Restriction map for
pFN21AE3553
##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AspI BbrPI BsiWI
Bsp1407I BspDI BsrGI BssHII BstBI
BstEII BstPI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI HpaI
MluI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 5732 GGCGCC G[GA]^CG[CT]C
ApaI 5837 GGGCCC GGGCC^C
AvaI 5129 CCCGAG C^[CT]CG[GA]G
AviII 5678 TGCGCA TGC^GCA
BamHI 510 GGATCC G^GATCC
BbeI 5732 GGCGCC GGCGC^C
BcgI 2384 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2543 TGATCA T^GATCA
BlpI 2702 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2702 GCTCAGC GC^T[ACGT]AGC
BsaBI 3622 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5732 GGCGCC G[GA]^CG[CT]C
BsmBI 5228 GAGACG CGTCTC(1/5)
BsoBI 5129 CCCGAG C^[CT]CG[GA]G
Bst1107I 970 GTATAC GTA^TAC
BstZ17I 970 GTATAC GTA^TAC
CelII 2702 GCTCAGC GC^T[ACGT]AGC
DraIII 5243 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 639 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4294 GAATTC G^AATTC
FbaI 2543 TGATCA T^GATCA
FspI 5678 TGCGCA TGC^GCA
Hin1I 5732 GGCGCC G[GA]^CG[CT]C
KasI 5732 GGCGCC G^GCGCC
KspI 5860 CCGCGG CCGC^GG
MamI 3622 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4415 CAATTG C^AATTG
MunI 4415 CAATTG C^AATTG
NarI 5732 GGCGCC GG^CGCC
NdeI 65 CATATG CA^TATG
SacII 5860 CCGCGG CCGC^GG
SbfI 2409 CCTGCAGG CCTGCA^GG
SexAI 479 ACCTGGT A^CC[AT]GGT
SfoI 5732 GGCGCC GGC^GCC
Sse8387I 2409 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 998 GGTACC G^GTACC
4867 GGTACC
AccIII 513 TCCGGA T^CCGGA
4200 TCCGGA
AflII 3987 CTTAAG C^TTAAG
4590 CTTAAG
AflIII 974 ACATGT A^C[GA][CT]GT
5333 ACATGT
AseI 3450 ATTAAT AT^TAAT
4492 ATTAAT
AsnI 3450 ATTAAT AT^TAAT
4492 ATTAAT
Asp700I 4059 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4290 GAATGAATTC
Asp718I 998 GGTACC G^GTACC
4867 GGTACC
AvrII 487 CCTAGG C^CTAGG
3162 CCTAGG
BbvCI 2689 CCTCAGC CCTCAGC(-5/-2)
2658 GCTGAGG
BfrI 3987 CTTAAG C^TTAAG
4590 CTTAAG
BglI 1675 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5430 GCCTGCCGGGC
BlnI 487 CCTAGG C^CTAGG
3162 CCTAGG
BsaAI 2053 TACGTG [CT]AC^GT[GA]
2555 CACGTA
BseAI 513 TCCGGA T^CCGGA
4200 TCCGGA
BsiEI 408 CGACCG CG[GA][CT]^CG
843 CGGTCG
BspEI 513 TCCGGA T^CCGGA
4200 TCCGGA
BspLU11I 974 ACATGT A^CATGT
5333 ACATGT
BssSI 19 CACGAG CACGAG(-5/-1)
4823 CTCGTG
BstAPI 4338 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5202 GCAGAGGATGC
DraI 1969 TTTAAA TTT^AAA
3307 TTTAAA
DsaI 2444 CCGTGG C^C[GA][CT]GG
5860 CCGCGG
HaeII 428 AGCGCC [GA]GCGC^[CT]
5732 GGCGCC
HgaI 4886 GACGC GACGC(5/10)
5711 GCGTC
HincII 4729 GTTGAC GT[CT]^[GA]AC
4912 GTCAAC
HindII 4729 GTTGAC GT[CT]^[GA]AC
4912 GTCAAC
KpnI 998 GGTACC GGTAC^C
4867 GGTACC
MroI 513 TCCGGA T^CCGGA
4200 TCCGGA
PpuMI 2537 AGGACCT [GA]G^G[AT]CC[CT]
4178 AGGTCCT
PshBI 3450 ATTAAT AT^TAAT
4492 ATTAAT
XmnI 4059 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4290 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII