Restriction map for
pFN21AE3538
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BbvCI BcgI BclI BfrI BlnI
BpuAI BseAI BsiWI Bsp1407I BspDI
BspEI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflIII 2081 ACATGT A^C[GA][CT]GT
Alw44I 1593 GTGCAC G^TGCAC
ApaLI 1593 GTGCAC G^TGCAC
Asp700I 2400 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1435 GGCGCC GGCGC^C
BbrPI 1705 CACGTG CAC^GTG
BciVI 1026 GGATAC GTATCC(6/5)
BfaI 2137 CTAG C^TAG
BglII 1856 AGATCT A^GATCT
BsiEI 1794 CGACCG CG[GA][CT]^CG
BsmBI 2347 CGTCTC CGTCTC(1/5)
BsmI 703 GCATTC GAATGC(1/-1)
BspHI 1688 TCATGA T^CATGA
BspLU11I 2081 ACATGT A^CATGT
BsrBI 181 GAGCGG CCGCTC(-3/-3)
DsaI 71 CCATGG C^C[GA][CT]GG
KasI 1435 GGCGCC G^GCGCC
MaeI 2137 CTAG C^TAG
NarI 1435 GGCGCC GG^CGCC
NcoI 71 CCATGG C^CATGG
PaeR7I 1994 CTCGAG C^TCGAG
PmaCI 1705 CACGTG CAC^GTG
PmlI 1705 CACGTG CAC^GTG
PpuMI 275 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1688 TCATGA T^CATGA
SexAI 843 ACCAGGT A^CC[AT]GGT
SfoI 1435 GGCGCC GGC^GCC
XhoI 1994 CTCGAG C^TCGAG
XmnI 2400 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 2163 GAATTT [GA]^AATT[CT]
2440 AAATTT
AcyI 1435 GGCGCC G[GA]^CG[CT]C
1729 GACGCC
AocI 469 CCTGAGG CC^T[ACGT]AGG
771 CCTCAGG
ApoI 2163 GAATTT [GA]^AATT[CT]
2440 AAATTT
BglI 205 GCCTCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
942 GCCTCCAGGGC
BlpI 522 GCTGAGC GC^T[ACGT]AGC
1698 GCTCAGC
Bpu1102I 522 GCTGAGC GC^T[ACGT]AGC
1698 GCTCAGC
BsaAI 1705 CACGTG [CT]AC^GT[GA]
1771 TACGTG
BsaBI 1002 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1680 GATCAACATC
BsaHI 1435 GGCGCC G[GA]^CG[CT]C
1729 GACGCC
BsaWI 1398 TCCGGT [AT]^CCGG[AT]
1796 ACCGGA
Bsu36I 469 CCTGAGG CC^T[ACGT]AGG
771 CCTCAGG
CelII 522 GCTGAGC GC^T[ACGT]AGC
1698 GCTCAGC
DraIII 842 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2283 CACACAGTG
Eco81I 469 CCTGAGG CC^T[ACGT]AGG
771 CCTCAGG
EcoNI 420 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1332 CCTGCAGCAGG
Hin1I 1435 GGCGCC G[GA]^CG[CT]C
1729 GACGCC
HindIII 1284 AAGCTT A^AGCTT
2327 AAGCTT
MamI 1002 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1680 GATCAACATC
PflMI 651 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
916 CCAGGGCCTGG
PvuII 1097 CAGCTG CAG^CTG
1749 CAGCTG
SacI 1195 GAGCTC GAGCT^C
1786 GAGCTC
SmlI 1909 CTCAAG C^T[CT][GA]AG
1994 CTCGAG
Van91I 651 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
916 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI