Restriction map for
pFN21AE3042
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MaeI MamI
MfeI MflI MluI MroI MslI
MunI MvaI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 180 GGCGTC G[GA]^CG[CT]C
AluI 263 AGCT AG^CT
AlwNI 188 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 461 TGGCCA TGG^CCA
BanII 76 GGGCTC G[GA]GC[CT]^C
BbvCI 185 CCTCAGC CCTCAGC(-5/-2)
BmyI 76 GGGCTC G[AGT]GC[ACT]^C
BpmI 217 CTGGAG CTGGAG(16/14)
BsaHI 180 GGCGTC G[GA]^CG[CT]C
Bsp1286I 76 GGGCTC G[AGT]GC[ACT]^C
BstF5I 375 GGATG GGATG(2/0)
DdeI 186 CTCAG C^T[ACGT]AG
DpnII 447 GATC ^GATC
EarI 520 GAAGAG CTCTTC(1/4)
EcoRI 525 GAATTC G^AATTC
FokI 375 GGATG GGATG(9/13)
HaeII 207 AGCGCC [GA]GCGC^[CT]
HgaI 181 GCGTC GACGC(5/10)
Hin1I 180 GGCGTC G[GA]^CG[CT]C
HphI 320 TCACC GGTGA(8/7)
Ksp632I 520 GAAGAG CTCTTC(1/4)
MaeII 458 ACGT A^CGT
MaeIII 319 GTCAC ^GT[ACGT]AC
MboI 447 GATC ^GATC
MluNI 461 TGGCCA TGG^CCA
MscI 461 TGGCCA TGG^CCA
MseI 452 TTAA T^TAA
NdeII 447 GATC ^GATC
PstI 488 CTGCAG CTGCA^G
PvuII 262 CAGCTG CAG^CTG
Sau3AI 447 GATC ^GATC
ScaI 368 AGTACT AGT^ACT
SfcI 488 CTGCAG C^T[GA][CT]AG
SmaI 73 CCCGGG CCC^GGG
Tru9I 452 TTAA T^TAA
Tsp45I 319 GTCAC ^GT[GC]AC
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 466 AAATTC [GA]^AATT[CT]
525 GAATTC
AfaI 237 GTAC GT^AC
369 GTAC
ApoI 466 AAATTC [GA]^AATT[CT]
525 GAATTC
AvaI 34 CCCGAG C^[CT]CG[GA]G
73 CCCGGG
AvaII 30 GGACC G^G[AT]CC
69 GGTCC
BbvI 261 GCAGC GCAGC(8/12)
90 GCTGC
BsaI 496 GAGACC GGTCTC(1/5)
148 GGTCTC
BsmAI 496 GAGAC GTCTC(1/5)
149 GTCTC
BsoBI 34 CCCGAG C^[CT]CG[GA]G
73 CCCGGG
BsrFI 43 GCCGGC [GA]^CCGG[CT]
93 GCCGGC
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
93 GCCGGC
DsaI 65 CCGCGG C^C[GA][CT]GG
323 CCGCGG
FauI 64 CCCGC CCCGC(4/6)
99 GCGGG
KspI 65 CCGCGG CCGC^GG
323 CCGCGG
MboII 517 GAAGA GAAGA(8/7)
520 GAAGA
NaeI 43 GCCGGC GCC^GGC
93 GCCGGC
NgoMIV 43 GCCGGC G^CCGGC
93 GCCGGC
NlaIII 258 CATG CATG^
297 CATG
RsaI 237 GTAC GT^AC
369 GTAC
SacII 65 CCGCGG CCGC^GG
323 CCGCGG
SfaNI 253 GATGC GCATC(5/9)
190 GCATC
TseI 90 GCTGC G^C[AT]GC
261 GCAGC
Tsp509I 467 AATT ^AATT
526 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI BcnI BsaJI BsiYI
BslI BsmFI BssKI BstUI Cac8I
CfoI Cfr13I EaeI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV PleI Sau96I
ScrFI