Restriction map for
pFN21AE1829
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MluI
MroI MseI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 707 GTCGAC GT^[AC][GT]AC
AclI 902 AACGTT AA^CGTT
AlwNI 889 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 71 TGGCCA TGG^CCA
BbsI 948 GAAGAC GAAGAC(2/6)
BfaI 997 CTAG C^TAG
BglI 34 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 948 GAAGAC GAAGAC(2/6)
BseRI 856 GAGGAG GAGGAG(10/8)
BsgI 28 CTGCAC GTGCAG(16/14)
BsiWI 88 CGTACG C^GTACG
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BstXI 293 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 258 GGATCT [GA]^GATC[CT]
DsaI 544 CCGCGG C^C[GA][CT]GG
EcoT14I 440 CCTTGG C^C[AT][AT]GG
EcoT22I 878 ATGCAT ATGCA^T
HaeII 491 AGCGCC [GA]GCGC^[CT]
HapII 279 CCGG C^CGG
HpaII 279 CCGG C^CGG
KspI 544 CCGCGG CCGC^GG
MaeI 997 CTAG C^TAG
MflI 258 GGATCT [GA]^GATC[CT]
MluNI 71 TGGCCA TGG^CCA
MscI 71 TGGCCA TGG^CCA
MspI 279 CCGG C^CGG
NsiI 878 ATGCAT ATGCA^T
Psp1406I 902 AACGTT AA^CGTT
PstI 283 CTGCAG CTGCA^G
SacII 544 CCGCGG CCGC^GG
SalI 707 GTCGAC G^TCGAC
SphI 965 GCATGC GCATG^C
StuI 469 AGGCCT AGG^CCT
StyI 440 CCTTGG C^C[AT][AT]GG
TaqI 708 TCGA T^CGA
TthHB8I 708 TCGA T^CGA
XhoII 258 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 258 GGATC GGATC(4/5)
791 GGATC
BanI 701 GGCACC G^G[CT][GA]CC
1034 GGTGCC
BsrI 215 ACTGG ACTGG(1/-1)
943 CCAGT
BstEII 429 GGTCACC G^GT[ACGT]ACC
462 GGTGACC
BstPI 429 GGTCACC G^GT[ACGT]ACC
462 GGTGACC
CfoI 79 GCGC GCG^C
492 GCGC
EaeI 71 TGGCCA [CT]^GGCC[GA]
541 TGGCCG
EcoNI 45 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
597 CCTCTCGCAGG
EcoO65I 429 GGTCACC G^GT[ACGT]ACC
462 GGTGACC
HhaI 79 GCGC GCG^C
492 GCGC
HinP1I 79 GCGC G^CGC
492 GCGC
HincII 707 GTCGAC GT[CT]^[GA]AC
731 GTCAAC
HindII 707 GTCGAC GT[CT]^[GA]AC
731 GTCAAC
HinfI 891 GAGTC G^A[ACGT]TC
920 GAGTC
MaeII 220 ACGT A^CGT
903 ACGT
MslI 567 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1014 CATGATCGTG
NspI 128 ACATGC [GA]CATG^[CT]
965 GCATGC
PleI 891 GAGTC GAGTC(4/5)
920 GAGTC
PshAI 674 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
724 GACAGAAGTC
SacI 274 GAGCTC GAGCT^C
651 GAGCTC
SfcI 181 CTACAG C^T[GA][CT]AG
283 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AspHI AvaII BanII BbvI BlpI
BmyI BpmI Bpu1102I BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CelII
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI