Restriction map for
pFN21AB6247
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccII AclI AflII
AflIII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BclI BcnI BfrI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
BsmBI BsoBI BspHI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CpoI DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI HgaI HpaI
KpnI Ksp632I KspI MaeII MamI
MluI MvnI NciI NcoI NheI
NotI NruI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 712 GTAGAC GT^[AC][GT]AC
AccIII 329 TCCGGA T^CCGGA
AcyI 68 GGCGCC G[GA]^CG[CT]C
AhdI 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 259 GTGCAC G^TGCAC
AocI 2237 CCTCAGG CC^T[ACGT]AGG
ApaLI 259 GTGCAC G^TGCAC
AspEI 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1197 TGGCCA TGG^CCA
BbeI 68 GGCGCC GGCGC^C
BciVI 684 GGATAC GTATCC(6/5)
BfaI 1000 CTAG C^TAG
BglI 802 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 521 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 521 GCTCAGC GC^T[ACGT]AGC
BsaHI 68 GGCGCC G[GA]^CG[CT]C
BsaI 641 GAGACC GGTCTC(1/5)
BseAI 329 TCCGGA T^CCGGA
Bsp1407I 2121 TGTACA T^GTACA
BspDI 1148 ATCGAT AT^CGAT
BspEI 329 TCCGGA T^CCGGA
BspMI 811 GCAGGT ACCTGC(4/8)
BsrFI 218 GCCGGC [GA]^CCGG[CT]
BsrGI 2121 TGTACA T^GTACA
BssSI 187 CACGAG CACGAG(-5/-1)
BstBI 1514 TTCGAA TT^CGAA
BstXI 2027 CCAGCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 275 AGATCC [GA]^GATC[CT]
Bsu36I 2237 CCTCAGG CC^T[ACGT]AGG
CelII 521 GCTCAGC GC^T[ACGT]AGC
CfoI 69 GCGC GCG^C
Cfr10I 218 GCCGGC [GA]^CCGG[CT]
ClaI 1148 ATCGAT AT^CGAT
EaeI 1197 TGGCCA [CT]^GGCC[GA]
Eam1105I 1535 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2237 CCTCAGG CC^T[ACGT]AGG
EcoT22I 451 ATGCAT ATGCA^T
HaeII 68 GGCGCC [GA]GCGC^[CT]
HhaI 69 GCGC GCG^C
Hin1I 68 GGCGCC G[GA]^CG[CT]C
HinP1I 69 GCGC G^CGC
HincII 840 GTTGAC GT[CT]^[GA]AC
HindII 840 GTTGAC GT[CT]^[GA]AC
KasI 68 GGCGCC G^GCGCC
MaeI 1000 CTAG C^TAG
MfeI 175 CAATTG C^AATTG
MflI 275 AGATCC [GA]^GATC[CT]
MluNI 1197 TGGCCA TGG^CCA
MroI 329 TCCGGA T^CCGGA
MscI 1197 TGGCCA TGG^CCA
MunI 175 CAATTG C^AATTG
NaeI 218 GCCGGC GCC^GGC
NarI 68 GGCGCC GG^CGCC
NdeI 695 CATATG CA^TATG
NgoMIV 218 GCCGGC G^CCGGC
NsiI 451 ATGCAT ATGCA^T
NspV 1514 TTCGAA TT^CGAA
PflMI 792 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 343 CAGCTG CAG^CTG
SfoI 68 GGCGCC GGC^GCC
SfuI 1514 TTCGAA TT^CGAA
SspBI 2121 TGTACA T^GTACA
Tsp45I 2102 GTGAC ^GT[GC]AC
Van91I 792 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 275 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 202 CCGC CCGC(-3/-1)
237 CCGC
BanI 68 GGCGCC G^G[CT][GA]CC
1871 GGCACC
BbsI 1251 GAAGAC GAAGAC(2/6)
1098 GTCTTC
BpuAI 1251 GAAGAC GAAGAC(2/6)
1098 GTCTTC
BsaWI 329 TCCGGA [AT]^CCGG[AT]
2140 ACCGGA
BsgI 1546 CTGCAC GTGCAG(16/14)
340 GTGCAG
DraI 862 TTTAAA TTT^AAA
1128 TTTAAA
EcoRI 1796 GAATTC G^AATTC
1964 GAATTC
EcoT14I 266 CCAAGG C^C[AT][AT]GG
806 CCTTGG
HindIII 1169 AAGCTT A^AGCTT
2093 AAGCTT
MaeIII 182 GTTAC ^GT[ACGT]AC
2102 GTGAC
MspA1I 237 CCGCTG C[AC]G^C[GT]G
343 CAGCTG
PpuMI 701 AGGACCT [GA]G^G[AT]CC[CT]
1541 AGGTCCT
PstI 8 CTGCAG CTGCA^G
763 CTGCAG
SacI 229 GAGCTC GAGCT^C
325 GAGCTC
SmlI 295 CTTGAG C^T[CT][GA]AG
623 CTCAAG
StuI 27 AGGCCT AGG^CCT
1337 AGGCCT
StyI 266 CCAAGG C^C[AT][AT]GG
806 CCTTGG
XcmI 186 CCACGAGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
570 CCACCTCCCCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI