Restriction map for
pFN21AE3334
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccIII AflII AflIII AgeI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI AspI AvrII BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eco81I EcoO65I EcoRV
FbaI FseI HincII HindII HpaI
KspI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1495 GGTACC G^GTACC
AccI 36 GTCTAC GT^[AC][GT]AC
AclI 1787 AACGTT AA^CGTT
AhdI 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2354 AGCGCT AGC^GCT
Asp700I 1815 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1495 GGTACC G^GTACC
AspEI 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2321 TGCGCA TGC^GCA
BamHI 1922 GGATCC G^GATCC
BfaI 424 CTAG C^TAG
BsaBI 243 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 169 GAGACG CGTCTC(1/5)
BsrFI 1349 GCCGGC [GA]^CCGG[CT]
Cfr10I 1349 GCCGGC [GA]^CCGG[CT]
Eam1105I 655 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2354 AGCGCT AGC^GCT
EcoNI 1929 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1262 GAATTC G^AATTC
EcoT22I 1780 ATGCAT ATGCA^T
FspI 2321 TGCGCA TGC^GCA
HindIII 2746 AAGCTT A^AGCTT
KpnI 1495 GGTACC GGTAC^C
MaeI 424 CTAG C^TAG
MamI 243 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2451 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1349 GCCGGC GCC^GGC
NgoMIV 1349 GCCGGC G^CCGGC
NsiI 1780 ATGCAT ATGCA^T
NspI 2603 ACATGC [GA]CATG^[CT]
PflMI 2664 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1241 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1787 AACGTT AA^CGTT
ScaI 2294 AGTACT AGT^ACT
SexAI 1951 ACCAGGT A^CC[AT]GGT
StuI 381 AGGCCT AGG^CCT
Van91I 2664 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1397 CCAATGGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1815 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 1262 GAATTC [GA]^AATT[CT]
1594 AAATTT
ApoI 1262 GAATTC [GA]^AATT[CT]
1594 AAATTT
BbeI 133 GGCGCC GGCGC^C
1685 GGCGCC
BbsI 549 GAAGAC GAAGAC(2/6)
955 GAAGAC
BbvCI 1308 CCTCAGC CCTCAGC(-5/-2)
922 GCTGAGG
BpuAI 549 GAAGAC GAAGAC(2/6)
955 GAAGAC
BsgI 442 CTGCAC GTGCAG(16/14)
1470 CTGCAC
BsiEI 960 CGGCCG CG[GA][CT]^CG
2752 CGGCCG
Bsp1407I 1565 TGTACA T^GTACA
2459 TGTACA
BspHI 323 TCATGA T^CATGA
2186 TCATGA
BspMI 259 ACCTGC ACCTGC(4/8)
1416 ACCTGC
BsrBI 2338 GAGCGG CCGCTC(-3/-3)
2560 GAGCGG
BsrGI 1565 TGTACA T^GTACA
2459 TGTACA
DsaI 350 CCGTGG C^C[GA][CT]GG
989 CCGTGG
EagI 960 CGGCCG C^GGCCG
2752 CGGCCG
EclXI 960 CGGCCG C^GGCCG
2752 CGGCCG
Eco52I 960 CGGCCG C^GGCCG
2752 CGGCCG
EcoT14I 929 CCAAGG C^C[AT][AT]GG
1376 CCTTGG
KasI 133 GGCGCC G^GCGCC
1685 GGCGCC
NarI 133 GGCGCC GG^CGCC
1685 GGCGCC
PaeR7I 499 CTCGAG C^TCGAG
1072 CTCGAG
RcaI 323 TCATGA T^CATGA
2186 TCATGA
SacI 715 GAGCTC GAGCT^C
2200 GAGCTC
SfoI 133 GGCGCC GGC^GCC
1685 GGCGCC
SspBI 1565 TGTACA T^GTACA
2459 TGTACA
StyI 929 CCAAGG C^C[AT][AT]GG
1376 CCTTGG
TfiI 543 GAATC G^A[AT]TC
568 GATTC
XhoI 499 CTCGAG C^TCGAG
1072 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BglII BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII