Restriction map for
pFN21AE3307
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AhdI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MluI MluNI
MscI NarI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 926 TCCGGA T^CCGGA
AcsI 1810 GAATTT [GA]^AATT[CT]
AgeI 2134 ACCGGT A^CCGGT
Alw44I 1684 GTGCAC G^TGCAC
AlwNI 1108 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 296 AGCGCT AGC^GCT
ApaLI 1684 GTGCAC G^TGCAC
ApoI 1810 GAATTT [GA]^AATT[CT]
AspI 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 568 GGCACC G^G[CT][GA]CC
BbsI 1857 GTCTTC GAAGAC(2/6)
BclI 1509 TGATCA T^GATCA
BfaI 1001 CTAG C^TAG
BlpI 1329 GCTAAGC GC^T[ACGT]AGC
BpmI 991 CTGGAG CTGGAG(16/14)
Bpu1102I 1329 GCTAAGC GC^T[ACGT]AGC
BpuAI 1857 GTCTTC GAAGAC(2/6)
BsaBI 1605 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 926 TCCGGA T^CCGGA
BsgI 375 GTGCAG GTGCAG(16/14)
BspEI 926 TCCGGA T^CCGGA
BstAPI 1369 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 292 CCAAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1329 GCTAAGC GC^T[ACGT]AGC
DraIII 383 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2114 CCATGG C^C[GA][CT]GG
Eco47III 296 AGCGCT AGC^GCT
FbaI 1509 TGATCA T^GATCA
HaeII 296 AGCGCT [GA]GCGC^[CT]
MaeI 1001 CTAG C^TAG
MamI 1605 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1502 CAATTG C^AATTG
MroI 926 TCCGGA T^CCGGA
MslI 690 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1502 CAATTG C^AATTG
NaeI 29 GCCGGC GCC^GGC
NcoI 2114 CCATGG C^CATGG
NdeI 44 CATATG CA^TATG
NgoMIV 29 GCCGGC G^CCGGC
PflFI 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1077 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2134 ACCGGT A^CCGGT
PshAI 892 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1206 CTGCAG CTGCA^G
PvuI 563 CGATCG CGAT^CG
PvuII 2031 CAGCTG CAG^CTG
TaqI 562 TCGA T^CGA
Tth111I 550 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 562 TCGA T^CGA
Van91I 1077 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 151 GACGCC G[GA]^CG[CT]C
341 GACGCC
AocI 720 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
Asp700I 1088 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1489 GAAGTGTTTC
BamHI 1240 GGATCC G^GATCC
1567 GGATCC
BanII 311 GAGCTC G[GA]GC[CT]^C
356 GAGCTC
BsaHI 151 GACGCC G[GA]^CG[CT]C
341 GACGCC
BspHI 1274 TCATGA T^CATGA
2171 TCATGA
BsrBI 394 CCGCTC CCGCTC(-3/-3)
606 GAGCGG
BsrDI 1429 CATTGC GCAATG(2/0)
1536 CATTGC
Bsu36I 720 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
DraI 1066 TTTAAA TTT^AAA
1780 TTTAAA
DrdI 839 GACCAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1188 GACTGATGTGTC
Eco81I 720 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
HgaI 151 GACGC GACGC(5/10)
341 GACGC
Hin1I 151 GACGCC G[GA]^CG[CT]C
341 GACGCC
MaeII 1961 ACGT A^CGT
2108 ACGT
RcaI 1274 TCATGA T^CATGA
2171 TCATGA
SacI 311 GAGCTC GAGCT^C
356 GAGCTC
SfcI 1206 CTGCAG C^T[GA][CT]AG
2163 CTACAG
SmaI 15 CCCGGG CCC^GGG
529 CCCGGG
TfiI 766 GATTC G^A[AT]TC
1093 GATTC
XmaCI 15 CCCGGG C^CCGGG
529 CCCGGG
XmaI 15 CCCGGG C^CCGGG
529 CCCGGG
XmnI 1088 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1489 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BbvCI BbvI BcnI
BmyI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII