Restriction map for
pFN21AE9508
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvrII BalI
BbeI BbrPI BciVI BclI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 386 GGTACC G^GTACC
AcsI 732 GAATTT [GA]^AATT[CT]
Aor51HI 356 AGCGCT AGC^GCT
ApaI 707 GGGCCC GGGCC^C
ApoI 732 GAATTT [GA]^AATT[CT]
Asp718I 386 GGTACC G^GTACC
AspHI 142 GTGCTC G[AT]GC[AT]^C
AvaII 588 GGTCC G^G[AT]CC
AviII 167 TGCGCA TGC^GCA
BanI 386 GGTACC G^G[CT][GA]CC
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BcgI 284 GCAAAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 617 CCCGG CC^[GC]GG
BfaI 509 CTAG C^TAG
BlpI 336 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC
BsaAI 643 TACGTG [CT]AC^GT[GA]
BsaJI 153 CCTGGG C^C[ACGT][ACGT]GG
BsiEI 419 CGGTCG CG[GA][CT]^CG
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
BsmI 698 GCATTC GAATGC(1/-1)
BstXI 538 CCAGTCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 336 GCTGAGC GC^T[ACGT]AGC
EaeI 203 TGGCCG [CT]^GGCC[GA]
EarI 220 CTCTTC CTCTTC(1/4)
Eco47III 356 AGCGCT AGC^GCT
FspI 167 TGCGCA TGC^GCA
HapII 618 CCGG C^CGG
HgaI 840 GCGTC GACGC(5/10)
HpaII 618 CCGG C^CGG
HphI 910 GGTGA GGTGA(8/7)
KpnI 386 GGTACC GGTAC^C
Ksp632I 220 CTCTTC CTCTTC(1/4)
MaeI 509 CTAG C^TAG
MspI 618 CCGG C^CGG
NciI 617 CCCGG CC^[GC]GG
PleI 60 GACTC GAGTC(4/5)
PstI 883 CTGCAG CTGCA^G
SfcI 883 CTGCAG C^T[GA][CT]AG
SphI 164 GCATGC GCATG^C
StuI 324 AGGCCT AGG^CCT
TfiI 505 GATTC G^A[AT]TC
TspRI 662 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 135 CGCG CG^CG
543 CGCG
AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
853 CAGAACCTG
AocI 895 CCTGAGG CC^T[ACGT]AGG
925 CCTGAGG
BbsI 19 GTCTTC GAAGAC(2/6)
670 GTCTTC
BpuAI 19 GTCTTC GAAGAC(2/6)
670 GTCTTC
BseRI 676 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsmAI 58 GAGAC GTCTC(1/5)
181 GTCTC
BsrI 16 CCAGT ACTGG(1/-1)
538 CCAGT
BstUI 135 CGCG CG^CG
543 CGCG
Bsu36I 895 CCTGAGG CC^T[ACGT]AGG
925 CCTGAGG
Cac8I 164 GCATGC GC[ACGT]^[ACGT]GC
686 GCCTGC
Eco81I 895 CCTGAGG CC^T[ACGT]AGG
925 CCTGAGG
HaeII 340 AGCGCC [GA]GCGC^[CT]
356 AGCGCT
HinfI 60 GACTC G^A[ACGT]TC
505 GATTC
MaeIII 482 GTGAC ^GT[ACGT]AC
646 GTGAC
MvnI 135 CGCG CG^CG
543 CGCG
NspI 164 GCATGC [GA]CATG^[CT]
770 GCATGT
Tsp45I 482 GTGAC ^GT[GC]AC
646 GTGAC
Tsp509I 714 AATT ^AATT
733 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BamHI
BanII BbvI BmyI BpmI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstYI CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I ItaI
MaeII MboI MboII MflI MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI XhoII