TTAA T^TAA
962 TTAA
MvnI 1523 CGCG CG^CG
1573 CGCG
NaeI 2258 GCCGGC GCC^GGC
2779 GCCGGC
NgoMIV 2258 GCCGGC G^CCGGC
2779 GCCGGC
PflMI 1803 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1896 CCAGCTCGTGG
SmlI 1440 CTTGAG C^T[CT][GA]AG
1597 CTCAAG
SphI 687 GCATGC GCATG^C
953 GCATGC
Tru9I 829 TTAA T^TAA
962 TTAA
Tsp45I 937 GTGAC ^GT[GC]AC
2828 GTGAC
Van91I 1803 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1896 CCAGCTCGTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaII BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp509I TspRI XhoII