################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 1889 TCCGGA T^CCGGA AclI 1529 AACGTT AA^CGTT AlwNI 944 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1336 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1055 GAGCAC G[AT]GC[AT]^C AvaI 1052 CTCGAG C^[CT]CG[GA]G BamHI 844 GGATCC G^GATCC BanI 1575 GGTGCC G^G[CT][GA]CC BanII 507 GAGCCC G[GA]GC[CT]^C BbvCI 1783 CCTCAGC CCTCAGC(-5/-2) BpmI 35 CTGGAG CTGGAG(16/14) BsaWI 1889 TCCGGA [AT]^CCGG[AT] BseAI 1889 TCCGGA T^CCGGA BsgI 191 GTGCAG GTGCAG(16/14) BsiHKAI 1055 GAGCAC G[AT]GC[AT]^C BsmFI 795 GTCCC GGGAC(10/14) BsoBI 1052 CTCGAG C^[CT]CG[GA]G BspEI 1889 TCCGGA T^CCGGA BsrBI 1129 GAGCGG CCGCTC(-3/-3) BssSI 1058 CACGAG CACGAG(-5/-1) BstAPI 1872 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1580 CCATTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1071 GCGC GCG^C DraI 703 TTTAAA TTT^AAA DsaI 1652 CCATGG C^C[GA][CT]GG EcoT14I 1652 CCATGG C^C[AT][AT]GG EcoT22I 1472 ATGCAT ATGCA^T HapII 1890 CCGG C^CGG HhaI 1071 GCGC GCG^C HinP1I 1071 GCGC G^CGC HincII 1561 GTTGAC GT[CT]^[GA]AC HindII 1561 GTTGAC GT[CT]^[GA]AC HpaII 1890 CCGG C^CGG MaeII 1530 ACGT A^CGT MroI 1889 TCCGGA T^CCGGA MspI 1890 CCGG C^CGG NcoI 1652 CCATGG C^CATGG NdeI 434 CATATG CA^TATG NsiI 1472 ATGCAT ATGCA^T PaeR7I 1052 CTCGAG C^TCGAG PflMI 1742 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1529 AACGTT AA^CGTT PstI 1677 CTGCAG CTGCA^G SpeI 1036 ACTAGT A^CTAGT SphI 1470 GCATGC GCATG^C SspI 1633 AATATT AAT^ATT StyI 1652 CCATGG C^C[AT][AT]GG Van91I 1742 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1052 CTCGAG C^TCGAG XmnI 1336 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 297 AAATTC [GA]^AATT[CT] 1255 GAATTT AflIII 175 ACATGT A^C[GA][CT]GT 531 ACATGT ApoI 297 AAATTC [GA]^AATT[CT] 1255 GAATTT AvaII 108 GGACC G^G[AT]CC 162 GGACC BalI 270 TGGCCA TGG^CCA 285 TGGCCA BfaI 1037 CTAG C^TAG 1606 CTAG BsaJI 1587 CCTGGG C^C[ACGT][ACGT]GG 1652 CCATGG BsmAI 550 GAGAC GTCTC(1/5) 657 GTCTC BspLU11I 175 ACATGT A^CATGT 531 ACATGT Cfr13I 108 GGACC G^G[ACGT]CC 162 GGACC EaeI 270 TGGCCA [CT]^GGCC[GA] 285 TGGCCA EarI 863 CTCTTC CTCTTC(1/4) 547 GAAGAG FauI 400 GCGGG CCCGC(4/6) 1131 GCGGG HphI 882 TCACC GGTGA(8/7) 1584 TCACC Ksp632I 863 CTCTTC CTCTTC(1/4) 547 GAAGAG MaeI 1037 CTAG C^TAG 1606 CTAG MluNI 270 TGGCCA TGG^CCA 285 TGGCCA MscI 270 TGGCCA TGG^CCA 285 TGGCCA MspA1I 586 CAGCTG C[AC]G^C[GT]G 790 CAGCTG NlaIV 844 GGATCC GG[ACGT]^[ACGT]CC 1575 GGTGCC PvuII 586 CAGCTG CAG^CTG 790 CAGCTG Sau96I 108 GGACC G^G[ACGT]CC 162 GGACC SfaNI 31 GATGC GCATC(5/9) 724 GCATC SmlI 375 CTCAAG C^T[CT][GA]AG 1052 CTCGAG XcmI 273 CCAGAGTGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 466 CCAAGTCCATCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII