Restriction map for
pFN21AE9622
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI KspI MamI MfeI MflI
MluI MroI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 537 GTCTAC GT^[AC][GT]AC
AciI 415 CCGC CCGC(-3/-1)
AclI 789 AACGTT AA^CGTT
AflIII 277 ACATGT A^C[GA][CT]GT
Alw44I 1431 GTGCAC G^TGCAC
ApaLI 1431 GTGCAC G^TGCAC
BalI 2 TGGCCA TGG^CCA
BbvI 121 GCTGC GCAGC(8/12)
BciVI 1347 GTATCC GTATCC(6/5)
BsgI 122 CTGCAC GTGCAG(16/14)
BsmFI 1487 GGGAC GGGAC(10/14)
Bsp1407I 720 TGTACA T^GTACA
BspLU11I 277 ACATGT A^CATGT
BsrDI 1058 GCAATG GCAATG(2/0)
BsrGI 720 TGTACA T^GTACA
BssSI 1176 CACGAG CACGAG(-5/-1)
BstXI 638 CCAGTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 475 GATC ^GATC
DraI 582 TTTAAA TTT^AAA
DraII 1487 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO109I 1487 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 5 CCAAGG C^C[AT][AT]GG
EcoT22I 290 ATGCAT ATGCA^T
Fnu4HI 121 GCTGC GC^[ACGT]GC
HincII 757 GTTAAC GT[CT]^[GA]AC
HindII 757 GTTAAC GT[CT]^[GA]AC
HpaI 757 GTTAAC GTT^AAC
ItaI 121 GCTGC GC^[ACGT]GC
MboI 475 GATC ^GATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 250 CAGCTG C[AC]G^C[GT]G
NdeII 475 GATC ^GATC
NsiI 290 ATGCAT ATGCA^T
PpuMI 1487 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 789 AACGTT AA^CGTT
PvuII 250 CAGCTG CAG^CTG
Sau3AI 475 GATC ^GATC
SpeI 693 ACTAGT A^CTAGT
SspBI 720 TGTACA T^GTACA
StuI 1204 AGGCCT AGG^CCT
StyI 5 CCAAGG C^C[AT][AT]GG
TseI 121 GCTGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 721 GTAC GT^AC
1161 GTAC
Asp700I 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1367 GAAGACTTTC
AspHI 1220 GAGCAC G[AT]GC[AT]^C
1431 GTGCAC
BanII 1456 GAGCCC G[GA]GC[CT]^C
1565 GAGCCC
BpmI 387 CTCCAG CTGGAG(16/14)
1570 CTGGAG
BsaJI 5 CCAAGG C^C[ACGT][ACGT]GG
1568 CCCTGG
BsiHKAI 1220 GAGCAC G[AT]GC[AT]^C
1431 GTGCAC
BsiYI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1404 CCCAGCAGTGG
BslI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1404 CCCAGCAGTGG
BsmBI 1246 GAGACG CGTCTC(1/5)
1548 GAGACG
BstBI 667 TTCGAA TT^CGAA
1470 TTCGAA
BstF5I 243 CATCC GGATG(2/0)
624 GGATG
Cac8I 1399 GCAAGC GC[ACGT]^[ACGT]GC
1438 GCCAGC
EcoRI 664 GAATTC G^AATTC
1515 GAATTC
EcoRV 238 GATATC GAT^ATC
1327 GATATC
FokI 243 CATCC GGATG(9/13)
624 GGATG
HaeIII 3 GGCC GG^CC
1205 GGCC
NspI 277 ACATGT [GA]CATG^[CT]
701 ACATGC
NspV 667 TTCGAA TT^CGAA
1470 TTCGAA
PleI 1063 GACTC GAGTC(4/5)
1087 GAGTC
RsaI 721 GTAC GT^AC
1161 GTAC
SfaNI 14 GATGC GCATC(5/9)
997 GATGC
SfcI 247 CTACAG C^T[GA][CT]AG
1644 CTGTAG
SfuI 667 TTCGAA TT^CGAA
1470 TTCGAA
SmlI 494 CTCAAG C^T[CT][GA]AG
1677 CTTGAG
Tsp45I 1198 GTCAC ^GT[GC]AC
1305 GTCAC
XmnI 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1367 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbsI
BfaI BmyI BpuAI BsmAI Bsp1286I
BspMI BsrI BssKI BstNI Cfr13I
DdeI EarI EcoRII HinfI HphI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I