Restriction map for
pFN21AE4002
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseRI BsgI BsiEI BsiWI BsoBI
BspDI BspLU11I BsrBI BsrDI BsrFI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluNI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 271 GTCTAC GT^[AC][GT]AC
AccIII 245 TCCGGA T^CCGGA
AcyI 360 GACGCC G[GA]^CG[CT]C
AlwNI 325 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 390 GGACC G^G[AT]CC
BcnI 15 CCGGG CC^[GC]GG
BsaHI 360 GACGCC G[GA]^CG[CT]C
BsaI 3 GGTCTC GGTCTC(1/5)
BsaJI 573 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 245 TCCGGA [AT]^CCGG[AT]
BseAI 245 TCCGGA T^CCGGA
BsmI 469 GAATGC GAATGC(1/-1)
Bsp1407I 74 TGTACA T^GTACA
BspEI 245 TCCGGA T^CCGGA
BspHI 140 TCATGA T^CATGA
BsrGI 74 TGTACA T^GTACA
BssHII 578 GCGCGC G^CGCGC
Cfr13I 390 GGACC G^G[ACGT]CC
DdeI 354 CTCAG C^T[ACGT]AG
DraII 389 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 505 CTCTTC CTCTTC(1/4)
EcoNI 230 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 389 GGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 190 AGCGCC [GA]GCGC^[CT]
Hin1I 360 GACGCC G[GA]^CG[CT]C
HinfI 242 GACTC G^A[ACGT]TC
HphI 342 GGTGA GGTGA(8/7)
Ksp632I 505 CTCTTC CTCTTC(1/4)
MboII 506 TCTTC GAAGA(8/7)
MluI 405 ACGCGT A^CGCGT
MroI 245 TCCGGA T^CCGGA
MslI 369 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 594 CCGCTG C[AC]G^C[GT]G
NciI 15 CCGGG CC^[GC]GG
PleI 242 GACTC GAGTC(4/5)
PpuMI 389 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 140 TCATGA T^CATGA
SapI 504 GCTCTTC GCTCTTC(1/4)
Sau96I 390 GGACC G^G[ACGT]CC
SexAI 434 ACCTGGT A^CC[AT]GGT
SmlI 520 CTCAAG C^T[CT][GA]AG
SspBI 74 TGTACA T^GTACA
TaqI 173 TCGA T^CGA
TthHB8I 173 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 405 ACGCGT A^C[GA][CT]GT
425 ACGTGT
AluI 295 AGCT AG^CT
515 AGCT
Alw44I 154 GTGCAC G^TGCAC
553 GTGCAC
ApaLI 154 GTGCAC G^TGCAC
553 GTGCAC
AspHI 154 GTGCAC G[AT]GC[AT]^C
553 GTGCAC
BbrPI 151 CACGTG CAC^GTG
550 CACGTG
BmyI 154 GTGCAC G[AGT]GC[ACT]^C
553 GTGCAC
BsiHKAI 154 GTGCAC G[AT]GC[AT]^C
553 GTGCAC
BsmBI 334 GAGACG CGTCTC(1/5)
358 GAGACG
Bsp1286I 154 GTGCAC G[AGT]GC[ACT]^C
553 GTGCAC
BsrI 488 ACTGG ACTGG(1/-1)
66 CCAGT
BstAPI 492 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
590 GCAACCGCTGC
BstYI 167 AGATCC [GA]^GATC[CT]
326 AGATCC
HapII 15 CCGG C^CGG
246 CCGG
HpaII 15 CCGG C^CGG
246 CCGG
MflI 167 AGATCC [GA]^GATC[CT]
326 AGATCC
MspI 15 CCGG C^CGG
246 CCGG
NlaIII 141 CATG CATG^
606 CATG
NlaIV 349 GGAACC GG[ACGT]^[ACGT]CC
389 GGGACC
PmaCI 151 CACGTG CAC^GTG
550 CACGTG
PmlI 151 CACGTG CAC^GTG
550 CACGTG
PstI 234 CTGCAG CTGCA^G
394 CTGCAG
SfcI 234 CTGCAG C^T[GA][CT]AG
394 CTGCAG
XcmI 8 CCACCTACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
365 CCATCATCATCGTGG
XhoII 167 AGATCC [GA]^GATC[CT]
326 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AfaI AlwI BbvI
BpmI BsaAI BsiYI BslI BsmAI
BsmFI BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I ItaI MaeII
MboI MnlI MvaI MvnI MwoI
NdeII RsaI Sau3AI ScrFI TseI