Restriction map for
pFN21AE4131
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FseI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 154 GTATAC GT^[AC][GT]AC
AccIII 433 TCCGGA T^CCGGA
AcsI 1119 AAATTT [GA]^AATT[CT]
AflIII 1092 ACATGT A^C[GA][CT]GT
AlwNI 85 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 336 CCTCAGG CC^T[ACGT]AGG
Aor51HI 138 AGCGCT AGC^GCT
ApoI 1119 AAATTT [GA]^AATT[CT]
AspHI 3 GAGCTC G[AT]GC[AT]^C
AviII 1341 TGCGCA TGC^GCA
BanII 3 GAGCTC G[GA]GC[CT]^C
BbsI 317 GAAGAC GAAGAC(2/6)
BclI 897 TGATCA T^GATCA
BfaI 284 CTAG C^TAG
BglII 1317 AGATCT A^GATCT
BpuAI 317 GAAGAC GAAGAC(2/6)
BsaBI 172 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 433 TCCGGA T^CCGGA
BseRI 429 CTCCTC GAGGAG(10/8)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsmAI 685 GTCTC GTCTC(1/5)
BspEI 433 TCCGGA T^CCGGA
BspHI 483 TCATGA T^CATGA
BspLU11I 1092 ACATGT A^CATGT
BsrI 551 ACTGG ACTGG(1/-1)
Bst1107I 154 GTATAC GTA^TAC
BstF5I 207 GGATG GGATG(2/0)
BstZ17I 154 GTATAC GTA^TAC
Bsu36I 336 CCTCAGG CC^T[ACGT]AGG
DraII 279 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 957 CGGCCA [CT]^GGCC[GA]
EarI 1060 GAAGAG CTCTTC(1/4)
Eco47III 138 AGCGCT AGC^GCT
Eco81I 336 CCTCAGG CC^T[ACGT]AGG
EcoO109I 279 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 870 ATGCAT ATGCA^T
FauI 413 CCCGC CCCGC(4/6)
FbaI 897 TGATCA T^GATCA
FokI 207 GGATG GGATG(9/13)
FspI 1341 TGCGCA TGC^GCA
HaeII 138 AGCGCT [GA]GCGC^[CT]
HincII 1153 GTCAAC GT[CT]^[GA]AC
HindII 1153 GTCAAC GT[CT]^[GA]AC
HphI 422 TCACC GGTGA(8/7)
Ksp632I 1060 GAAGAG CTCTTC(1/4)
MaeI 284 CTAG C^TAG
MamI 172 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 433 TCCGGA T^CCGGA
NdeI 1267 CATATG CA^TATG
NlaIV 15 GGAGCC GG[ACGT]^[ACGT]CC
NsiI 870 ATGCAT ATGCA^T
PacI 1390 TTAATTAA TTAAT^TAA
PflMI 232 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1014 GACTC GAGTC(4/5)
PvuII 1067 CAGCTG CAG^CTG
RcaI 483 TCATGA T^CATGA
SacI 3 GAGCTC GAGCT^C
SmlI 224 CTCAAG C^T[CT][GA]AG
SphI 868 GCATGC GCATG^C
SspI 818 AATATT AAT^ATT
StuI 1175 AGGCCT AGG^CCT
TfiI 779 GAATC G^A[AT]TC
Tsp45I 703 GTGAC ^GT[GC]AC
TspRI 1132 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 232 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BsaWI 433 TCCGGA [AT]^CCGG[AT]
917 TCCGGT
BsmFI 1089 GGGAC GGGAC(10/14)
99 GTCCC
DraI 622 TTTAAA TTT^AAA
711 TTTAAA
HapII 434 CCGG C^CGG
918 CCGG
HinfI 779 GAATC G^A[ACGT]TC
1014 GACTC
HpaII 434 CCGG C^CGG
918 CCGG
MspA1I 19 CCGCTG C[AC]G^C[GT]G
1067 CAGCTG
MspI 434 CCGG C^CGG
918 CCGG
NspI 868 GCATGC [GA]CATG^[CT]
1092 ACATGT
PstI 937 CTGCAG CTGCA^G
1321 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I XhoII