Restriction map for
pFN21AE4095
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 3 GTCTAC GT^[AC][GT]AC
AcsI 847 AAATTC [GA]^AATT[CT]
AlwI 909 GGATC GGATC(4/5)
ApaI 183 GGGCCC GGGCC^C
ApoI 847 AAATTC [GA]^AATT[CT]
BalI 221 TGGCCA TGG^CCA
BbsI 1225 GTCTTC GAAGAC(2/6)
BcgI 677 GCAAAATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 960 CCCGG CC^[GC]GG
BfaI 598 CTAG C^TAG
BpuAI 1225 GTCTTC GAAGAC(2/6)
BsaI 1170 GGTCTC GGTCTC(1/5)
BsaWI 242 TCCGGT [AT]^CCGG[AT]
BsmI 1255 GCATTC GAATGC(1/-1)
BssSI 895 CTCGTG CACGAG(-5/-1)
BstBI 721 TTCGAA TT^CGAA
BstXI 351 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1140 TTTAAA TTT^AAA
DraIII 84 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 357 CCATGG C^C[GA][CT]GG
EaeI 221 TGGCCA [CT]^GGCC[GA]
HgaI 207 GCGTC GACGC(5/10)
MaeI 598 CTAG C^TAG
MluNI 221 TGGCCA TGG^CCA
MscI 221 TGGCCA TGG^CCA
MseI 1141 TTAA T^TAA
NciI 960 CCCGG CC^[GC]GG
NcoI 357 CCATGG C^CATGG
NspV 721 TTCGAA TT^CGAA
PstI 256 CTGCAG CTGCA^G
SfuI 721 TTCGAA TT^CGAA
StuI 1324 AGGCCT AGG^CCT
TaqI 722 TCGA T^CGA
Tru9I 1141 TTAA T^TAA
Tsp509I 848 AATT ^AATT
TthHB8I 722 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 466 GTAC GT^AC
995 GTAC
BanII 183 GGGCCC G[GA]GC[CT]^C
746 GAGCCC
BbvCI 391 GCTGAGG CCTCAGC(-5/-2)
630 GCTGAGG
BglII 12 AGATCT A^GATCT
1001 AGATCT
CfoI 310 GCGC GCG^C
338 GCGC
EcoNI 27 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1180 CCTTTGAAAGG
FauI 186 CCCGC CCCGC(4/6)
1296 CCCGC
HaeII 309 GGCGCT [GA]GCGC^[CT]
337 AGCGCC
HapII 243 CCGG C^CGG
961 CCGG
HhaI 310 GCGC GCG^C
338 GCGC
HinP1I 310 GCGC G^CGC
338 GCGC
HpaII 243 CCGG C^CGG
961 CCGG
MaeII 1196 ACGT A^CGT
1400 ACGT
MaeIII 79 GTGAC ^GT[ACGT]AC
1176 GTCAC
MslI 546 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
822 CACGGGAATG
MspI 243 CCGG C^CGG
961 CCGG
PpuMI 172 AGGACCT [GA]G^G[AT]CC[CT]
1118 GGGACCT
RsaI 466 GTAC GT^AC
995 GTAC
SmlI 702 CTCAAG C^T[CT][GA]AG
730 CTTGAG
Tsp45I 79 GTGAC ^GT[GC]AC
1176 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI AocI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI TspRI XhoII