Restriction map for
pFN21AB7681
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MaeII MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 749 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 10 GAGCAC G[AT]GC[AT]^C
AviII 563 TGCGCA TGC^GCA
BanI 858 GGTGCC G^G[CT][GA]CC
BsiHKAI 10 GAGCAC G[AT]GC[AT]^C
BsrFI 189 GCCGGC [GA]^CCGG[CT]
BssHII 17 GCGCGC G^CGCGC
Cfr10I 189 GCCGGC [GA]^CCGG[CT]
Eam1105I 955 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 573 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 807 ATGCAT ATGCA^T
FspI 563 TGCGCA TGC^GCA
HgaI 459 GCGTC GACGC(5/10)
NaeI 189 GCCGGC GCC^GGC
NgoMIV 189 GCCGGC G^CCGGC
NsiI 807 ATGCAT ATGCA^T
NspI 809 GCATGT [GA]CATG^[CT]
PflMI 748 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT]
SbfI 213 CCTGCAGG CCTGCA^GG
Sse8387I 213 CCTGCAGG CCTGCA^GG
Tsp45I 953 GTGAC ^GT[GC]AC
TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 748 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 648 GATCC GGATC(4/5)
751 GGATC
Asp700I 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
663 GAACCCCTTC
BalI 386 TGGCCA TGG^CCA
437 TGGCCA
BbsI 285 GAAGAC GAAGAC(2/6)
511 GTCTTC
BbvCI 718 CCTCAGC CCTCAGC(-5/-2)
277 GCTGAGG
BglI 329 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
761 GCCCTGGAGGC
BpuAI 285 GAAGAC GAAGAC(2/6)
511 GTCTTC
BseRI 280 GAGGAG GAGGAG(10/8)
496 GAGGAG
BsgI 38 CTGCAC GTGCAG(16/14)
112 CTGCAC
BsmBI 975 CGTCTC CGTCTC(1/5)
344 GAGACG
BsmFI 65 GGGAC GGGAC(10/14)
414 GGGAC
BspMI 110 ACCTGC ACCTGC(4/8)
212 ACCTGC
BsrI 1019 ACTGG ACTGG(1/-1)
743 CCAGT
BstF5I 795 CATCC GGATG(2/0)
897 CATCC
BstXI 428 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCACCCCCATGG
BstYI 647 AGATCC [GA]^GATC[CT]
751 GGATCT
DpnII 648 GATC ^GATC
752 GATC
FokI 795 CATCC GGATG(9/13)
897 CATCC
HinfI 416 GACTC G^A[ACGT]TC
424 GACTC
MaeIII 464 GTAAC ^GT[ACGT]AC
953 GTGAC
MboI 648 GATC ^GATC
752 GATC
MflI 647 AGATCC [GA]^GATC[CT]
751 GGATCT
MluNI 386 TGGCCA TGG^CCA
437 TGGCCA
MscI 386 TGGCCA TGG^CCA
437 TGGCCA
NdeII 648 GATC ^GATC
752 GATC
PleI 416 GACTC GAGTC(4/5)
424 GACTC
PstI 214 CTGCAG CTGCA^G
724 CTGCAG
Sau3AI 648 GATC ^GATC
752 GATC
SfcI 214 CTGCAG C^T[GA][CT]AG
724 CTGCAG
XhoII 647 AGATCC [GA]^GATC[CT]
751 GGATCT
XmnI 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
663 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
KspI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NlaIII NlaIV PvuII RsaI
SacII Sau96I ScrFI SfaNI SmaI
StyI TseI XmaCI XmaI