Restriction map for
pFN21AE3721
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI ApoI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV FauI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MunI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 265 GTAGAC GT^[AC][GT]AC
AflIII 824 ACATGT A^C[GA][CT]GT
AocI 484 CCTAAGG CC^T[ACGT]AGG
Aor51HI 371 AGCGCT AGC^GCT
AseI 565 ATTAAT AT^TAAT
AsnI 565 ATTAAT AT^TAAT
Asp700I 738 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 208 GAGCAC G[AT]GC[AT]^C
BanI 248 GGCACC G^G[CT][GA]CC
BclI 107 TGATCA T^GATCA
BcnI 534 CCGGG CC^[GC]GG
BsaBI 345 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 507 ACCGGA [AT]^CCGG[AT]
BseRI 156 CTCCTC GAGGAG(10/8)
BsiEI 461 CGGTCG CG[GA][CT]^CG
BsiHKAI 208 GAGCAC G[AT]GC[AT]^C
BsmFI 833 GGGAC GGGAC(10/14)
BsmI 54 GAATGC GAATGC(1/-1)
Bsp1407I 842 TGTACA T^GTACA
BspLU11I 824 ACATGT A^CATGT
BsrDI 786 CATTGC GCAATG(2/0)
BsrFI 1220 GCCGGC [GA]^CCGG[CT]
BsrGI 842 TGTACA T^GTACA
BssSI 1020 CACGAG CACGAG(-5/-1)
BstEII 20 GGTCACC G^GT[ACGT]ACC
BstPI 20 GGTCACC G^GT[ACGT]ACC
BstYI 350 AGATCC [GA]^GATC[CT]
Bsu36I 484 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1220 GCCGGC [GA]^CCGG[CT]
Cfr13I 545 GGGCC G^G[ACGT]CC
DsaI 386 CCATGG C^C[GA][CT]GG
EarI 1027 CTCTTC CTCTTC(1/4)
Eco47III 371 AGCGCT AGC^GCT
Eco81I 484 CCTAAGG CC^T[ACGT]AGG
EcoO65I 20 GGTCACC G^GT[ACGT]ACC
EcoT22I 718 ATGCAT ATGCA^T
FbaI 107 TGATCA T^GATCA
HaeII 371 AGCGCT [GA]GCGC^[CT]
Ksp632I 1027 CTCTTC CTCTTC(1/4)
MaeII 995 ACGT A^CGT
MamI 345 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 350 AGATCC [GA]^GATC[CT]
NaeI 1220 GCCGGC GCC^GGC
NciI 534 CCGGG CC^[GC]GG
NcoI 386 CCATGG C^CATGG
NgoMIV 1220 GCCGGC G^CCGGC
NsiI 718 ATGCAT ATGCA^T
NspI 824 ACATGT [GA]CATG^[CT]
PshBI 565 ATTAAT AT^TAAT
Sau96I 545 GGGCC G^G[ACGT]CC
ScaI 1124 AGTACT AGT^ACT
SexAI 711 ACCTGGT A^CC[AT]GGT
SspBI 842 TGTACA T^GTACA
TaqI 1158 TCGA T^CGA
TfiI 953 GAATC G^A[AT]TC
Tsp45I 21 GTCAC ^GT[GC]AC
TthHB8I 1158 TCGA T^CGA
XcmI 356 CCATGCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 350 AGATCC [GA]^GATC[CT]
XmnI 738 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 174 GCGG CCGC(-3/-1)
848 GCGG
AlwI 351 GATCC GGATC(4/5)
576 GATCC
AlwNI 601 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
707 CAGTACCTG
BmyI 208 GAGCAC G[AGT]GC[ACT]^C
247 GGGCAC
BsgI 493 GTGCAG GTGCAG(16/14)
538 GTGCAG
Bsp1286I 208 GAGCAC G[AGT]GC[ACT]^C
247 GGGCAC
BstF5I 270 CATCC GGATG(2/0)
317 GGATG
BstXI 26 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
237 CCATGCCAATGG
CfoI 372 GCGC GCG^C
973 GCGC
EcoT14I 386 CCATGG C^C[AT][AT]GG
1143 CCAAGG
FokI 270 CATCC GGATG(9/13)
317 GGATG
HhaI 372 GCGC GCG^C
973 GCGC
HinP1I 372 GCGC G^CGC
973 GCGC
HindIII 739 AAGCTT A^AGCTT
1073 AAGCTT
MaeIII 21 GTCAC ^GT[ACGT]AC
694 GTAAC
MspA1I 172 CAGCGG C[AC]G^C[GT]G
846 CAGCGG
NlaIV 176 GGCTCC GG[ACGT]^[ACGT]CC
248 GGCACC
SfaNI 318 GATGC GCATC(5/9)
296 GCATC
StyI 386 CCATGG C^C[AT][AT]GG
1143 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI BalI BbvI BpmI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI Cac8I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII PflMI PleI RsaI Sau3AI
ScrFI Tru9I TseI Tsp509I TspRI
Van91I