Restriction map for
pFN21AB5742
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AocI Aor51HI ApaI
AscI Asp718I AvaI AviII AvrII
BamHI BanI BbeI BcgI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2107 GTCTAC GT^[AC][GT]AC
AccIII 1997 TCCGGA T^CCGGA
AhdI 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2207 TGGCCA TGG^CCA
BbrPI 1196 CACGTG CAC^GTG
BbvCI 489 GCTGAGG CCTCAGC(-5/-2)
BciVI 1438 GGATAC GTATCC(6/5)
BfaI 1561 CTAG C^TAG
BglI 1274 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2057 GAGACC GGTCTC(1/5)
BsaWI 1997 TCCGGA [AT]^CCGG[AT]
BseAI 1997 TCCGGA T^CCGGA
BseRI 440 GAGGAG GAGGAG(10/8)
BsgI 763 GTGCAG GTGCAG(16/14)
BsmBI 1693 CGTCTC CGTCTC(1/5)
BspEI 1997 TCCGGA T^CCGGA
BspLU11I 1713 ACATGT A^CATGT
BspMI 1736 ACCTGC ACCTGC(4/8)
BsrBI 484 GAGCGG CCGCTC(-3/-3)
BstXI 2198 CCAGTGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1246 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 681 CCATGG C^C[GA][CT]GG
EaeI 2207 TGGCCA [CT]^GGCC[GA]
Eam1105I 1574 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 945 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 1998 CCGG C^CGG
HincII 112 GTTGAC GT[CT]^[GA]AC
HindII 112 GTTGAC GT[CT]^[GA]AC
HpaII 1998 CCGG C^CGG
MaeI 1561 CTAG C^TAG
MfeI 127 CAATTG C^AATTG
MluNI 2207 TGGCCA TGG^CCA
MroI 1997 TCCGGA T^CCGGA
MscI 2207 TGGCCA TGG^CCA
MslI 2014 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1998 CCGG C^CGG
MunI 127 CAATTG C^AATTG
NcoI 681 CCATGG C^CATGG
NspI 1713 ACATGT [GA]CATG^[CT]
PflFI 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1196 CACGTG CAC^GTG
PmlI 1196 CACGTG CAC^GTG
PshAI 35 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 62 CTGCAG CTGCA^G
SexAI 116 ACCTGGT A^CC[AT]GGT
SmlI 1081 CTCAAG C^T[CT][GA]AG
Tth111I 115 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1560 TCTAGA T^CTAGA
XcmI 506 CCACAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 199 GTAC GT^AC
893 GTAC
AflIII 537 ACACGT A^C[GA][CT]GT
1713 ACATGT
Alw44I 1199 GTGCAC G^TGCAC
1339 GTGCAC
AlwNI 1315 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1450 CAGGATCTG
ApaLI 1199 GTGCAC G^TGCAC
1339 GTGCAC
AseI 796 ATTAAT AT^TAAT
1906 ATTAAT
AsnI 796 ATTAAT AT^TAAT
1906 ATTAAT
BanII 633 GAGCTC G[GA]GC[CT]^C
1017 GAGCTC
BglII 1557 AGATCT A^GATCT
2091 AGATCT
BsaAI 538 CACGTA [CT]AC^GT[GA]
1196 CACGTG
BsmFI 33 GGGAC GGGAC(10/14)
1721 GGGAC
BstAPI 295 GCAGCGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
666 GCATCTGCTGC
Cac8I 834 GCTTGC GC[ACGT]^[ACGT]GC
2209 GCCAGC
EcoT14I 681 CCATGG C^C[AT][AT]GG
1569 CCTTGG
FauI 304 GCGGG CCCGC(4/6)
1206 GCGGG
HgaI 520 GACGC GACGC(5/10)
1782 GACGC
PleI 720 GACTC GAGTC(4/5)
1317 GACTC
PshBI 796 ATTAAT AT^TAAT
1906 ATTAAT
RsaI 199 GTAC GT^AC
893 GTAC
SacI 633 GAGCTC GAGCT^C
1017 GAGCTC
StyI 681 CCATGG C^C[AT][AT]GG
1569 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI