Restriction map for
pFN21AB9752
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbrPI
BbvCI BciVI BfrI BsaAI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FseI FspI HincII
HindII HpaI KpnI MfeI MluI
MluNI MroI MscI MunI NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 243 GACGTC GACGT^C
AcsI 1085 GAATTC [GA]^AATT[CT]
AflIII 1400 ACATGT A^C[GA][CT]GT
ApaI 1207 GGGCCC GGGCC^C
ApoI 1085 GAATTC [GA]^AATT[CT]
AvrII 1773 CCTAGG C^CTAGG
BbsI 904 GTCTTC GAAGAC(2/6)
BclI 476 TGATCA T^GATCA
BglII 524 AGATCT A^GATCT
BlnI 1773 CCTAGG C^CTAGG
BpuAI 904 GTCTTC GAAGAC(2/6)
Bsp1407I 665 TGTACA T^GTACA
BspHI 456 TCATGA T^CATGA
BspLU11I 1400 ACATGT A^CATGT
BsrBI 1263 CCGCTC CCGCTC(-3/-3)
BsrGI 665 TGTACA T^GTACA
BssHII 367 GCGCGC G^CGCGC
BstBI 615 TTCGAA TT^CGAA
BstXI 1125 CCAAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 130 GAAGAG CTCTTC(1/4)
EcoRI 1085 GAATTC G^AATTC
EcoRV 1075 GATATC GAT^ATC
EcoT22I 1034 ATGCAT ATGCA^T
FbaI 476 TGATCA T^GATCA
HgaI 334 GCGTC GACGC(5/10)
HindIII 1779 AAGCTT A^AGCTT
Ksp632I 130 GAAGAG CTCTTC(1/4)
MseI 1783 TTAA T^TAA
NsiI 1034 ATGCAT ATGCA^T
NspV 615 TTCGAA TT^CGAA
RcaI 456 TCATGA T^CATGA
SacI 439 GAGCTC GAGCT^C
SfuI 615 TTCGAA TT^CGAA
SmaI 385 CCCGGG CCC^GGG
SphI 32 GCATGC GCATG^C
SspBI 665 TGTACA T^GTACA
SspI 551 AATATT AAT^ATT
StuI 1269 AGGCCT AGG^CCT
Tru9I 1783 TTAA T^TAA
XcmI 743 CCAACAATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 385 CCCGGG C^CCGGG
XmaI 385 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AlwNI 896 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1572 CAGCCCCTG
AvaI 385 CCCGGG C^[CT]CG[GA]G
690 CTCGGG
BanI 79 GGCGCC G^G[CT][GA]CC
124 GGCGCC
BbeI 79 GGCGCC GGCGC^C
124 GGCGCC
BfaI 1766 CTAG C^TAG
1774 CTAG
BglI 127 GCCGAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
167 GCCGCGGCGGC
BlpI 36 GCTAAGC GC^T[ACGT]AGC
1731 GCTTAGC
Bpu1102I 36 GCTAAGC GC^T[ACGT]AGC
1731 GCTTAGC
BsaBI 474 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
958 GATAGGAATC
BseRI 1176 CTCCTC GAGGAG(10/8)
1185 CTCCTC
BsgI 1808 CTGCAC GTGCAG(16/14)
27 GTGCAG
BsmBI 206 GAGACG CGTCTC(1/5)
833 GAGACG
BsmI 883 GCATTC GAATGC(1/-1)
1036 GCATTC
BsoBI 385 CCCGGG C^[CT]CG[GA]G
690 CTCGGG
BspMI 1727 ACCTGC ACCTGC(4/8)
631 GCAGGT
BstAPI 631 GCAGGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1698 GCAGATGTTGC
CelII 36 GCTAAGC GC^T[ACGT]AGC
1731 GCTTAGC
DraIII 656 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1673 CACCGAGTG
EcoNI 1310 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1711 CCTGTGGAAGG
KasI 79 GGCGCC G^GCGCC
124 GGCGCC
MaeI 1766 CTAG C^TAG
1774 CTAG
MaeII 244 ACGT A^CGT
812 ACGT
MaeIII 336 GTCAC ^GT[ACGT]AC
916 GTCAC
MamI 474 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
958 GATAGGAATC
NarI 79 GGCGCC GG^CGCC
124 GGCGCC
NcoI 563 CCATGG C^CATGG
1406 CCATGG
NdeI 1031 CATATG CA^TATG
1813 CATATG
PstI 162 CTGCAG CTGCA^G
427 CTGCAG
PvuII 1222 CAGCTG CAG^CTG
1434 CAGCTG
SfcI 162 CTGCAG C^T[GA][CT]AG
427 CTGCAG
SfoI 79 GGCGCC GGC^GCC
124 GGCGCC
SmlI 43 CTCAAG C^T[CT][GA]AG
1799 CTCAAG
TaqI 616 TCGA T^CGA
725 TCGA
Tsp45I 336 GTCAC ^GT[GC]AC
916 GTCAC
Tsp509I 1086 AATT ^AATT
1638 AATT
TthHB8I 616 TCGA T^CGA
725 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaLI AspHI AvaII
BanII BbvI BcgI BcnI BmyI
BpmI BsaHI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI RsaI SacII Sau3AI
Sau96I ScrFI StyI TfiI TseI
TspRI XhoII