Restriction map for
pFN21AE4114
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI MaeI MamI MfeI MflI
MluI MseI MunI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1206 GGTACC G^GTACC
AccI 382 GTCTAC GT^[AC][GT]AC
AcyI 1082 GGCGCC G[GA]^CG[CT]C
Alw44I 268 GTGCAC G^TGCAC
AlwI 522 GATCC GGATC(4/5)
AocI 231 CCTGAGG CC^T[ACGT]AGG
Aor51HI 989 AGCGCT AGC^GCT
ApaLI 268 GTGCAC G^TGCAC
Asp718I 1206 GGTACC G^GTACC
BbeI 1082 GGCGCC GGCGC^C
BbvCI 1434 CCTCAGC CCTCAGC(-5/-2)
BcgI 1185 CGAAGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 285 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 285 GCTCAGC GC^T[ACGT]AGC
BsaHI 1082 GGCGCC G[GA]^CG[CT]C
BsmI 784 GCATTC GAATGC(1/-1)
BsrDI 698 GCAATG GCAATG(2/0)
BssSI 1375 CACGAG CACGAG(-5/-1)
BstAPI 107 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 50 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 231 CCTGAGG CC^T[ACGT]AGG
CelII 285 GCTCAGC GC^T[ACGT]AGC
EarI 853 CTCTTC CTCTTC(1/4)
Eco47III 989 AGCGCT AGC^GCT
Eco81I 231 CCTGAGG CC^T[ACGT]AGG
FseI 1221 GGCCGGCC GGCCGG^CC
HgaI 1010 GCGTC GACGC(5/10)
Hin1I 1082 GGCGCC G[GA]^CG[CT]C
KasI 1082 GGCGCC G^GCGCC
KpnI 1206 GGTACC GGTAC^C
Ksp632I 853 CTCTTC CTCTTC(1/4)
KspI 1023 CCGCGG CCGC^GG
MaeII 203 ACGT A^CGT
NarI 1082 GGCGCC GG^CGCC
NspI 626 ACATGC [GA]CATG^[CT]
PleI 499 GAGTC GAGTC(4/5)
PstI 85 CTGCAG CTGCA^G
SacII 1023 CCGCGG CCGC^GG
SapI 852 GCTCTTC GCTCTTC(1/4)
SexAI 551 ACCTGGT A^CC[AT]GGT
SfoI 1082 GGCGCC GGC^GCC
SmlI 886 CTCAAG C^T[CT][GA]AG
StuI 810 AGGCCT AGG^CCT
TfiI 488 GAATC G^A[AT]TC
XcmI 200 CCAACGTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccII 529 CGCG CG^CG
1024 CGCG
AccIII 722 TCCGGA T^CCGGA
857 TCCGGA
AfaI 1182 GTAC GT^AC
1207 GTAC
AlwNI 538 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1002 CAGCACCTG
BanI 1082 GGCGCC G^G[CT][GA]CC
1206 GGTACC
BbsI 64 GTCTTC GAAGAC(2/6)
508 GTCTTC
BglI 1112 GCCGTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1416 GCCCAGCGGGC
BpuAI 64 GTCTTC GAAGAC(2/6)
508 GTCTTC
BseAI 722 TCCGGA T^CCGGA
857 TCCGGA
BseRI 400 GAGGAG GAGGAG(10/8)
562 GAGGAG
BsmAI 205 GTCTC GTCTC(1/5)
373 GTCTC
BsmBI 204 CGTCTC CGTCTC(1/5)
372 CGTCTC
BspEI 722 TCCGGA T^CCGGA
857 TCCGGA
BstUI 529 CGCG CG^CG
1024 CGCG
DraII 245 GGGACCC [GA]G^G[ACGT]CC[CT]
1132 AGGTCCC
DraIII 550 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1144 CACGGCGTG
EagI 1038 CGGCCG C^GGCCG
1309 CGGCCG
EclXI 1038 CGGCCG C^GGCCG
1309 CGGCCG
Eco52I 1038 CGGCCG C^GGCCG
1309 CGGCCG
EcoNI 813 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1164 CCTGAAGGAGG
EcoO109I 245 GGGACCC [GA]G^G[ACGT]CC[CT]
1132 AGGTCCC
HaeII 989 AGCGCT [GA]GCGC^[CT]
1082 GGCGCC
HinfI 488 GAATC G^A[ACGT]TC
499 GAGTC
MaeIII 364 GTCAC ^GT[ACGT]AC
596 GTCAC
MroI 722 TCCGGA T^CCGGA
857 TCCGGA
MvnI 529 CGCG CG^CG
1024 CGCG
NaeI 167 GCCGGC GCC^GGC
1222 GCCGGC
NcoI 737 CCATGG C^CATGG
1400 CCATGG
NgoMIV 167 GCCGGC G^CCGGC
1222 GCCGGC
NotI 1037 GCGGCCGC GC^GGCCGC
1308 GCGGCCGC
PflMI 549 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCACTCCATGG
PpuMI 245 GGGACCC [GA]G^G[AT]CC[CT]
1132 AGGTCCC
PvuII 513 CAGCTG CAG^CTG
631 CAGCTG
RsaI 1182 GTAC GT^AC
1207 GTAC
SmaI 653 CCCGGG CCC^GGG
1202 CCCGGG
Tsp45I 364 GTCAC ^GT[GC]AC
596 GTCAC
Van91I 549 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
732 CCACTCCATGG
XmaCI 653 CCCGGG C^CCGGG
1202 CCCGGG
XmaI 653 CCCGGG C^CCGGG
1202 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I