Restriction map for
pFN21AE1282
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccI AflII AgeI AhdI
AlwI AocI AscI AseI AsnI
AspEI AvrII BamHI BbrPI BbvCI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI DraI DraIII Eam1105I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI HindIII HpaI MamI
MfeI MflI MluI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI SacI
SalI SapI SbfI ScaI SexAI
SfcI SfuI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp45I
XhoI XhoII
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 895 GGTACC G^GTACC
AclI 2153 AACGTT AA^CGTT
AflIII 1280 ACGTGT A^C[GA][CT]GT
AlwNI 769 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 895 GGTACC G^GTACC
AspI 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 933 GTCTTC GAAGAC(2/6)
BciVI 2163 GGATAC GTATCC(6/5)
BlpI 1062 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1062 GCTGAGC GC^T[ACGT]AGC
BpuAI 933 GTCTTC GAAGAC(2/6)
BsaAI 2015 CACGTA [CT]AC^GT[GA]
CelII 1062 GCTGAGC GC^T[ACGT]AGC
CpoI 1851 CGGTCCG CG^G[AT]CCG
DrdI 939 GACCTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 942 CTCTTC CTCTTC(1/4)
EcoRI 142 GAATTC G^AATTC
HincII 115 GTCAAC GT[CT]^[GA]AC
HindII 115 GTCAAC GT[CT]^[GA]AC
KpnI 895 GGTACC GGTAC^C
Ksp632I 942 CTCTTC CTCTTC(1/4)
NaeI 1980 GCCGGC GCC^GGC
NcoI 2111 CCATGG C^CATGG
NgoMIV 1980 GCCGGC G^CCGGC
NotI 286 GCGGCCGC GC^GGCCGC
NruI 2333 TCGCGA TCG^CGA
PflFI 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2153 AACGTT AA^CGTT
RsrII 1851 CGGTCCG CG^G[AT]CCG
SphI 62 GCATGC GCATG^C
StuI 1628 AGGCCT AGG^CCT
TfiI 2104 GATTC G^A[AT]TC
Tth111I 1210 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2221 TCTAGA T^CTAGA
XcmI 1220 CCAAGCCCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 1635 TCCGGA T^CCGGA
2100 TCCGGA
Alw44I 1930 GTGCAC G^TGCAC
2083 GTGCAC
ApaLI 1930 GTGCAC G^TGCAC
2083 GTGCAC
Asp700I 1245 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1332 GAAGGACTTC
AviII 1076 TGCGCA TGC^GCA
1616 TGCGCA
BalI 1304 TGGCCA TGG^CCA
2114 TGGCCA
BcgI 137 GCATCGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
497 GCAGCCGCTTCG
BsaI 1418 GAGACC GGTCTC(1/5)
2375 GAGACC
BseAI 1635 TCCGGA T^CCGGA
2100 TCCGGA
BseRI 607 GAGGAG GAGGAG(10/8)
1090 GAGGAG
BsgI 1146 CTGCAC GTGCAG(16/14)
2136 CTGCAC
BsmBI 671 CGTCTC CGTCTC(1/5)
1215 CGTCTC
BspEI 1635 TCCGGA T^CCGGA
2100 TCCGGA
BsrBI 201 CCGCTC CCGCTC(-3/-3)
1868 CCGCTC
BssHII 160 GCGCGC G^CGCGC
1661 GCGCGC
BstAPI 768 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2138 GCACCATTTGC
FspI 1076 TGCGCA TGC^GCA
1616 TGCGCA
KspI 105 CCGCGG CCGC^GG
482 CCGCGG
MaeIII 700 GTTAC ^GT[ACGT]AC
2018 GTAAC
MluNI 1304 TGGCCA TGG^CCA
2114 TGGCCA
MroI 1635 TCCGGA T^CCGGA
2100 TCCGGA
MscI 1304 TGGCCA TGG^CCA
2114 TGGCCA
MseI 779 TTAA T^TAA
2357 TTAA
MslI 382 CACGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1875 CATGTCGGTG
NspI 62 GCATGC [GA]CATG^[CT]
1472 ACATGC
PflMI 74 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCACGCCCTGG
PvuII 166 CAGCTG CAG^CTG
1500 CAGCTG
SacII 105 CCGCGG CCGC^GG
482 CCGCGG
SfiI 568 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2238 GGCCCAGGGGGCC
SgrAI 521 CGCCGGTG C[GA]^CCGG[CT]G
2250 CACCGGCG
Tru9I 779 TTAA T^TAA
2357 TTAA
Van91I 74 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCACGCCCTGG
XmnI 1245 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1332 GAAGGACTTC
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Aor51HI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsaWI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco47III Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XmaCI XmaI