Restriction map for
pFN21AE3437
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# NO CUT ENZYMES ( 81 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MluI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1996 GACGTC GACGT^C
AccI 1774 GTCTAC GT^[AC][GT]AC
AspI 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 674 TGCGCA TGC^GCA
BamHI 1509 GGATCC G^GATCC
BbrPI 948 CACGTG CAC^GTG
BclI 812 TGATCA T^GATCA
BglI 2121 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1562 AGATCT A^GATCT
BsaBI 2466 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 349 GTGCAG GTGCAG(16/14)
BsmBI 2724 CGTCTC CGTCTC(1/5)
Bsp1407I 2606 TGTACA T^GTACA
BspMI 1778 ACCTGC ACCTGC(4/8)
BsrGI 2606 TGTACA T^GTACA
BstAPI 944 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2435 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2716 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2816 CGGCCG C^GGCCG
EclXI 2816 CGGCCG C^GGCCG
Eco52I 2816 CGGCCG C^GGCCG
EcoRV 1702 GATATC GAT^ATC
FbaI 812 TGATCA T^GATCA
FspI 674 TGCGCA TGC^GCA
MamI 2466 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2097 CAATTG C^AATTG
MunI 2097 CAATTG C^AATTG
NcoI 1505 CCATGG C^CATGG
PflFI 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 948 CACGTG CAC^GTG
PmlI 948 CACGTG CAC^GTG
SapI 2358 GCTCTTC GCTCTTC(1/4)
ScaI 1004 AGTACT AGT^ACT
SmaI 1865 CCCGGG CCC^GGG
SphI 2648 GCATGC GCATG^C
SspBI 2606 TGTACA T^GTACA
Tth111I 2302 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 513 TCTAGA T^CTAGA
XcmI 475 CCAGCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1865 CCCGGG C^CCGGG
XmaI 1865 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 57 TCCGGA T^CCGGA
2598 TCCGGA
ApaI 2005 GGGCCC GGGCC^C
2053 GGGCCC
AspHI 1033 GAGCTC G[AT]GC[AT]^C
2356 GAGCTC
BbvCI 978 CCTCAGC CCTCAGC(-5/-2)
2281 GCTGAGG
BseAI 57 TCCGGA T^CCGGA
2598 TCCGGA
BsiHKAI 1033 GAGCTC G[AT]GC[AT]^C
2356 GAGCTC
BspEI 57 TCCGGA T^CCGGA
2598 TCCGGA
BspHI 869 TCATGA T^CATGA
956 TCATGA
BspLU11I 881 ACATGT A^CATGT
1137 ACATGT
BsrBI 1122 CCGCTC CCGCTC(-3/-3)
688 GAGCGG
DsaI 1374 CCACGG C^C[GA][CT]GG
1505 CCATGG
EcoNI 432 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2379 CCTGGAGAAGG
MroI 57 TCCGGA T^CCGGA
2598 TCCGGA
MseI 1247 TTAA T^TAA
2885 TTAA
NaeI 292 GCCGGC GCC^GGC
2818 GCCGGC
NgoMIV 292 GCCGGC G^CCGGC
2818 GCCGGC
PflMI 1173 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2787 CCAGGACGTGG
PpuMI 1241 AGGACCT [GA]G^G[AT]CC[CT]
2549 GGGACCT
RcaI 869 TCATGA T^CATGA
956 TCATGA
SacI 1033 GAGCTC GAGCT^C
2356 GAGCTC
SexAI 494 ACCTGGT A^CC[AT]GGT
1091 ACCTGGT
StuI 228 AGGCCT AGG^CCT
1079 AGGCCT
Tru9I 1247 TTAA T^TAA
2885 TTAA
Tsp509I 1175 AATT ^AATT
2098 AATT
Van91I 1173 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2787 CCAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII