Restriction map for
pFN21AB0120
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BclI BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BspHI BsrBI BsrDI BssHII BssSI
Bsu36I CelII CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI Hin1I HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SpeI Sse8387I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 3923 TCCGGA T^CCGGA
AclI 2992 AACGTT AA^CGTT
AflII 2349 CTTAAG C^TTAAG
AseI 3049 ATTAAT AT^TAAT
AsnI 3049 ATTAAT AT^TAAT
AviII 137 TGCGCA TGC^GCA
BbvCI 4482 GCTGAGG CCTCAGC(-5/-2)
BfrI 2349 CTTAAG C^TTAAG
BglI 4049 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1004 AGATCT A^GATCT
BsaWI 3923 TCCGGA [AT]^CCGG[AT]
BseAI 3923 TCCGGA T^CCGGA
BsmBI 1615 CGTCTC CGTCTC(1/5)
Bsp1407I 695 TGTACA T^GTACA
BspEI 3923 TCCGGA T^CCGGA
BsrFI 3661 ACCGGC [GA]^CCGG[CT]
BsrGI 695 TGTACA T^GTACA
Bst1107I 3081 GTATAC GTA^TAC
BstAPI 1018 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4510 TTCGAA TT^CGAA
BstEII 775 GGTCACC G^GT[ACGT]ACC
BstPI 775 GGTCACC G^GT[ACGT]ACC
BstXI 3882 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3081 GTATAC GTA^TAC
Cfr10I 3661 ACCGGC [GA]^CCGG[CT]
DraI 183 TTTAAA TTT^AAA
DrdI 2857 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 775 GGTCACC G^GT[ACGT]ACC
EcoRI 1050 GAATTC G^AATTC
EcoT22I 1273 ATGCAT ATGCA^T
FspI 137 TGCGCA TGC^GCA
HaeII 132 GGCGCT [GA]GCGC^[CT]
HgaI 1614 GCGTC GACGC(5/10)
MfeI 2564 CAATTG C^AATTG
MroI 3923 TCCGGA T^CCGGA
MunI 2564 CAATTG C^AATTG
NheI 1320 GCTAGC G^CTAGC
NsiI 1273 ATGCAT ATGCA^T
NspV 4510 TTCGAA TT^CGAA
PshBI 3049 ATTAAT AT^TAAT
Psp1406I 2992 AACGTT AA^CGTT
SacI 3398 GAGCTC GAGCT^C
SfuI 4510 TTCGAA TT^CGAA
SphI 3848 GCATGC GCATG^C
SspBI 695 TGTACA T^GTACA
SspI 2740 AATATT AAT^ATT
StuI 679 AGGCCT AGG^CCT
SwaI 182 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 850 GTAGAC GT^[AC][GT]AC
3081 GTATAC
AccII 4 CGCG CG^CG
3618 CGCG
AflIII 2794 ACATGT A^C[GA][CT]GT
3034 ACATGT
ApaI 711 GGGCCC GGGCC^C
4090 GGGCCC
Asp700I 432 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1756 GAAATACTTC
AvaI 102 CTCGGG C^[CT]CG[GA]G
683 CTCGGG
BalI 1985 TGGCCA TGG^CCA
2114 TGGCCA
BbsI 213 GAAGAC GAAGAC(2/6)
3574 GAAGAC
BcgI 4435 CGATTAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
839 GCAACAATATCG
BciVI 2202 GGATAC GTATCC(6/5)
4321 GGATAC
BpmI 950 CTGGAG CTGGAG(16/14)
2666 CTGGAG
BpuAI 213 GAAGAC GAAGAC(2/6)
3574 GAAGAC
BsoBI 102 CTCGGG C^[CT]CG[GA]G
683 CTCGGG
BspDI 1475 ATCGAT AT^CGAT
3223 ATCGAT
BspLU11I 2794 ACATGT A^CATGT
3034 ACATGT
BspMI 4063 ACCTGC ACCTGC(4/8)
4150 ACCTGC
BstUI 4 CGCG CG^CG
3618 CGCG
ClaI 1475 ATCGAT AT^CGAT
3223 ATCGAT
DraIII 699 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3721 CACATTGTG
EaeI 1985 TGGCCA [CT]^GGCC[GA]
2114 TGGCCA
EcoNI 57 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3591 CCTGTATAAGG
FauI 161 CCCGC CCCGC(4/6)
1379 CCCGC
MluNI 1985 TGGCCA TGG^CCA
2114 TGGCCA
MscI 1985 TGGCCA TGG^CCA
2114 TGGCCA
MslI 2497 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4201 CATGGAGGTG
MvnI 4 CGCG CG^CG
3618 CGCG
PflMI 2106 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3720 CCACATTGTGG
SmlI 1312 CTCAAG C^T[CT][GA]AG
2349 CTTAAG
SnaBI 1679 TACGTA TAC^GTA
3202 TACGTA
Van91I 2106 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3720 CCACATTGTGG
XcmI 2102 CCATCCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3683 CCATTTACAGTGTGG
XmnI 432 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1756 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII