Restriction map for
pFN21AE3447
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BbeI BbrPI BbvCI BcgI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BssHII
BssSI ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
HgaI Hin1I HindIII HpaI KasI
KspI MluI MroI NarI NdeI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 1511 GGTACC G^GTACC
AccII 3316 CGCG CG^CG
AclI 544 AACGTT AA^CGTT
AocI 3221 CCTCAGG CC^T[ACGT]AGG
ApaI 3694 GGGCCC GGGCC^C
Asp718I 1511 GGTACC G^GTACC
AviII 2404 TGCGCA TGC^GCA
AvrII 798 CCTAGG C^CTAGG
BbsI 3771 GTCTTC GAAGAC(2/6)
BciVI 3799 GGATAC GTATCC(6/5)
BclI 1407 TGATCA T^GATCA
BglI 3715 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1832 AGATCT A^GATCT
BlnI 798 CCTAGG C^CTAGG
BpuAI 3771 GTCTTC GAAGAC(2/6)
BsaAI 3549 TACGTA [CT]AC^GT[GA]
BsaBI 2875 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3226 GGTCTC GGTCTC(1/5)
Bsp1407I 2323 TGTACA T^GTACA
BsrGI 2323 TGTACA T^GTACA
Bst1107I 3546 GTATAC GTA^TAC
BstBI 2739 TTCGAA TT^CGAA
BstEII 1310 GGTTACC G^GT[ACGT]ACC
BstPI 1310 GGTTACC G^GT[ACGT]ACC
BstUI 3316 CGCG CG^CG
BstXI 2601 CCACTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3546 GTATAC GTA^TAC
Bsu36I 3221 CCTCAGG CC^T[ACGT]AGG
DraI 42 TTTAAA TTT^AAA
DsaI 2561 CCATGG C^C[GA][CT]GG
Eco81I 3221 CCTCAGG CC^T[ACGT]AGG
EcoNI 129 CCTACTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1310 GGTTACC G^GT[ACGT]ACC
FbaI 1407 TGATCA T^GATCA
FseI 2413 GGCCGGCC GGCCGG^CC
FspI 2404 TGCGCA TGC^GCA
HaeII 2421 GGCGCT [GA]GCGC^[CT]
HincII 601 GTTGAC GT[CT]^[GA]AC
HindII 601 GTTGAC GT[CT]^[GA]AC
KpnI 1511 GGTACC GGTAC^C
MamI 2875 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3316 CGCG CG^CG
NcoI 2561 CCATGG C^CATGG
NheI 2580 GCTAGC G^CTAGC
NspV 2739 TTCGAA TT^CGAA
PaeR7I 133 CTCGAG C^TCGAG
PflMI 2080 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3677 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 544 AACGTT AA^CGTT
PvuI 171 CGATCG CGAT^CG
ScaI 162 AGTACT AGT^ACT
SfuI 2739 TTCGAA TT^CGAA
SnaBI 3549 TACGTA TAC^GTA
SphI 3633 GCATGC GCATG^C
SspBI 2323 TGTACA T^GTACA
SspI 335 AATATT AAT^ATT
StuI 3218 AGGCCT AGG^CCT
Van91I 2080 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2784 TCTAGA T^CTAGA
XhoI 133 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1629 GTCTAC GT^[AC][GT]AC
3546 GTATAC
AflII 1159 CTTAAG C^TTAAG
1528 CTTAAG
Alw44I 1957 GTGCAC G^TGCAC
3670 GTGCAC
ApaLI 1957 GTGCAC G^TGCAC
3670 GTGCAC
Asp700I 1297 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1450 GAATATCTTC
BalI 1109 TGGCCA TGG^CCA
2669 TGGCCA
BamHI 17 GGATCC G^GATCC
3109 GGATCC
BcnI 804 CCGGG CC^[GC]GG
3510 CCCGG
BfrI 1159 CTTAAG C^TTAAG
1528 CTTAAG
BsmI 2595 GAATGC GAATGC(1/-1)
2217 GCATTC
BsrDI 3597 CATTGC GCAATG(2/0)
2545 GCAATG
CfoI 2405 GCGC GCG^C
2422 GCGC
EagI 2412 CGGCCG C^GGCCG
2416 CGGCCG
EclXI 2412 CGGCCG C^GGCCG
2416 CGGCCG
Eco52I 2412 CGGCCG C^GGCCG
2416 CGGCCG
HhaI 2405 GCGC GCG^C
2422 GCGC
HinP1I 2405 GCGC G^CGC
2422 GCGC
MfeI 1712 CAATTG C^AATTG
2255 CAATTG
MluNI 1109 TGGCCA TGG^CCA
2669 TGGCCA
MscI 1109 TGGCCA TGG^CCA
2669 TGGCCA
MunI 1712 CAATTG C^AATTG
2255 CAATTG
NciI 804 CCGGG CC^[GC]GG
3510 CCCGG
SacI 1289 GAGCTC GAGCT^C
3308 GAGCTC
XcmI 848 CCAAGGAAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3756 CCAATCCTACATTGG
XmnI 1297 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1450 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII