Restriction map for
pFN21AB7989
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III EcoNI EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 145 GAATTC [GA]^AATT[CT]
AocI 286 CCTCAGG CC^T[ACGT]AGG
ApaI 62 GGGCCC GGGCC^C
ApoI 145 GAATTC [GA]^AATT[CT]
BalI 605 TGGCCA TGG^CCA
BbsI 649 GTCTTC GAAGAC(2/6)
BclI 551 TGATCA T^GATCA
BglII 1049 AGATCT A^GATCT
BlpI 681 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC
BpuAI 649 GTCTTC GAAGAC(2/6)
BsmFI 242 GGGAC GGGAC(10/14)
BsrDI 511 GCAATG GCAATG(2/0)
BsrI 543 CCAGT ACTGG(1/-1)
BssHII 437 GCGCGC G^CGCGC
BssSI 328 CACGAG CACGAG(-5/-1)
BstYI 1049 AGATCT [GA]^GATC[CT]
Bsu36I 286 CCTCAGG CC^T[ACGT]AGG
CelII 681 GCTCAGC GC^T[ACGT]AGC
Eco81I 286 CCTCAGG CC^T[ACGT]AGG
EcoRI 145 GAATTC G^AATTC
FbaI 551 TGATCA T^GATCA
MflI 1049 AGATCT [GA]^GATC[CT]
MluNI 605 TGGCCA TGG^CCA
MscI 605 TGGCCA TGG^CCA
MslI 399 CACTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 248 CATATG CA^TATG
NspI 1028 GCATGC [GA]CATG^[CT]
PstI 505 CTGCAG CTGCA^G
SbfI 504 CCTGCAGG CCTGCA^GG
SfcI 505 CTGCAG C^T[GA][CT]AG
SfiI 291 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1002 CCCGGG CCC^GGG
SmlI 1237 CTCAAG C^T[CT][GA]AG
SphI 1028 GCATGC GCATG^C
Sse8387I 504 CCTGCAGG CCTGCA^GG
Tsp509I 146 AATT ^AATT
XhoII 1049 AGATCT [GA]^GATC[CT]
XmaCI 1002 CCCGGG C^CCGGG
XmaI 1002 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 7 GTGCAC G^TGCAC
913 GTGCAC
ApaLI 7 GTGCAC G^TGCAC
913 GTGCAC
AspHI 7 GTGCAC G[AT]GC[AT]^C
913 GTGCAC
BfaI 538 CTAG C^TAG
895 CTAG
BseRI 284 CTCCTC GAGGAG(10/8)
331 GAGGAG
BsiEI 125 CGGCCG CG[GA][CT]^CG
666 CGGCCG
BsiHKAI 7 GTGCAC G[AT]GC[AT]^C
913 GTGCAC
BsmBI 74 CGTCTC CGTCTC(1/5)
946 GAGACG
BspMI 503 ACCTGC ACCTGC(4/8)
845 ACCTGC
BsrBI 109 CCGCTC CCGCTC(-3/-3)
1086 CCGCTC
DpnII 552 GATC ^GATC
1050 GATC
DsaI 23 CCATGG C^C[GA][CT]GG
1170 CCATGG
EagI 125 CGGCCG C^GGCCG
666 CGGCCG
EclXI 125 CGGCCG C^GGCCG
666 CGGCCG
Eco52I 125 CGGCCG C^GGCCG
666 CGGCCG
HinfI 731 GACTC G^A[ACGT]TC
1231 GAGTC
MaeI 538 CTAG C^TAG
895 CTAG
MaeII 691 ACGT A^CGT
938 ACGT
MboI 552 GATC ^GATC
1050 GATC
MboII 831 GAAGA GAAGA(8/7)
650 TCTTC
NcoI 23 CCATGG C^CATGG
1170 CCATGG
NdeII 552 GATC ^GATC
1050 GATC
PleI 731 GACTC GAGTC(4/5)
1231 GAGTC
PpuMI 242 GGGACCC [GA]G^G[AT]CC[CT]
500 AGGACCT
Sau3AI 552 GATC ^GATC
1050 GATC
XcmI 608 CCAAAGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1170 CCATGGCTTCGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MaeIII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIII NlaIV
PvuII RsaI Sau96I ScrFI SfaNI
SfoI StyI TseI Tsp45I TspRI