Restriction map for
pFN21AE4258
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccII AclI AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspLU11I BsrBI BsrFI BssHII
BssSI BstAPI BstUI BstXI CelII
Cfr10I CpoI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SphI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 2915 GGTACC G^GTACC
AccIII 1463 TCCGGA T^CCGGA
AhdI 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 837 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2915 GGTACC G^GTACC
AspEI 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2915 GGTACC G^G[CT][GA]CC
BglII 346 AGATCT A^GATCT
BsaAI 2150 TACGTA [CT]AC^GT[GA]
BsaI 1788 GAGACC GGTCTC(1/5)
BseAI 1463 TCCGGA T^CCGGA
BseRI 282 GAGGAG GAGGAG(10/8)
BsgI 206 GTGCAG GTGCAG(16/14)
BsmFI 1584 GGGAC GGGAC(10/14)
BsmI 2177 GAATGC GAATGC(1/-1)
Bsp1407I 2759 TGTACA T^GTACA
BspDI 1550 ATCGAT AT^CGAT
BspEI 1463 TCCGGA T^CCGGA
BspHI 1344 TCATGA T^CATGA
BsrDI 1333 GCAATG GCAATG(2/0)
BsrGI 2759 TGTACA T^GTACA
Bst1107I 17 GTATAC GTA^TAC
BstBI 299 TTCGAA TT^CGAA
BstEII 2231 GGTTACC G^GT[ACGT]ACC
BstPI 2231 GGTTACC G^GT[ACGT]ACC
BstZ17I 17 GTATAC GTA^TAC
CfoI 1521 GCGC GCG^C
ClaI 1550 ATCGAT AT^CGAT
DraI 979 TTTAAA TTT^AAA
DraII 419 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2788 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 419 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2231 GGTTACC G^GT[ACGT]ACC
EcoRI 2539 GAATTC G^AATTC
EcoT22I 310 ATGCAT ATGCA^T
FauI 1353 CCCGC CCCGC(4/6)
HaeII 1520 GGCGCT [GA]GCGC^[CT]
HhaI 1521 GCGC GCG^C
HinP1I 1521 GCGC G^CGC
KpnI 2915 GGTACC GGTAC^C
MaeII 2151 ACGT A^CGT
MroI 1463 TCCGGA T^CCGGA
MslI 2172 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 310 ATGCAT ATGCA^T
NspV 299 TTCGAA TT^CGAA
PflFI 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1681 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2767 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2660 CTGCAG CTGCA^G
RcaI 1344 TCATGA T^CATGA
ScaI 2439 AGTACT AGT^ACT
SfuI 299 TTCGAA TT^CGAA
SnaBI 2150 TACGTA TAC^GTA
SpeI 1089 ACTAGT A^CTAGT
SspBI 2759 TGTACA T^GTACA
SspI 1693 AATATT AAT^ATT
StuI 2397 AGGCCT AGG^CCT
Tth111I 2059 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1681 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 837 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 1358 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2855 CAGCCACTG
AocI 1719 CCTGAGG CC^T[ACGT]AGG
2867 CCTTAGG
BcnI 202 CCCGG CC^[GC]GG
1582 CCGGG
BsaWI 696 TCCGGT [AT]^CCGG[AT]
1463 TCCGGA
Bsu36I 1719 CCTGAGG CC^T[ACGT]AGG
2867 CCTTAGG
Cac8I 262 GCAAGC GC[ACGT]^[ACGT]GC
2290 GCAAGC
Eco81I 1719 CCTGAGG CC^T[ACGT]AGG
2867 CCTTAGG
EcoNI 364 CCTTACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1715 CCTCCCTGAGG
EcoT14I 141 CCTTGG C^C[AT][AT]GG
2295 CCTTGG
HgaI 1360 GACGC GACGC(5/10)
2772 GCGTC
NciI 202 CCCGG CC^[GC]GG
1582 CCGGG
NspI 176 ACATGC [GA]CATG^[CT]
500 ACATGC
StyI 141 CCTTGG C^C[AT][AT]GG
2295 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII