Restriction map for
pFN21AE3219
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# NO CUT ENZYMES ( 115 enzymes) #
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Acc65I AccI AccII AfaI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1240 GACGTC GACGT^C
AccIII 1384 TCCGGA T^CCGGA
AclI 1363 AACGTT AA^CGTT
AcsI 135 GAATTT [GA]^AATT[CT]
Aor51HI 1169 AGCGCT AGC^GCT
ApaI 1014 GGGCCC GGGCC^C
ApoI 135 GAATTT [GA]^AATT[CT]
Asp700I 1167 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 295 GAGCTC G[AT]GC[AT]^C
BalI 1035 TGGCCA TGG^CCA
BamHI 1292 GGATCC G^GATCC
BbsI 368 GAAGAC GAAGAC(2/6)
BglI 1007 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1107 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1107 GCTAAGC GC^T[ACGT]AGC
BpuAI 368 GAAGAC GAAGAC(2/6)
BsaI 645 GAGACC GGTCTC(1/5)
BsaWI 1384 TCCGGA [AT]^CCGG[AT]
BseAI 1384 TCCGGA T^CCGGA
BsiHKAI 295 GAGCTC G[AT]GC[AT]^C
BsmBI 476 GAGACG CGTCTC(1/5)
BspEI 1384 TCCGGA T^CCGGA
BspHI 275 TCATGA T^CATGA
BspLU11I 1232 ACATGT A^CATGT
BspMI 350 ACCTGC ACCTGC(4/8)
BssSI 1264 CACGAG CACGAG(-5/-1)
BstAPI 549 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 628 GGTGACC G^GT[ACGT]ACC
BstPI 628 GGTGACC G^GT[ACGT]ACC
BstXI 944 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1107 GCTAAGC GC^T[ACGT]AGC
DraI 1315 TTTAAA TTT^AAA
DsaI 67 CCACGG C^C[GA][CT]GG
EaeI 1035 TGGCCA [CT]^GGCC[GA]
Eco47III 1169 AGCGCT AGC^GCT
EcoO65I 628 GGTGACC G^GT[ACGT]ACC
EcoT22I 514 ATGCAT ATGCA^T
HaeII 1169 AGCGCT [GA]GCGC^[CT]
HincII 391 GTCAAC GT[CT]^[GA]AC
HindII 391 GTCAAC GT[CT]^[GA]AC
HindIII 836 AAGCTT A^AGCTT
MluNI 1035 TGGCCA TGG^CCA
MroI 1384 TCCGGA T^CCGGA
MscI 1035 TGGCCA TGG^CCA
NsiI 514 ATGCAT ATGCA^T
Psp1406I 1363 AACGTT AA^CGTT
PstI 1329 CTGCAG CTGCA^G
RcaI 275 TCATGA T^CATGA
SacI 295 GAGCTC GAGCT^C
SapI 925 GAAGAGC GCTCTTC(1/4)
SfiI 1006 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 932 CTCAAG C^T[CT][GA]AG
StuI 1421 AGGCCT AGG^CCT
TaqI 434 TCGA T^CGA
TthHB8I 434 TCGA T^CGA
XcmI 650 CCAAGCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1167 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 78 GGCGTC G[GA]^CG[CT]C
1240 GACGTC
BanII 295 GAGCTC G[GA]GC[CT]^C
1014 GGGCCC
BbvCI 718 CCTCAGC CCTCAGC(-5/-2)
216 GCTGAGG
BmyI 295 GAGCTC G[AGT]GC[ACT]^C
1014 GGGCCC
BpmI 298 CTCCAG CTGGAG(16/14)
343 CTGGAG
BsaHI 78 GGCGTC G[GA]^CG[CT]C
1240 GACGTC
BsgI 569 CTGCAC GTGCAG(16/14)
325 GTGCAG
Bsp1286I 295 GAGCTC G[AGT]GC[ACT]^C
1014 GGGCCC
CfoI 1170 GCGC GCG^C
1374 GCGC
EarI 688 GAAGAG CTCTTC(1/4)
925 GAAGAG
EcoT14I 659 CCTTGG C^C[AT][AT]GG
1046 CCAAGG
HgaI 79 GCGTC GACGC(5/10)
1449 GCGTC
HhaI 1170 GCGC GCG^C
1374 GCGC
Hin1I 78 GGCGTC G[GA]^CG[CT]C
1240 GACGTC
HinP1I 1170 GCGC G^CGC
1374 GCGC
Ksp632I 688 GAAGAG CTCTTC(1/4)
925 GAAGAG
MseI 1091 TTAA T^TAA
1316 TTAA
PflMI 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCAGCCAGTGG
PpuMI 36 AGGACCC [GA]G^G[AT]CC[CT]
347 AGGACCT
PvuII 150 CAGCTG CAG^CTG
1432 CAGCTG
SfcI 386 CTATAG C^T[GA][CT]AG
1329 CTGCAG
StyI 659 CCTTGG C^C[AT][AT]GG
1046 CCAAGG
TfiI 873 GAATC G^A[AT]TC
964 GAATC
Tru9I 1091 TTAA T^TAA
1316 TTAA
Tsp509I 10 AATT ^AATT
136 AATT
Van91I 374 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCAGCCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AflIII AluI AlwI AlwNI
AvaII BbvI BcnI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XhoII