Restriction map for
pFN21AE3253
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# NO CUT ENZYMES ( 118 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 617 GACGTC GACGT^C
Acc65I 480 GGTACC G^GTACC
Alw44I 401 GTGCAC G^TGCAC
Aor51HI 425 AGCGCT AGC^GCT
ApaLI 401 GTGCAC G^TGCAC
Asp718I 480 GGTACC G^GTACC
AspI 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 698 CCCGGG C^[CT]CG[GA]G
AviII 384 TGCGCA TGC^GCA
BsiEI 657 CGATCG CG[GA][CT]^CG
BsoBI 698 CCCGGG C^[CT]CG[GA]G
BspDI 655 ATCGAT AT^CGAT
BssSI 409 CACGAG CACGAG(-5/-1)
ClaI 655 ATCGAT AT^CGAT
DrdI 547 GACTTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 425 AGCGCT AGC^GCT
EcoT14I 833 CCATGG C^C[AT][AT]GG
FspI 384 TGCGCA TGC^GCA
HindIII 463 AAGCTT A^AGCTT
KpnI 480 GGTACC GGTAC^C
MaeII 618 ACGT A^CGT
MseI 851 TTAA T^TAA
NcoI 833 CCATGG C^CATGG
PflFI 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 657 CGATCG CGAT^CG
SacI 623 GAGCTC GAGCT^C
SapI 107 GCTCTTC GCTCTTC(1/4)
SfiI 1126 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 698 CCCGGG CCC^GGG
SmlI 1182 CTTGAG C^T[CT][GA]AG
StuI 1125 AGGCCT AGG^CCT
StyI 833 CCATGG C^C[AT][AT]GG
TfiI 1060 GATTC G^A[AT]TC
Tru9I 851 TTAA T^TAA
Tth111I 1729 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 698 CCCGGG C^CCGGG
XmaI 698 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 22 AAATTT [GA]^AATT[CT]
847 GAATTT
AcyI 529 GACGCC G[GA]^CG[CT]C
617 GACGTC
ApoI 22 AAATTT [GA]^AATT[CT]
847 GAATTT
AspHI 401 GTGCAC G[AT]GC[AT]^C
623 GAGCTC
BalI 1001 TGGCCA TGG^CCA
1310 TGGCCA
BanII 224 GGGCTC G[GA]GC[CT]^C
623 GAGCTC
BbsI 271 GTCTTC GAAGAC(2/6)
1497 GTCTTC
BbvCI 1257 CCTCAGC CCTCAGC(-5/-2)
630 GCTGAGG
BpuAI 271 GTCTTC GAAGAC(2/6)
1497 GTCTTC
BsaHI 529 GACGCC G[GA]^CG[CT]C
617 GACGTC
BsaI 742 GAGACC GGTCTC(1/5)
54 GGTCTC
BsiHKAI 401 GTGCAC G[AT]GC[AT]^C
623 GAGCTC
BsmI 1368 GAATGC GAATGC(1/-1)
793 GCATTC
BspMI 745 ACCTGC ACCTGC(4/8)
565 GCAGGT
BsrFI 572 GCCGGT [GA]^CCGG[CT]
1761 GCCGGT
BstAPI 681 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
767 GCATCAAGTGC
Cfr10I 572 GCCGGT [GA]^CCGG[CT]
1761 GCCGGT
DsaI 315 CCGTGG C^C[GA][CT]GG
833 CCATGG
HaeII 236 AGCGCC [GA]GCGC^[CT]
425 AGCGCT
HgaI 529 GACGC GACGC(5/10)
1462 GACGC
Hin1I 529 GACGCC G[GA]^CG[CT]C
617 GACGTC
MluNI 1001 TGGCCA TGG^CCA
1310 TGGCCA
MscI 1001 TGGCCA TGG^CCA
1310 TGGCCA
MslI 300 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CACCTCAGTG
SpeI 1093 ACTAGT A^CTAGT
1291 ACTAGT
Tsp509I 23 AATT ^AATT
848 AATT
XcmI 833 CCATGGGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1323 CCACAGCTATTCTGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII