Restriction map for
pFN21AE4086
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AvaI AvaII AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AflIII 680 ACATGT A^C[GA][CT]GT
AhdI 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 334 GGGCCC GGGCC^C
AspEI 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 957 GGATCC G^GATCC
BbvCI 394 GCTGAGG CCTCAGC(-5/-2)
BsaBI 934 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 72 TCCGGT [AT]^CCGG[AT]
BsgI 162 GTGCAG GTGCAG(16/14)
BspLU11I 680 ACATGT A^CATGT
BspMI 14 ACCTGC ACCTGC(4/8)
BstAPI 104 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 75 GGTCACC G^GT[ACGT]ACC
BstPI 75 GGTCACC G^GT[ACGT]ACC
BstXI 379 CCAATAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 13 GACCTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 953 CCACGG C^C[GA][CT]GG
Eam1105I 505 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
MaeII 719 ACGT A^CGT
MamI 934 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 963 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Van91I 963 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1049 CCAACTGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 105 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
592 CAGGCACTG
Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAATGATTC
BbsI 22 GTCTTC GAAGAC(2/6)
219 GTCTTC
BpmI 730 CTCCAG CTGGAG(16/14)
824 CTGGAG
BpuAI 22 GTCTTC GAAGAC(2/6)
219 GTCTTC
BsmI 400 GAATGC GAATGC(1/-1)
1000 GCATTC
Bsp1407I 683 TGTACA T^GTACA
1342 TGTACA
BsrGI 683 TGTACA T^GTACA
1342 TGTACA
CfoI 150 GCGC GCG^C
994 GCGC
DraII 106 AGGCCCT [GA]G^G[ACGT]CC[CT]
334 GGGCCCT
EcoNI 900 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1136 CCTCAAAGAGG
EcoO109I 106 AGGCCCT [GA]G^G[ACGT]CC[CT]
334 GGGCCCT
HapII 73 CCGG C^CGG
1010 CCGG
HhaI 150 GCGC GCG^C
994 GCGC
HinP1I 150 GCGC G^CGC
994 GCGC
HpaII 73 CCGG C^CGG
1010 CCGG
MspI 73 CCGG C^CGG
1010 CCGG
NlaIV 334 GGGCCC GG[ACGT]^[ACGT]CC
957 GGATCC
PstI 589 CTGCAG CTGCA^G
1085 CTGCAG
SapI 613 GAAGAGC GCTCTTC(1/4)
300 GCTCTTC
SspBI 683 TGTACA T^GTACA
1342 TGTACA
StuI 1201 AGGCCT AGG^CCT
1321 AGGCCT
XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAATGATTC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AfaI AluI AlwI AspHI BanII
BbvI BfaI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII