Restriction map for
pFN21AE4123
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbrPI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRI
EcoT22I FspI HincII HindII HpaI
KpnI KspI MaeII MfeI MluI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 1064 CGCG CG^CG
AcsI 449 AAATTC [GA]^AATT[CT]
AfaI 621 GTAC GT^AC
AlwNI 221 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1017 CCTGAGG CC^T[ACGT]AGG
Aor51HI 637 AGCGCT AGC^GCT
ApoI 449 AAATTC [GA]^AATT[CT]
AspHI 859 GTGCTC G[AT]GC[AT]^C
AvaI 1090 CTCGGG C^[CT]CG[GA]G
BalI 1010 TGGCCA TGG^CCA
BanI 455 GGCGCC G^G[CT][GA]CC
BbeI 455 GGCGCC GGCGC^C
BbsI 1042 GTCTTC GAAGAC(2/6)
BbvCI 1186 GCTGAGG CCTCAGC(-5/-2)
BclI 263 TGATCA T^GATCA
BglI 107 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1042 GTCTTC GAAGAC(2/6)
BsaBI 150 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 863 TCCGGT [AT]^CCGG[AT]
BseRI 610 CTCCTC GAGGAG(10/8)
BsgI 1172 GTGCAG GTGCAG(16/14)
BsiHKAI 859 GTGCTC G[AT]GC[AT]^C
BsmBI 178 GAGACG CGTCTC(1/5)
BsmI 1220 GCATTC GAATGC(1/-1)
BsoBI 1090 CTCGGG C^[CT]CG[GA]G
BspHI 908 TCATGA T^CATGA
BspMI 829 ACCTGC ACCTGC(4/8)
BstEII 1248 GGTCACC G^GT[ACGT]ACC
BstPI 1248 GGTCACC G^GT[ACGT]ACC
BstUI 1064 CGCG CG^CG
BstXI 435 CCAATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1017 CCTGAGG CC^T[ACGT]AGG
Cac8I 116 GCCGGC GC[ACGT]^[ACGT]GC
DraIII 1314 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 637 AGCGCT AGC^GCT
Eco81I 1017 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1248 GGTCACC G^GT[ACGT]ACC
EcoRV 478 GATATC GAT^ATC
FbaI 263 TGATCA T^GATCA
FseI 115 GGCCGGCC GGCCGG^CC
HgaI 1108 GCGTC GACGC(5/10)
HindIII 193 AAGCTT A^AGCTT
KasI 455 GGCGCC G^GCGCC
MamI 150 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1010 TGGCCA TGG^CCA
MscI 1010 TGGCCA TGG^CCA
MseI 1490 TTAA T^TAA
MspA1I 256 CAGCGG C[AC]G^C[GT]G
MvnI 1064 CGCG CG^CG
NaeI 116 GCCGGC GCC^GGC
NarI 455 GGCGCC GG^CGCC
NcoI 524 CCATGG C^CATGG
NgoMIV 116 GCCGGC G^CCGGC
PpuMI 1334 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1081 CTGCAG CTGCA^G
RcaI 908 TCATGA T^CATGA
RsaI 621 GTAC GT^AC
SapI 70 GAAGAGC GCTCTTC(1/4)
SfoI 455 GGCGCC GGC^GCC
SmlI 1464 CTCAAG C^T[CT][GA]AG
Tru9I 1490 TTAA T^TAA
Tsp45I 1249 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 825 GTCTAC GT^[AC][GT]AC
1177 GTCTAC
AcyI 455 GGCGCC G[GA]^CG[CT]C
1107 GGCGTC
AflIII 1001 ACATGT A^C[GA][CT]GT
1277 ACATGT
BpmI 1167 CTCCAG CTGGAG(16/14)
703 CTGGAG
BsaHI 455 GGCGCC G[GA]^CG[CT]C
1107 GGCGTC
BspLU11I 1001 ACATGT A^CATGT
1277 ACATGT
BsrFI 116 GCCGGC [GA]^CCGG[CT]
401 ACCGGC
BstYI 149 AGATCC [GA]^GATC[CT]
1085 AGATCC
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
401 ACCGGC
DraII 222 AGGGCCT [GA]G^G[ACGT]CC[CT]
1334 GGGTCCT
DsaI 524 CCATGG C^C[GA][CT]GG
656 CCGTGG
EaeI 653 TGGCCG [CT]^GGCC[GA]
1010 TGGCCA
EcoO109I 222 AGGGCCT [GA]G^G[ACGT]CC[CT]
1334 GGGTCCT
EcoT14I 524 CCATGG C^C[AT][AT]GG
1073 CCAAGG
FauI 258 GCGGG CCCGC(4/6)
1116 GCGGG
HaeII 455 GGCGCC [GA]GCGC^[CT]
637 AGCGCT
Hin1I 455 GGCGCC G[GA]^CG[CT]C
1107 GGCGTC
MaeIII 398 GTAAC ^GT[ACGT]AC
1249 GTCAC
MflI 149 AGATCC [GA]^GATC[CT]
1085 AGATCC
MwoI 107 GCCCAAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
640 GCTGCCCGTGC
NspI 1001 ACATGT [GA]CATG^[CT]
1277 ACATGT
SfcI 1081 CTGCAG C^T[GA][CT]AG
1262 CTGTAG
StyI 524 CCATGG C^C[AT][AT]GG
1073 CCAAGG
TaqI 95 TCGA T^CGA
851 TCGA
TfiI 419 GAATC G^A[AT]TC
1445 GAATC
TthHB8I 95 TCGA T^CGA
851 TCGA
XcmI 692 CCATCTTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1112 CCATGCGGGAGTTGG
XhoII 149 AGATCC [GA]^GATC[CT]
1085 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MspI MvaI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp509I TspRI