Restriction map for
pFN21AE4212
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbrPI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SphI Sse8387I SspI
SwaI Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Alw44I 2000 GTGCAC G^TGCAC
ApaLI 2000 GTGCAC G^TGCAC
AspI 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1835 TGGCCA TGG^CCA
BamHI 1791 GGATCC G^GATCC
BbsI 112 GTCTTC GAAGAC(2/6)
BglII 854 AGATCT A^GATCT
BpuAI 112 GTCTTC GAAGAC(2/6)
BsaI 703 GGTCTC GGTCTC(1/5)
BsmAI 704 GTCTC GTCTC(1/5)
Bsp1407I 889 TGTACA T^GTACA
BsrGI 889 TGTACA T^GTACA
BstXI 1169 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 1786 GAAGAG CTCTTC(1/4)
EcoNI 2037 CCTAGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 829 GAATTC G^AATTC
HgaI 110 GCGTC GACGC(5/10)
HincII 656 GTCAAC GT[CT]^[GA]AC
HindII 656 GTCAAC GT[CT]^[GA]AC
Ksp632I 1786 GAAGAG CTCTTC(1/4)
KspI 129 CCGCGG CCGC^GG
MaeII 949 ACGT A^CGT
MluNI 1835 TGGCCA TGG^CCA
MscI 1835 TGGCCA TGG^CCA
MslI 1729 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2065 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1413 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 129 CCGCGG CCGC^GG
SgrAI 88 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1318 CTCAAG C^T[CT][GA]AG
SspBI 889 TGTACA T^GTACA
StuI 1353 AGGCCT AGG^CCT
Tth111I 1110 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2065 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 995 CCATTCCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 86 GGCGCC G[GA]^CG[CT]C
92 GGCGCC
AfaI 453 GTAC GT^AC
890 GTAC
AhdI 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2048 GACAAACTGTC
AlwI 1792 GATCC GGATC(4/5)
1791 GGATC
ApaI 281 GGGCCC GGGCC^C
665 GGGCCC
AspEI 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2048 GACAAACTGTC
BbeI 86 GGCGCC GGCGC^C
92 GGCGCC
BbvCI 294 CCTCAGC CCTCAGC(-5/-2)
873 CCTCAGC
BglI 71 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
878 GCCCCTCTGGC
BsaHI 86 GGCGCC G[GA]^CG[CT]C
92 GGCGCC
BsgI 387 GTGCAG GTGCAG(16/14)
812 GTGCAG
BssSI 312 CTCGTG CACGAG(-5/-1)
1589 CTCGTG
BstYI 854 AGATCT [GA]^GATC[CT]
1791 GGATCC
DpnII 855 GATC ^GATC
1792 GATC
Eam1105I 16 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2048 GACAAACTGTC
HaeII 86 GGCGCC [GA]GCGC^[CT]
92 GGCGCC
Hin1I 86 GGCGCC G[GA]^CG[CT]C
92 GGCGCC
KasI 86 GGCGCC G^GCGCC
92 GGCGCC
MboI 855 GATC ^GATC
1792 GATC
MflI 854 AGATCT [GA]^GATC[CT]
1791 GGATCC
NaeI 83 GCCGGC GCC^GGC
89 GCCGGC
NarI 86 GGCGCC GG^CGCC
92 GGCGCC
NcoI 910 CCATGG C^CATGG
1004 CCATGG
NdeI 1181 CATATG CA^TATG
2020 CATATG
NdeII 855 GATC ^GATC
1792 GATC
NgoMIV 83 GCCGGC G^CCGGC
89 GCCGGC
NotI 49 GCGGCCGC GC^GGCCGC
131 GCGGCCGC
NspI 45 ACATGC [GA]CATG^[CT]
640 ACATGC
RsaI 453 GTAC GT^AC
890 GTAC
Sau3AI 855 GATC ^GATC
1792 GATC
SfoI 86 GGCGCC GGC^GCC
92 GGCGCC
SmaI 143 CCCGGG CCC^GGG
225 CCCGGG
SpeI 1360 ACTAGT A^CTAGT
1375 ACTAGT
TaqI 194 TCGA T^CGA
1981 TCGA
TthHB8I 194 TCGA T^CGA
1981 TCGA
XhoII 854 AGATCT [GA]^GATC[CT]
1791 GGATCC
XmaCI 143 CCCGGG C^CCGGG
225 CCCGGG
XmaI 143 CCCGGG C^CCGGG
225 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PstI PvuII Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I Tsp509I TspRI