################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI BalI BbeI BbrPI BbvCI BcgI BclI BglI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FbaI FseI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Asp700I 2394 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3902 TGCGCA TGC^GCA AvrII 1762 CCTAGG C^CTAGG BamHI 819 GGATCC G^GATCC BciVI 3279 GGATAC GTATCC(6/5) BlnI 1762 CCTAGG C^CTAGG BlpI 771 GCTCAGC GC^T[ACGT]AGC Bpu1102I 771 GCTCAGC GC^T[ACGT]AGC BsaAI 2046 CACGTA [CT]AC^GT[GA] BsaI 3232 GGTCTC GGTCTC(1/5) BsmI 1194 GCATTC GAATGC(1/-1) BspMI 3640 GCAGGT ACCTGC(4/8) Bst1107I 3223 GTATAC GTA^TAC BstZ17I 3223 GTATAC GTA^TAC CelII 771 GCTCAGC GC^T[ACGT]AGC CfoI 3903 GCGC GCG^C DraIII 2111 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 825 GACTCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 903 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 3902 TGCGCA TGC^GCA HhaI 3903 GCGC GCG^C HinP1I 3903 GCGC G^CGC HindIII 1323 AAGCTT A^AGCTT MfeI 1756 CAATTG C^AATTG MspA1I 2562 CAGCTG C[AC]G^C[GT]G MunI 1756 CAATTG C^AATTG NruI 3552 TCGCGA TCG^CGA PvuII 2562 CAGCTG CAG^CTG SpeI 3238 ACTAGT A^CTAGT StuI 1747 AGGCCT AGG^CCT XbaI 2670 TCTAGA T^CTAGA XcmI 3806 CCATGGACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2394 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 3821 CCGC CCGC(-3/-1) 414 GCGG BanI 1443 GGTGCC G^G[CT][GA]CC 3652 GGCACC BcnI 793 CCGGG CC^[GC]GG 1722 CCCGG BsaWI 1811 ACCGGA [AT]^CCGG[AT] 3314 ACCGGA BspHI 54 TCATGA T^CATGA 2784 TCATGA BspLU11I 863 ACATGT A^CATGT 3357 ACATGT BstXI 2354 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3623 CCAGGCGTTTGG Cac8I 2647 GCCAGC GC[ACGT]^[ACGT]GC 3389 GCTTGC DraI 3156 TTTAAA TTT^AAA 3645 TTTAAA DraII 1671 AGGTCCC [GA]G^G[ACGT]CC[CT] 1999 GGGACCC DsaI 1053 CCATGG C^C[GA][CT]GG 3806 CCATGG EaeI 603 TGGCCG [CT]^GGCC[GA] 3818 TGGCCG EcoO109I 1671 AGGTCCC [GA]G^G[ACGT]CC[CT] 1999 GGGACCC EcoRV 3556 GATATC GAT^ATC 3745 GATATC EcoT22I 1690 ATGCAT ATGCA^T 3679 ATGCAT HincII 1552 GTCAAC GT[CT]^[GA]AC 3361 GTCAAC HindII 1552 GTCAAC GT[CT]^[GA]AC 3361 GTCAAC MaeII 2047 ACGT A^CGT 2085 ACGT NciI 793 CCGGG CC^[GC]GG 1722 CCCGG NcoI 1053 CCATGG C^CATGG 3806 CCATGG NdeI 589 CATATG CA^TATG 2164 CATATG NsiI 1690 ATGCAT ATGCA^T 3679 ATGCAT PflMI 1745 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2964 CCAAAAAATGG PpuMI 1671 AGGTCCC [GA]G^G[AT]CC[CT] 1999 GGGACCC PshAI 46 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 157 GACTGCAGTC RcaI 54 TCATGA T^CATGA 2784 TCATGA SapI 2124 GAAGAGC GCTCTTC(1/4) 2521 GAAGAGC Van91I 1745 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2964 CCAAAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccI AccII AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BfrI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII