Restriction map for
pFN21AE3136
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I DraI
DraII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI FseI
FspI HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI SacI SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 99 CGCG CG^CG
AlwNI 181 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbeI 42 GGCGCC GGCGC^C
BclI 353 TGATCA T^GATCA
BmyI 533 GTGCCC G[AGT]GC[ACT]^C
BpmI 214 CTCCAG CTGGAG(16/14)
Bsp1286I 533 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 882 TGTACA T^GTACA
BspDI 675 ATCGAT AT^CGAT
BspMI 390 ACCTGC ACCTGC(4/8)
BsrDI 302 GCAATG GCAATG(2/0)
BsrGI 882 TGTACA T^GTACA
BstUI 99 CGCG CG^CG
BstXI 449 CCAGAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 294 GGATCT [GA]^GATC[CT]
CfoI 43 GCGC GCG^C
ClaI 675 ATCGAT AT^CGAT
CpoI 124 CGGACCG CG^G[AT]CCG
DraIII 132 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 672 GATATC GAT^ATC
EcoT22I 957 ATGCAT ATGCA^T
FbaI 353 TGATCA T^GATCA
HaeII 42 GGCGCC [GA]GCGC^[CT]
HhaI 43 GCGC GCG^C
HinP1I 43 GCGC G^CGC
HincII 937 GTCAAC GT[CT]^[GA]AC
HindII 937 GTCAAC GT[CT]^[GA]AC
KasI 42 GGCGCC G^GCGCC
KspI 98 CCGCGG CCGC^GG
MflI 294 GGATCT [GA]^GATC[CT]
MseI 979 TTAA T^TAA
MvnI 99 CGCG CG^CG
NarI 42 GGCGCC GG^CGCC
NsiI 957 ATGCAT ATGCA^T
RsrII 124 CGGACCG CG^G[AT]CCG
SacII 98 CCGCGG CCGC^GG
ScaI 623 AGTACT AGT^ACT
SfcI 588 CTATAG C^T[GA][CT]AG
SfoI 42 GGCGCC GGC^GCC
SmaI 200 CCCGGG CCC^GGG
SpeI 487 ACTAGT A^CTAGT
SspBI 882 TGTACA T^GTACA
Tru9I 979 TTAA T^TAA
TspRI 890 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 294 GGATCT [GA]^GATC[CT]
XmaCI 200 CCCGGG C^CCGGG
XmaI 200 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 200 CCCGGG C^[CT]CG[GA]G
283 CCCGAG
BbsI 322 GAAGAC GAAGAC(2/6)
364 GAAGAC
BpuAI 322 GAAGAC GAAGAC(2/6)
364 GAAGAC
BsmBI 63 CGTCTC CGTCTC(1/5)
546 GAGACG
BsmFI 82 GGGAC GGGAC(10/14)
460 GTCCC
BsoBI 200 CCCGGG C^[CT]CG[GA]G
283 CCCGAG
Cac8I 91 GCTGGC GC[ACGT]^[ACGT]GC
571 GCAAGC
DsaI 98 CCGCGG C^C[GA][CT]GG
455 CCGTGG
EcoT14I 158 CCAAGG C^C[AT][AT]GG
1010 CCAAGG
HgaI 548 GACGC GACGC(5/10)
62 GCGTC
MaeII 405 ACGT A^CGT
422 ACGT
MspA1I 98 CCGCGG C[AC]G^C[GT]G
147 CCGCTG
MwoI 91 GCTGGCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
901 GCAGATGAAGC
NspI 347 ACATGC [GA]CATG^[CT]
1005 ACATGC
PleI 32 GAGTC GAGTC(4/5)
515 GAGTC
StyI 158 CCAAGG C^C[AT][AT]GG
1010 CCAAGG
TaqI 506 TCGA T^CGA
676 TCGA
TfiI 263 GAATC G^A[AT]TC
522 GAATC
Tsp45I 344 GTGAC ^GT[GC]AC
629 GTGAC
TthHB8I 506 TCGA T^CGA
676 TCGA
XcmI 36 CCACCCGGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1023 CCATACCAACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BbvI BciVI BcnI
BfaI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
Hin1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspI MvaI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp509I