Restriction map for
pFN21AE3265
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccII AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 1889 TCCGGA T^CCGGA
AclI 1529 AACGTT AA^CGTT
AlwNI 944 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1336 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1055 GAGCAC G[AT]GC[AT]^C
AvaI 1052 CTCGAG C^[CT]CG[GA]G
BamHI 844 GGATCC G^GATCC
BanI 1575 GGTGCC G^G[CT][GA]CC
BanII 507 GAGCCC G[GA]GC[CT]^C
BbvCI 1783 CCTCAGC CCTCAGC(-5/-2)
BpmI 35 CTGGAG CTGGAG(16/14)
BsaWI 1889 TCCGGA [AT]^CCGG[AT]
BseAI 1889 TCCGGA T^CCGGA
BsgI 191 GTGCAG GTGCAG(16/14)
BsiHKAI 1055 GAGCAC G[AT]GC[AT]^C
BsmFI 795 GTCCC GGGAC(10/14)
BsoBI 1052 CTCGAG C^[CT]CG[GA]G
BspEI 1889 TCCGGA T^CCGGA
BsrBI 1129 GAGCGG CCGCTC(-3/-3)
BssSI 1058 CACGAG CACGAG(-5/-1)
BstAPI 1872 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1580 CCATTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1071 GCGC GCG^C
DraI 703 TTTAAA TTT^AAA
DsaI 1652 CCATGG C^C[GA][CT]GG
EcoT14I 1652 CCATGG C^C[AT][AT]GG
EcoT22I 1472 ATGCAT ATGCA^T
HapII 1890 CCGG C^CGG
HhaI 1071 GCGC GCG^C
HinP1I 1071 GCGC G^CGC
HincII 1561 GTTGAC GT[CT]^[GA]AC
HindII 1561 GTTGAC GT[CT]^[GA]AC
HpaII 1890 CCGG C^CGG
MaeII 1530 ACGT A^CGT
MroI 1889 TCCGGA T^CCGGA
MspI 1890 CCGG C^CGG
NcoI 1652 CCATGG C^CATGG
NdeI 434 CATATG CA^TATG
NsiI 1472 ATGCAT ATGCA^T
PaeR7I 1052 CTCGAG C^TCGAG
PflMI 1742 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1529 AACGTT AA^CGTT
PstI 1677 CTGCAG CTGCA^G
SpeI 1036 ACTAGT A^CTAGT
SphI 1470 GCATGC GCATG^C
SspI 1633 AATATT AAT^ATT
StyI 1652 CCATGG C^C[AT][AT]GG
Van91I 1742 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1052 CTCGAG C^TCGAG
XmnI 1336 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 297 AAATTC [GA]^AATT[CT]
1255 GAATTT
AflIII 175 ACATGT A^C[GA][CT]GT
531 ACATGT
ApoI 297 AAATTC [GA]^AATT[CT]
1255 GAATTT
AvaII 108 GGACC G^G[AT]CC
162 GGACC
BalI 270 TGGCCA TGG^CCA
285 TGGCCA
BfaI 1037 CTAG C^TAG
1606 CTAG
BsaJI 1587 CCTGGG C^C[ACGT][ACGT]GG
1652 CCATGG
BsmAI 550 GAGAC GTCTC(1/5)
657 GTCTC
BspLU11I 175 ACATGT A^CATGT
531 ACATGT
Cfr13I 108 GGACC G^G[ACGT]CC
162 GGACC
EaeI 270 TGGCCA [CT]^GGCC[GA]
285 TGGCCA
EarI 863 CTCTTC CTCTTC(1/4)
547 GAAGAG
FauI 400 GCGGG CCCGC(4/6)
1131 GCGGG
HphI 882 TCACC GGTGA(8/7)
1584 TCACC
Ksp632I 863 CTCTTC CTCTTC(1/4)
547 GAAGAG
MaeI 1037 CTAG C^TAG
1606 CTAG
MluNI 270 TGGCCA TGG^CCA
285 TGGCCA
MscI 270 TGGCCA TGG^CCA
285 TGGCCA
MspA1I 586 CAGCTG C[AC]G^C[GT]G
790 CAGCTG
NlaIV 844 GGATCC GG[ACGT]^[ACGT]CC
1575 GGTGCC
PvuII 586 CAGCTG CAG^CTG
790 CAGCTG
Sau96I 108 GGACC G^G[ACGT]CC
162 GGACC
SfaNI 31 GATGC GCATC(5/9)
724 GCATC
SmlI 375 CTCAAG C^T[CT][GA]AG
1052 CTCGAG
XcmI 273 CCAGAGTGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
466 CCAAGTCCATCTTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NspI
PleI RsaI Sau3AI ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII