Restriction map for
pFN21AB5767
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccIII AclI AfaI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BbrPI BbvCI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI ScaI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I XbaI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 88 GTCTAC GT^[AC][GT]AC
AflIII 923 ACATGT A^C[GA][CT]GT
Asp700I 1887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2427 GAGCAC G[AT]GC[AT]^C
AvrII 1221 CCTAGG C^CTAGG
BalI 494 TGGCCA TGG^CCA
BbsI 1405 GTCTTC GAAGAC(2/6)
BlnI 1221 CCTAGG C^CTAGG
BpuAI 1405 GTCTTC GAAGAC(2/6)
BsaI 913 GAGACC GGTCTC(1/5)
BsgI 649 CTGCAC GTGCAG(16/14)
BsiHKAI 2427 GAGCAC G[AT]GC[AT]^C
BspLU11I 923 ACATGT A^CATGT
BsrBI 328 GAGCGG CCGCTC(-3/-3)
BstAPI 1518 GCATGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2295 GGTGACC G^GT[ACGT]ACC
BstPI 2295 GGTGACC G^GT[ACGT]ACC
DraIII 997 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1143 GACTATTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 171 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2295 GGTGACC G^GT[ACGT]ACC
EcoRI 1162 GAATTC G^AATTC
HincII 2152 GTCAAC GT[CT]^[GA]AC
HindII 2152 GTCAAC GT[CT]^[GA]AC
KspI 34 CCGCGG CCGC^GG
MluNI 494 TGGCCA TGG^CCA
MscI 494 TGGCCA TGG^CCA
MslI 208 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1381 CATATG CA^TATG
SacII 34 CCGCGG CCGC^GG
SapI 681 GAAGAGC GCTCTTC(1/4)
SexAI 998 ACCAGGT A^CC[AT]GGT
SfiI 1985 GGCCTGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 947 CCCGGG CCC^GGG
StuI 1644 AGGCCT AGG^CCT
XmaCI 947 CCCGGG C^CCGGG
XmaI 947 CCCGGG C^CCGGG
XmnI 1887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AatII 85 GACGTC GACGT^C
586 GACGTC
AspI 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
483 GACAGCGTC
BbeI 1012 GGCGCC GGCGC^C
1387 GGCGCC
BcgI 540 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
825 GCAGCTCCCTCG
BciVI 1533 GGATAC GTATCC(6/5)
2283 GGATAC
BglI 1986 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2176 GCCACACTGGC
BsmI 381 GCATTC GAATGC(1/-1)
2369 GCATTC
KasI 1012 GGCGCC G^GCGCC
1387 GGCGCC
NarI 1012 GGCGCC GG^CGCC
1387 GGCGCC
NcoI 1193 CCATGG C^CATGG
2259 CCATGG
NspI 923 ACATGT [GA]CATG^[CT]
2156 ACATGC
PflFI 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
483 GACAGCGTC
PvuII 850 CAGCTG CAG^CTG
1916 CAGCTG
SbfI 291 CCTGCAGG CCTGCA^GG
2229 CCTGCAGG
SfoI 1012 GGCGCC GGC^GCC
1387 GGCGCC
Sse8387I 291 CCTGCAGG CCTGCA^GG
2229 CCTGCAGG
Tth111I 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
483 GACAGCGTC
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
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AccII AciI AcsI AcyI AluI
AlwI AlwNI AocI ApaI ApoI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII