Restriction map for
pFN21AE3212
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbvCI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 944 ACACGT A^C[GA][CT]GT
Asp700I 609 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 696 TGGCCA TGG^CCA
BamHI 735 GGATCC G^GATCC
BanI 332 GGTGCC G^G[CT][GA]CC
BbrPI 945 CACGTG CAC^GTG
BcgI 1155 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 820 GGATAC GTATCC(6/5)
BfaI 23 CTAG C^TAG
BglII 1013 AGATCT A^GATCT
BsaWI 377 ACCGGA [AT]^CCGG[AT]
BsmI 1407 GCATTC GAATGC(1/-1)
BspDI 281 ATCGAT AT^CGAT
BspHI 1316 TCATGA T^CATGA
BstXI 593 CCAGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 281 ATCGAT AT^CGAT
DdeI 1331 CTGAG C^T[ACGT]AG
DraII 779 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 513 CCATGG C^C[GA][CT]GG
EarI 490 GAAGAG CTCTTC(1/4)
EcoO109I 779 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 513 CCATGG C^C[AT][AT]GG
Ksp632I 490 GAAGAG CTCTTC(1/4)
MaeI 23 CTAG C^TAG
MfeI 1234 CAATTG C^AATTG
MluNI 696 TGGCCA TGG^CCA
MscI 696 TGGCCA TGG^CCA
MunI 1234 CAATTG C^AATTG
NcoI 513 CCATGG C^CATGG
NspI 401 GCATGC [GA]CATG^[CT]
PflMI 497 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 945 CACGTG CAC^GTG
PmlI 945 CACGTG CAC^GTG
PpuMI 779 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1316 TCATGA T^CATGA
SfaNI 284 GATGC GCATC(5/9)
SfcI 200 CTACAG C^T[GA][CT]AG
SmlI 954 CTTGAG C^T[CT][GA]AG
SphI 401 GCATGC GCATG^C
StyI 513 CCATGG C^C[AT][AT]GG
Van91I 497 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 528 CCACAGTTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 609 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 925 GAGCTC G[AT]GC[AT]^C
957 GAGCTC
AvaII 192 GGACC G^G[AT]CC
780 GGACC
BbsI 588 GTCTTC GAAGAC(2/6)
987 GTCTTC
BpuAI 588 GTCTTC GAAGAC(2/6)
987 GTCTTC
BsaAI 945 CACGTG [CT]AC^GT[GA]
963 TACGTG
BseRI 1387 CTCCTC GAGGAG(10/8)
1156 GAGGAG
BsiHKAI 925 GAGCTC G[AT]GC[AT]^C
957 GAGCTC
BsmAI 179 GTCTC GTCTC(1/5)
312 GTCTC
BsmFI 8 GGGAC GGGAC(10/14)
191 GGGAC
BsrBI 4 GAGCGG CCGCTC(-3/-3)
1105 GAGCGG
BstYI 735 GGATCC [GA]^GATC[CT]
1013 AGATCT
EaeI 696 TGGCCA [CT]^GGCC[GA]
866 TGGCCG
EcoNI 22 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1389 CCTCTTTAAGG
EcoRV 441 GATATC GAT^ATC
1198 GATATC
HapII 378 CCGG C^CGG
414 CCGG
HpaII 378 CCGG C^CGG
414 CCGG
MaeII 946 ACGT A^CGT
964 ACGT
MflI 735 GGATCC [GA]^GATC[CT]
1013 AGATCT
MseI 1181 TTAA T^TAA
1394 TTAA
MslI 639 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
798 CACGCCCATG
MspI 378 CCGG C^CGG
414 CCGG
SacI 925 GAGCTC GAGCT^C
957 GAGCTC
ScaI 338 AGTACT AGT^ACT
1046 AGTACT
Tru9I 1181 TTAA T^TAA
1394 TTAA
XhoII 735 GGATCC [GA]^GATC[CT]
1013 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I