Restriction map for
pFN21AE4255
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BalI BbvCI BcgI BclI BfrI
BglII BlnI BsaBI BsaI BseRI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FbaI FseI FspI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI StuI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1730 GTCGAC GT^[AC][GT]AC
AccIII 972 TCCGGA T^CCGGA
AcyI 16 GGCGCC G[GA]^CG[CT]C
AseI 2116 ATTAAT AT^TAAT
AsnI 2116 ATTAAT AT^TAAT
AspI 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 539 GGATCC G^GATCC
BanI 16 GGCGCC G^G[CT][GA]CC
BbeI 16 GGCGCC GGCGC^C
BbrPI 1106 CACGTG CAC^GTG
BglI 562 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BsaWI 972 TCCGGA [AT]^CCGG[AT]
BseAI 972 TCCGGA T^CCGGA
BsmBI 2595 CGTCTC CGTCTC(1/5)
BsmI 2285 GAATGC GAATGC(1/-1)
BspEI 972 TCCGGA T^CCGGA
BspMI 1830 ACCTGC ACCTGC(4/8)
BssHII 176 GCGCGC G^CGCGC
BstAPI 736 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 67 GCTGAGC GC^T[ACGT]AGC
EaeI 83 CGGCCA [CT]^GGCC[GA]
Hin1I 16 GGCGCC G[GA]^CG[CT]C
HindIII 1430 AAGCTT A^AGCTT
HpaI 1338 GTTAAC GTT^AAC
KasI 16 GGCGCC G^GCGCC
MroI 972 TCCGGA T^CCGGA
NarI 16 GGCGCC GG^CGCC
PaeR7I 1381 CTCGAG C^TCGAG
PflFI 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1106 CACGTG CAC^GTG
PmlI 1106 CACGTG CAC^GTG
PshBI 2116 ATTAAT AT^TAAT
SacI 1400 GAGCTC GAGCT^C
SalI 1730 GTCGAC G^TCGAC
SapI 1402 GCTCTTC GCTCTTC(1/4)
ScaI 1189 AGTACT AGT^ACT
SexAI 1496 ACCTGGT A^CC[AT]GGT
SfoI 16 GGCGCC GGC^GCC
SmlI 1381 CTCGAG C^T[CT][GA]AG
SpeI 1096 ACTAGT A^CTAGT
SphI 2868 GCATGC GCATG^C
SspI 2959 AATATT AAT^ATT
SwaI 2312 ATTTAAAT ATTT^AAAT
Tth111I 2030 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2433 TCTAGA T^CTAGA
XcmI 914 CCACTGGTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1381 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 177 CGCG CG^CG
184 CGCG
AfaI 3 GTAC GT^AC
1190 GTAC
AflIII 1105 ACACGT A^C[GA][CT]GT
1375 ACGTGT
Alw44I 692 GTGCAC G^TGCAC
907 GTGCAC
ApaLI 692 GTGCAC G^TGCAC
907 GTGCAC
BanII 569 GGGCTC G[GA]GC[CT]^C
1400 GAGCTC
BsaAI 1106 CACGTG [CT]AC^GT[GA]
1374 TACGTG
BssSI 135 CTCGTG CACGAG(-5/-1)
235 CTCGTG
BstUI 177 CGCG CG^CG
184 CGCG
BstXI 1295 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2531 CCATACCTGTGG
BstYI 539 GGATCC [GA]^GATC[CT]
2525 GGATCT
DsaI 468 CCATGG C^C[GA][CT]GG
1154 CCATGG
EcoRI 331 GAATTC G^AATTC
2739 GAATTC
EcoT22I 1471 ATGCAT ATGCA^T
1963 ATGCAT
HgaI 167 GACGC GACGC(5/10)
2058 GCGTC
MflI 539 GGATCC [GA]^GATC[CT]
2525 GGATCT
MvnI 177 CGCG CG^CG
184 CGCG
NcoI 468 CCATGG C^CATGG
1154 CCATGG
NdeI 902 CATATG CA^TATG
1064 CATATG
NsiI 1471 ATGCAT ATGCA^T
1963 ATGCAT
PpuMI 128 GGGTCCT [GA]G^G[AT]CC[CT]
526 AGGTCCC
RsaI 3 GTAC GT^AC
1190 GTAC
SmaI 61 CCCGGG CCC^GGG
1114 CCCGGG
TaqI 1382 TCGA T^CGA
1731 TCGA
Tsp45I 950 GTGAC ^GT[GC]AC
1078 GTGAC
TthHB8I 1382 TCGA T^CGA
1731 TCGA
XhoII 539 GGATCC [GA]^GATC[CT]
2525 GGATCT
XmaCI 61 CCCGGG C^CCGGG
1114 CCCGGG
XmaI 61 CCCGGG C^CCGGG
1114 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaI AvaII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI Van91I XmnI