Restriction map for
pFN21AE3107
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tth111I Van91I XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 548 GGATC GGATC(4/5)
AlwNI 206 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 109 GGGCCC GGGCC^C
AspHI 35 GAGCAC G[AT]GC[AT]^C
AvaI 67 CTCGAG C^[CT]CG[GA]G
AvaII 317 GGTCC G^G[AT]CC
BanI 244 GGCACC G^G[CT][GA]CC
BbsI 790 GAAGAC GAAGAC(2/6)
BcnI 300 CCGGG CC^[GC]GG
BfaI 655 CTAG C^TAG
BglI 111 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 413 AGATCT A^GATCT
BpuAI 790 GAAGAC GAAGAC(2/6)
BsaAI 196 CACGTA [CT]AC^GT[GA]
BsiHKAI 35 GAGCAC G[AT]GC[AT]^C
BsmAI 603 GAGAC GTCTC(1/5)
BsmI 516 GCATTC GAATGC(1/-1)
BsoBI 67 CTCGAG C^[CT]CG[GA]G
Bsp1407I 431 TGTACA T^GTACA
BspHI 797 TCATGA T^CATGA
BsrDI 138 CATTGC GCAATG(2/0)
BsrGI 431 TGTACA T^GTACA
BstF5I 750 GGATG GGATG(2/0)
BstYI 413 AGATCT [GA]^GATC[CT]
CfoI 105 GCGC GCG^C
DraII 109 GGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 600 GAAGAG CTCTTC(1/4)
EcoO109I 109 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 50 CCATGG C^C[AT][AT]GG
FokI 750 GGATG GGATG(9/13)
HhaI 105 GCGC GCG^C
HinP1I 105 GCGC G^CGC
Ksp632I 600 GAAGAG CTCTTC(1/4)
KspI 5 CCGCGG CCGC^GG
MaeI 655 CTAG C^TAG
MflI 413 AGATCT [GA]^GATC[CT]
NciI 300 CCGGG CC^[GC]GG
NcoI 50 CCATGG C^CATGG
PaeR7I 67 CTCGAG C^TCGAG
RcaI 797 TCATGA T^CATGA
SacII 5 CCGCGG CCGC^GG
SmlI 67 CTCGAG C^T[CT][GA]AG
SspBI 431 TGTACA T^GTACA
StuI 90 AGGCCT AGG^CCT
StyI 50 CCATGG C^C[AT][AT]GG
Tsp509I 639 AATT ^AATT
XbaI 654 TCTAGA T^CTAGA
XhoI 67 CTCGAG C^TCGAG
XhoII 413 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BalI 53 TGGCCA TGG^CCA
669 TGGCCA
BsgI 94 CTGCAC GTGCAG(16/14)
258 CTGCAC
DpnII 414 GATC ^GATC
549 GATC
DsaI 5 CCGCGG C^C[GA][CT]GG
50 CCATGG
HapII 300 CCGG C^CGG
546 CCGG
HinfI 459 GAATC G^A[ACGT]TC
825 GAATC
HpaII 300 CCGG C^CGG
546 CCGG
MaeII 157 ACGT A^CGT
197 ACGT
MboI 414 GATC ^GATC
549 GATC
MboII 600 GAAGA GAAGA(8/7)
790 GAAGA
MluNI 53 TGGCCA TGG^CCA
669 TGGCCA
MscI 53 TGGCCA TGG^CCA
669 TGGCCA
MspI 300 CCGG C^CGG
546 CCGG
NdeII 414 GATC ^GATC
549 GATC
PvuII 364 CAGCTG CAG^CTG
661 CAGCTG
Sau3AI 414 GATC ^GATC
549 GATC
SfcI 203 CTACAG C^T[GA][CT]AG
212 CTGTAG
TfiI 459 GAATC G^A[AT]TC
825 GAATC
Tsp45I 437 GTGAC ^GT[GC]AC
485 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI BstUI BstXI Cac8I Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
HaeIII ItaI MaeIII MnlI MslI
MspA1I MvaI MvnI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI TaqI
TseI TspRI TthHB8I