Restriction map for
pFN21AE4194
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BlnI BsaHI BsiEI BsiWI
BsmI BsoBI BspDI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1654 GTAGAC GT^[AC][GT]AC
AccIII 48 TCCGGA T^CCGGA
AflII 403 CTTAAG C^TTAAG
ApaI 2060 GGGCCC GGGCC^C
Asp700I 1937 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 202 GAGCAC G[AT]GC[AT]^C
BanI 413 GGTGCC G^G[CT][GA]CC
BanII 2060 GGGCCC G[GA]GC[CT]^C
BbsI 1941 GTCTTC GAAGAC(2/6)
BbvCI 242 CCTCAGC CCTCAGC(-5/-2)
BclI 570 TGATCA T^GATCA
BfrI 403 CTTAAG C^TTAAG
BglI 1042 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1055 AGATCT A^GATCT
BlpI 1281 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1281 GCTGAGC GC^T[ACGT]AGC
BpuAI 1941 GTCTTC GAAGAC(2/6)
BsaAI 1751 CACGTA [CT]AC^GT[GA]
BsaBI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 48 TCCGGA [AT]^CCGG[AT]
BseAI 48 TCCGGA T^CCGGA
BsiHKAI 202 GAGCAC G[AT]GC[AT]^C
BsmBI 1192 GAGACG CGTCTC(1/5)
Bsp1407I 612 TGTACA T^GTACA
BspEI 48 TCCGGA T^CCGGA
BsrDI 606 CATTGC GCAATG(2/0)
BsrGI 612 TGTACA T^GTACA
CelII 1281 GCTGAGC GC^T[ACGT]AGC
CfoI 250 GCGC GCG^C
DraII 725 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 253 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1597 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 725 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 570 TGATCA T^GATCA
HgaI 7 GCGTC GACGC(5/10)
HhaI 250 GCGC GCG^C
HinP1I 250 GCGC G^CGC
HincII 1146 GTCAAC GT[CT]^[GA]AC
HindII 1146 GTCAAC GT[CT]^[GA]AC
MaeII 1752 ACGT A^CGT
MamI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 48 TCCGGA T^CCGGA
MslI 1419 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 632 CAGCTG C[AC]G^C[GT]G
PflMI 529 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 725 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 632 CAGCTG CAG^CTG
SapI 581 GCTCTTC GCTCTTC(1/4)
ScaI 1837 AGTACT AGT^ACT
SexAI 254 ACCAGGT A^CC[AT]GGT
SfcI 518 CTGTAG C^T[GA][CT]AG
SspBI 612 TGTACA T^GTACA
StuI 1767 AGGCCT AGG^CCT
Van91I 529 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1364 CCAGTCCCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1937 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 672 GAATTT [GA]^AATT[CT]
2053 AAATTC
AlwNI 5 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
913 CAGGATCTG
ApoI 672 GAATTT [GA]^AATT[CT]
2053 AAATTC
AvaII 726 GGTCC G^G[AT]CC
1564 GGTCC
BalI 29 TGGCCA TGG^CCA
335 TGGCCA
BmyI 202 GAGCAC G[AGT]GC[ACT]^C
2060 GGGCCC
BseRI 1379 CTCCTC GAGGAG(10/8)
352 GAGGAG
Bsp1286I 202 GAGCAC G[AGT]GC[ACT]^C
2060 GGGCCC
BspMI 232 GCAGGT ACCTGC(4/8)
1404 GCAGGT
BsrBI 1443 CCGCTC CCGCTC(-3/-3)
1822 GAGCGG
EaeI 29 TGGCCA [CT]^GGCC[GA]
335 TGGCCA
MluNI 29 TGGCCA TGG^CCA
335 TGGCCA
MscI 29 TGGCCA TGG^CCA
335 TGGCCA
NspI 1571 ACATGC [GA]CATG^[CT]
1947 ACATGC
PshAI 82 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2080 GACACTTGTC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BfaI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII