Restriction map for
pFN21AE3290
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BbeI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
PacI PflFI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 575 GTAGAC GT^[AC][GT]AC
AgeI 1521 ACCGGT A^CCGGT
Alw44I 1130 GTGCAC G^TGCAC
AlwI 1222 GATCC GGATC(4/5)
ApaI 1243 GGGCCC GGGCC^C
ApaLI 1130 GTGCAC G^TGCAC
Asp700I 1972 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2111 TGGCCA TGG^CCA
BbrPI 1211 CACGTG CAC^GTG
BbsI 994 GAAGAC GAAGAC(2/6)
BcgI 2230 CGATTCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1963 CTAG C^TAG
BglII 358 AGATCT A^GATCT
BpuAI 994 GAAGAC GAAGAC(2/6)
BsaAI 1211 CACGTG [CT]AC^GT[GA]
BsaI 1094 GAGACC GGTCTC(1/5)
BsaWI 1521 ACCGGT [AT]^CCGG[AT]
BspDI 451 ATCGAT AT^CGAT
BsrBI 2008 GAGCGG CCGCTC(-3/-3)
BsrFI 1521 ACCGGT [GA]^CCGG[CT]
BstBI 1484 TTCGAA TT^CGAA
BstEII 759 GGTCACC G^GT[ACGT]ACC
BstPI 759 GGTCACC G^GT[ACGT]ACC
BstYI 358 AGATCT [GA]^GATC[CT]
Cfr10I 1521 ACCGGT [GA]^CCGG[CT]
ClaI 451 ATCGAT AT^CGAT
DraII 1946 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2111 TGGCCA [CT]^GGCC[GA]
EcoO109I 1946 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 759 GGTCACC G^GT[ACGT]ACC
EcoRV 1022 GATATC GAT^ATC
EcoT22I 548 ATGCAT ATGCA^T
HapII 1522 CCGG C^CGG
HpaII 1522 CCGG C^CGG
MaeI 1963 CTAG C^TAG
MflI 358 AGATCT [GA]^GATC[CT]
MluNI 2111 TGGCCA TGG^CCA
MscI 2111 TGGCCA TGG^CCA
MspI 1522 CCGG C^CGG
NdeI 968 CATATG CA^TATG
NsiI 548 ATGCAT ATGCA^T
NspV 1484 TTCGAA TT^CGAA
PinAI 1521 ACCGGT A^CCGGT
PleI 1104 GACTC GAGTC(4/5)
PmaCI 1211 CACGTG CAC^GTG
PmlI 1211 CACGTG CAC^GTG
PstI 1672 CTGCAG CTGCA^G
SacI 1177 GAGCTC GAGCT^C
SapI 2089 GAAGAGC GCTCTTC(1/4)
ScaI 625 AGTACT AGT^ACT
SfuI 1484 TTCGAA TT^CGAA
XhoII 358 AGATCT [GA]^GATC[CT]
XmnI 1972 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 1560 GGTGCC G^G[CT][GA]CC
1686 GGTGCC
BsmFI 927 GTCCC GGGAC(10/14)
1447 GTCCC
BsmI 546 GAATGC GAATGC(1/-1)
1487 GAATGC
DpnII 359 GATC ^GATC
1222 GATC
EcoRI 700 GAATTC G^AATTC
1481 GAATTC
HincII 229 GTTGAC GT[CT]^[GA]AC
772 GTTGAC
HindII 229 GTTGAC GT[CT]^[GA]AC
772 GTTGAC
MaeII 1212 ACGT A^CGT
1638 ACGT
MboI 359 GATC ^GATC
1222 GATC
MspA1I 1831 CAGCTG C[AC]G^C[GT]G
1981 CAGCTG
NdeII 359 GATC ^GATC
1222 GATC
NlaIII 98 CATG CATG^
409 CATG
PaeR7I 1180 CTCGAG C^TCGAG
1625 CTCGAG
PvuII 1831 CAGCTG CAG^CTG
1981 CAGCTG
Sau3AI 359 GATC ^GATC
1222 GATC
SfcI 1672 CTGCAG C^T[GA][CT]AG
2080 CTACAG
SmlI 1180 CTCGAG C^T[CT][GA]AG
1625 CTCGAG
XhoI 1180 CTCGAG C^TCGAG
1625 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaI BanII BbvCI
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboII MnlI MseI MvaI MwoI
NlaIV PflMI RsaI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I