Restriction map for
pFN21AE0987
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AvrII BbeI BbrPI
BbsI BcgI BciVI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 590 ACGTGT A^C[GA][CT]GT
AhdI 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1534 CCTGAGG CC^T[ACGT]AGG
Asp700I 306 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 478 GGATCC G^GATCC
BbvCI 1186 GCTGAGG CCTCAGC(-5/-2)
BclI 968 TGATCA T^GATCA
BfaI 193 CTAG C^TAG
BsaAI 508 TACGTG [CT]AC^GT[GA]
BsmBI 814 GAGACG CGTCTC(1/5)
BsrBI 1782 CCGCTC CCGCTC(-3/-3)
BsrFI 1222 GCCGGC [GA]^CCGG[CT]
BssSI 1066 CACGAG CACGAG(-5/-1)
BstXI 1788 CCACCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1222 GCCGGC [GA]^CCGG[CT]
DraIII 1390 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 589 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1534 CCTGAGG CC^T[ACGT]AGG
FbaI 968 TGATCA T^GATCA
MaeI 193 CTAG C^TAG
NaeI 1222 GCCGGC GCC^GGC
NcoI 1655 CCATGG C^CATGG
NgoMIV 1222 GCCGGC G^CCGGC
PshAI 1291 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1681 GAGCTC GAGCT^C
SapI 1683 GCTCTTC GCTCTTC(1/4)
SbfI 351 CCTGCAGG CCTGCA^GG
SmaI 1805 CCCGGG CCC^GGG
Sse8387I 351 CCTGCAGG CCTGCA^GG
StuI 189 AGGCCT AGG^CCT
XmaCI 1805 CCCGGG C^CCGGG
XmaI 1805 CCCGGG C^CCGGG
XmnI 306 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 315 CGCG CG^CG
533 CGCG
AcyI 133 GACGCC G[GA]^CG[CT]C
241 GACGCC
AfaI 341 GTAC GT^AC
660 GTAC
AviII 1055 TGCGCA TGC^GCA
1097 TGCGCA
BanI 1254 GGCACC G^G[CT][GA]CC
1841 GGCACC
BglI 724 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1442 GCCTGGCAGGC
BsaHI 133 GACGCC G[GA]^CG[CT]C
241 GACGCC
BseRI 1189 GAGGAG GAGGAG(10/8)
1246 GAGGAG
BstAPI 177 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1047 GCAGCAGCTGC
BstUI 315 CGCG CG^CG
533 CGCG
DrdI 984 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1354 GACATGATGGTC
DsaI 1655 CCATGG C^C[GA][CT]GG
1756 CCACGG
EarI 1684 CTCTTC CTCTTC(1/4)
202 GAAGAG
EcoT14I 77 CCAAGG C^C[AT][AT]GG
1655 CCATGG
FspI 1055 TGCGCA TGC^GCA
1097 TGCGCA
HaeII 836 AGCGCC [GA]GCGC^[CT]
927 GGCGCT
Hin1I 133 GACGCC G[GA]^CG[CT]C
241 GACGCC
Ksp632I 1684 CTCTTC CTCTTC(1/4)
202 GAAGAG
MvnI 315 CGCG CG^CG
533 CGCG
PflMI 77 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
773 CCAACCCCTGG
PpuMI 58 GGGACCT [GA]G^G[AT]CC[CT]
1165 GGGACCC
RsaI 341 GTAC GT^AC
660 GTAC
SphI 182 GCATGC GCATG^C
977 GCATGC
StyI 77 CCAAGG C^C[AT][AT]GG
1655 CCATGG
Van91I 77 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
773 CCAACCCCTGG
XcmI 1139 CCACGCCCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1274 CCATCGACCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI TaqI TseI Tsp45I
TspRI TthHB8I XhoII