Restriction map for
pFN21AE4070
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstNI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRII EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvaI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 1102 GTGCAC G^TGCAC
ApaI 172 GGGCCC GGGCC^C
ApaLI 1102 GTGCAC G^TGCAC
AspHI 1102 GTGCAC G[AT]GC[AT]^C
AspI 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 733 CCCGGG C^[CT]CG[GA]G
BanI 13 GGTGCC G^G[CT][GA]CC
BbrPI 1105 CACGTG CAC^GTG
BglII 616 AGATCT A^GATCT
BsaAI 1105 CACGTG [CT]AC^GT[GA]
BsaI 602 GAGACC GGTCTC(1/5)
BsgI 1038 CTGCAC GTGCAG(16/14)
BsiHKAI 1102 GTGCAC G[AT]GC[AT]^C
BsoBI 733 CCCGGG C^[CT]CG[GA]G
BsrBI 937 GAGCGG CCGCTC(-3/-3)
BstAPI 1040 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1032 GGTTACC G^GT[ACGT]ACC
BstPI 1032 GGTTACC G^GT[ACGT]ACC
DraIII 1041 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1032 GGTTACC G^GT[ACGT]ACC
EcoRI 495 GAATTC G^AATTC
EcoT14I 743 CCAAGG C^C[AT][AT]GG
FauI 10 GCGGG CCCGC(4/6)
HaeII 335 AGCGCC [GA]GCGC^[CT]
HincII 844 GTCAAC GT[CT]^[GA]AC
HindII 844 GTCAAC GT[CT]^[GA]AC
HphI 360 GGTGA GGTGA(8/7)
MseI 346 TTAA T^TAA
NspI 228 GCATGT [GA]CATG^[CT]
PflFI 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1105 CACGTG CAC^GTG
PmlI 1105 CACGTG CAC^GTG
ScaI 441 AGTACT AGT^ACT
SmaI 733 CCCGGG CCC^GGG
StyI 743 CCAAGG C^C[AT][AT]GG
TaqI 1001 TCGA T^CGA
Tru9I 346 TTAA T^TAA
TspRI 764 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1003 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1001 TCGA T^CGA
XcmI 921 CCAAAAGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 733 CCCGGG C^CCGGG
XmaI 733 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 442 GTAC GT^AC
637 GTAC
AflIII 529 ACACGT A^C[GA][CT]GT
1106 ACGTGT
AgeI 109 ACCGGT A^CCGGT
893 ACCGGT
AlwI 435 GGATC GGATC(4/5)
723 GGATC
BanII 172 GGGCCC G[GA]GC[CT]^C
736 GGGCTC
BcnI 733 CCCGG CC^[GC]GG
734 CCGGG
BsaWI 109 ACCGGT [AT]^CCGG[AT]
893 ACCGGT
BsiYI 813 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1073 CCATCTGCCGG
BslI 813 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1073 CCATCTGCCGG
BsmAI 322 GAGAC GTCTC(1/5)
602 GAGAC
BsmFI 507 GTCCC GGGAC(10/14)
1070 GTCCC
BspMI 368 ACCTGC ACCTGC(4/8)
1036 ACCTGC
BssKI 733 CCCGG ^CC[ACGT]GG
734 CCGGG
BstYI 435 GGATCT [GA]^GATC[CT]
616 AGATCT
HaeIII 82 GGCC GG^CC
173 GGCC
MflI 435 GGATCT [GA]^GATC[CT]
616 AGATCT
MslI 18 CACGTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
981 CATCTGCATG
MspA1I 814 CAGCTG C[AC]G^C[GT]G
946 CAGCTG
NaeI 627 GCCGGC GCC^GGC
1079 GCCGGC
NciI 733 CCCGG CC^[GC]GG
734 CCGGG
NgoMIV 627 GCCGGC G^CCGGC
1079 GCCGGC
PinAI 109 ACCGGT A^CCGGT
893 ACCGGT
PpuMI 365 AGGACCT [GA]G^G[AT]CC[CT]
505 AGGTCCC
PstI 943 CTGCAG CTGCA^G
949 CTGCAG
PvuII 814 CAGCTG CAG^CTG
946 CAGCTG
RsaI 442 GTAC GT^AC
637 GTAC
ScrFI 733 CCCGG CC^[ACGT]GG
734 CCGGG
SfaNI 991 GATGC GCATC(5/9)
980 GCATC
SfcI 943 CTGCAG C^T[GA][CT]AG
949 CTGCAG
Tsp45I 239 GTGAC ^GT[GC]AC
308 GTCAC
XhoII 435 GGATCT [GA]^GATC[CT]
616 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI Bsp1286I BsrFI BsrI
BstF5I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I Fnu4HI
FokI HapII HhaI HinP1I HpaII
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MspI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I TseI
Tsp509I