Restriction map for
pFN21AE3534
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AcsI AflII
AflIII AgeI AocI ApoI AseI
AsnI Asp700I Asp718I AvrII BalI
BbrPI BbsI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI BstBI BstEII BstPI Bsu36I
CelII ClaI DraI DrdI EarI
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI SacI SacII SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AclI 976 AACGTT AA^CGTT
AhdI 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 447 GTGCAC G^TGCAC
AlwNI 166 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 153 AGCGCT AGC^GCT
ApaLI 447 GTGCAC G^TGCAC
AscI 357 GGCGCGCC GG^CGCGCC
AspEI 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 447 GTGCAC G[AT]GC[AT]^C
AviII 752 TGCGCA TGC^GCA
BamHI 1410 GGATCC G^GATCC
BbvCI 156 GCTGAGG CCTCAGC(-5/-2)
BciVI 139 GTATCC GTATCC(6/5)
BglI 27 GCCGTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1263 CACGTA [CT]AC^GT[GA]
BsiHKAI 447 GTGCAC G[AT]GC[AT]^C
BsmBI 889 CGTCTC CGTCTC(1/5)
BsrDI 1327 GCAATG GCAATG(2/0)
Bst1107I 1075 GTATAC GTA^TAC
BstYI 1410 GGATCC [GA]^GATC[CT]
BstZ17I 1075 GTATAC GTA^TAC
CpoI 416 CGGTCCG CG^G[AT]CCG
DraIII 1559 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1781 CCATGG C^C[GA][CT]GG
Eam1105I 1852 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 153 AGCGCT AGC^GCT
FspI 752 TGCGCA TGC^GCA
MflI 1410 GGATCC [GA]^GATC[CT]
MslI 1899 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1781 CCATGG C^CATGG
NdeI 1645 CATATG CA^TATG
PflMI 777 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1826 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 976 AACGTT AA^CGTT
RsrII 416 CGGTCCG CG^G[AT]CCG
SalI 207 GTCGAC G^TCGAC
SgrAI 52 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 99 CTTGAG C^T[CT][GA]AG
Tsp509I 1581 AATT ^AATT
Van91I 777 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1301 CCATACTGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1410 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 207 GTCGAC GT^[AC][GT]AC
1075 GTATAC
AfaI 867 GTAC GT^AC
1266 GTAC
AlwI 1411 GATCC GGATC(4/5)
1410 GGATC
AspI 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
495 GACGGCGTC
BbeI 50 GGCGCC GGCGC^C
659 GGCGCC
BfaI 176 CTAG C^TAG
1912 CTAG
BsaI 430 GAGACC GGTCTC(1/5)
817 GAGACC
BseRI 892 CTCCTC GAGGAG(10/8)
760 GAGGAG
BsmI 1222 GAATGC GAATGC(1/-1)
1395 GCATTC
BspMI 1829 ACCTGC ACCTGC(4/8)
510 GCAGGT
BsrBI 791 CCGCTC CCGCTC(-3/-3)
602 GAGCGG
BstAPI 165 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1161 GCAGACCGTGC
BstXI 1579 CCAATTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1745 CCAAGTGCTTGG
DpnII 646 GATC ^GATC
1411 GATC
EcoNI 393 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
864 CCTGTACCAGG
EcoT14I 1781 CCATGG C^C[AT][AT]GG
1862 CCTTGG
HincII 207 GTCGAC GT[CT]^[GA]AC
1623 GTTGAC
HindII 207 GTCGAC GT[CT]^[GA]AC
1623 GTTGAC
KasI 50 GGCGCC G^GCGCC
659 GGCGCC
MaeI 176 CTAG C^TAG
1912 CTAG
MaeIII 1286 GTGAC ^GT[ACGT]AC
1838 GTGAC
MboI 646 GATC ^GATC
1411 GATC
NarI 50 GGCGCC GG^CGCC
659 GGCGCC
NdeII 646 GATC ^GATC
1411 GATC
PflFI 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
495 GACGGCGTC
RsaI 867 GTAC GT^AC
1266 GTAC
Sau3AI 646 GATC ^GATC
1411 GATC
SfoI 50 GGCGCC GGC^GCC
659 GGCGCC
StuI 320 AGGCCT AGG^CCT
1034 AGGCCT
StyI 1781 CCATGG C^C[AT][AT]GG
1862 CCTTGG
Tsp45I 1286 GTGAC ^GT[GC]AC
1838 GTGAC
Tth111I 348 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
495 GACGGCGTC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PleI
PstI PvuII Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI TspRI
TthHB8I