Restriction map for
pFN21AE3237
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AccI AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT14I FauI FbaI
FseI FspI HaeII HgaI HhaI
HinP1I HpaI KasI KpnI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 1553 GACGTC GACGT^C
AclI 824 AACGTT AA^CGTT
AcyI 1553 GACGTC G[GA]^CG[CT]C
AfaI 197 GTAC GT^AC
AvaII 958 GGTCC G^G[AT]CC
BalI 17 TGGCCA TGG^CCA
BcnI 100 CCCGG CC^[GC]GG
BsaBI 538 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1553 GACGTC G[GA]^CG[CT]C
BsaI 1112 GAGACC GGTCTC(1/5)
BsiYI 1640 CCAGCTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1640 CCAGCTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 463 CGTCTC CGTCTC(1/5)
BsmFI 1312 GTCCC GGGAC(10/14)
BsmI 1342 GAATGC GAATGC(1/-1)
BspMI 1215 GCAGGT ACCTGC(4/8)
BstBI 395 TTCGAA TT^CGAA
BstNI 210 CCAGG CC^[AT]GG
Cfr13I 958 GGTCC G^G[ACGT]CC
DraIII 436 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 46 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 62 CCGCGG C^C[GA][CT]GG
EaeI 17 TGGCCA [CT]^GGCC[GA]
EarI 1147 GAAGAG CTCTTC(1/4)
EcoRII 210 CCAGG ^CC[AT]GG
EcoRV 865 GATATC GAT^ATC
EcoT22I 801 ATGCAT ATGCA^T
HapII 101 CCGG C^CGG
Hin1I 1553 GACGTC G[GA]^CG[CT]C
HpaII 101 CCGG C^CGG
Ksp632I 1147 GAAGAG CTCTTC(1/4)
KspI 62 CCGCGG CCGC^GG
MamI 538 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 533 CAATTG C^AATTG
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
MslI 763 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 101 CCGG C^CGG
MunI 533 CAATTG C^AATTG
MvaI 210 CCAGG CC^[AT]GG
NciI 100 CCCGG CC^[GC]GG
NsiI 801 ATGCAT ATGCA^T
NspI 1607 ACATGC [GA]CATG^[CT]
NspV 395 TTCGAA TT^CGAA
PflMI 1640 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 824 AACGTT AA^CGTT
PvuII 1544 CAGCTG CAG^CTG
RsaI 197 GTAC GT^AC
SacII 62 CCGCGG CCGC^GG
Sau96I 958 GGTCC G^G[ACGT]CC
SfcI 119 CTATAG C^T[GA][CT]AG
SfuI 395 TTCGAA TT^CGAA
SspI 973 AATATT AAT^ATT
StuI 1387 AGGCCT AGG^CCT
Tsp45I 716 GTGAC ^GT[GC]AC
Van91I 1640 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1074 TCTAGA T^CTAGA
XcmI 297 CCAGATGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 63 CGCG CG^CG
415 CGCG
AciI 62 CCGC CCGC(-3/-1)
64 GCGG
AlwI 610 GATCC GGATC(4/5)
991 GGATC
BbsI 877 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BbvI 1636 GCAGC GCAGC(8/12)
1091 GCTGC
BpmI 1490 CTCCAG CTGGAG(16/14)
1109 CTGGAG
BpuAI 877 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BsaJI 62 CCGCGG C^C[ACGT][ACGT]GG
210 CCAGGG
BseRI 1252 GAGGAG GAGGAG(10/8)
1307 GAGGAG
BsmAI 1112 GAGAC GTCTC(1/5)
464 GTCTC
BssKI 100 CCCGG ^CC[ACGT]GG
210 CCAGG
BstUI 63 CGCG CG^CG
415 CGCG
BstYI 609 AGATCC [GA]^GATC[CT]
991 GGATCT
Fnu4HI 1091 GCTGC GC^[ACGT]GC
1636 GCAGC
HaeIII 18 GGCC GG^CC
1388 GGCC
HincII 222 GTTGAC GT[CT]^[GA]AC
1524 GTTGAC
HindII 222 GTTGAC GT[CT]^[GA]AC
1524 GTTGAC
HindIII 367 AAGCTT A^AGCTT
1193 AAGCTT
ItaI 1091 GCTGC GC^[ACGT]GC
1636 GCAGC
MflI 609 AGATCC [GA]^GATC[CT]
991 GGATCT
MspA1I 62 CCGCGG C[AC]G^C[GT]G
1544 CAGCTG
MvnI 63 CGCG CG^CG
415 CGCG
PleI 283 GAGTC GAGTC(4/5)
1310 GAGTC
ScrFI 100 CCCGG CC^[ACGT]GG
210 CCAGG
SfaNI 1159 GATGC GCATC(5/9)
616 GCATC
TaqI 396 TCGA T^CGA
1557 TCGA
TseI 1091 GCTGC G^C[AT]GC
1636 GCAGC
TthHB8I 396 TCGA T^CGA
1557 TCGA
XhoII 609 AGATCC [GA]^GATC[CT]
991 GGATCT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BfaI
BsrI BstF5I Cac8I DdeI DpnII
EcoRI FokI HinfI HphI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII Sau3AI
TfiI Tru9I Tsp509I TspRI XmnI