Restriction map for
pFN21AE3454
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI BalI
BbeI BbrPI BbvCI BcgI BclI
BglI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI Bsu36I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FbaI FseI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Asp700I 2394 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3902 TGCGCA TGC^GCA
AvrII 1762 CCTAGG C^CTAGG
BamHI 819 GGATCC G^GATCC
BciVI 3279 GGATAC GTATCC(6/5)
BlnI 1762 CCTAGG C^CTAGG
BlpI 771 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 771 GCTCAGC GC^T[ACGT]AGC
BsaAI 2046 CACGTA [CT]AC^GT[GA]
BsaI 3232 GGTCTC GGTCTC(1/5)
BsmI 1194 GCATTC GAATGC(1/-1)
BspMI 3640 GCAGGT ACCTGC(4/8)
Bst1107I 3223 GTATAC GTA^TAC
BstZ17I 3223 GTATAC GTA^TAC
CelII 771 GCTCAGC GC^T[ACGT]AGC
CfoI 3903 GCGC GCG^C
DraIII 2111 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 825 GACTCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 903 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3902 TGCGCA TGC^GCA
HhaI 3903 GCGC GCG^C
HinP1I 3903 GCGC G^CGC
HindIII 1323 AAGCTT A^AGCTT
MfeI 1756 CAATTG C^AATTG
MspA1I 2562 CAGCTG C[AC]G^C[GT]G
MunI 1756 CAATTG C^AATTG
NruI 3552 TCGCGA TCG^CGA
PvuII 2562 CAGCTG CAG^CTG
SpeI 3238 ACTAGT A^CTAGT
StuI 1747 AGGCCT AGG^CCT
XbaI 2670 TCTAGA T^CTAGA
XcmI 3806 CCATGGACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2394 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 3821 CCGC CCGC(-3/-1)
414 GCGG
BanI 1443 GGTGCC G^G[CT][GA]CC
3652 GGCACC
BcnI 793 CCGGG CC^[GC]GG
1722 CCCGG
BsaWI 1811 ACCGGA [AT]^CCGG[AT]
3314 ACCGGA
BspHI 54 TCATGA T^CATGA
2784 TCATGA
BspLU11I 863 ACATGT A^CATGT
3357 ACATGT
BstXI 2354 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3623 CCAGGCGTTTGG
Cac8I 2647 GCCAGC GC[ACGT]^[ACGT]GC
3389 GCTTGC
DraI 3156 TTTAAA TTT^AAA
3645 TTTAAA
DraII 1671 AGGTCCC [GA]G^G[ACGT]CC[CT]
1999 GGGACCC
DsaI 1053 CCATGG C^C[GA][CT]GG
3806 CCATGG
EaeI 603 TGGCCG [CT]^GGCC[GA]
3818 TGGCCG
EcoO109I 1671 AGGTCCC [GA]G^G[ACGT]CC[CT]
1999 GGGACCC
EcoRV 3556 GATATC GAT^ATC
3745 GATATC
EcoT22I 1690 ATGCAT ATGCA^T
3679 ATGCAT
HincII 1552 GTCAAC GT[CT]^[GA]AC
3361 GTCAAC
HindII 1552 GTCAAC GT[CT]^[GA]AC
3361 GTCAAC
MaeII 2047 ACGT A^CGT
2085 ACGT
NciI 793 CCGGG CC^[GC]GG
1722 CCCGG
NcoI 1053 CCATGG C^CATGG
3806 CCATGG
NdeI 589 CATATG CA^TATG
2164 CATATG
NsiI 1690 ATGCAT ATGCA^T
3679 ATGCAT
PflMI 1745 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2964 CCAAAAAATGG
PpuMI 1671 AGGTCCC [GA]G^G[AT]CC[CT]
1999 GGGACCC
PshAI 46 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
157 GACTGCAGTC
RcaI 54 TCATGA T^CATGA
2784 TCATGA
SapI 2124 GAAGAGC GCTCTTC(1/4)
2521 GAAGAGC
Van91I 1745 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2964 CCAAAAAATGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AccII AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanII BbsI BbvI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cfr13I DdeI
DpnII EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII