Restriction map for
pFN21AE4119
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccI AccIII AcsI AfaI
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MslI
MunI NdeI NheI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SbfI ScaI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1120 GACGTC GACGT^C
AclI 1039 AACGTT AA^CGTT
AflII 403 CTTAAG C^TTAAG
AflIII 626 ACACGT A^C[GA][CT]GT
AhdI 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1318 GTGCAC G^TGCAC
AlwNI 1406 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1049 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1303 AGCGCT AGC^GCT
ApaLI 1318 GTGCAC G^TGCAC
AspEI 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 249 CCCGGG C^[CT]CG[GA]G
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BfaI 853 CTAG C^TAG
BfrI 403 CTTAAG C^TTAAG
BsaAI 904 TACGTG [CT]AC^GT[GA]
BseRI 22 GAGGAG GAGGAG(10/8)
BsiEI 261 CGGCCG CG[GA][CT]^CG
BsmBI 601 GAGACG CGTCTC(1/5)
BsoBI 249 CCCGGG C^[CT]CG[GA]G
BsrFI 227 GCCGGC [GA]^CCGG[CT]
BssSI 421 CTCGTG CACGAG(-5/-1)
BstYI 749 AGATCC [GA]^GATC[CT]
Bsu36I 1049 CCTCAGG CC^T[ACGT]AGG
Cfr10I 227 GCCGGC [GA]^CCGG[CT]
EagI 261 CGGCCG C^GGCCG
Eam1105I 61 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 261 CGGCCG C^GGCCG
Eco47III 1303 AGCGCT AGC^GCT
Eco52I 261 CGGCCG C^GGCCG
Eco81I 1049 CCTCAGG CC^T[ACGT]AGG
EcoT14I 356 CCATGG C^C[AT][AT]GG
EcoT22I 445 ATGCAT ATGCA^T
FauI 172 CCCGC CCCGC(4/6)
HgaI 219 GACGC GACGC(5/10)
KspI 96 CCGCGG CCGC^GG
MaeI 853 CTAG C^TAG
MaeIII 1408 GTGAC ^GT[ACGT]AC
MflI 749 AGATCC [GA]^GATC[CT]
NaeI 227 GCCGGC GCC^GGC
NcoI 356 CCATGG C^CATGG
NgoMIV 227 GCCGGC G^CCGGC
NotI 260 GCGGCCGC GC^GGCCGC
NsiI 445 ATGCAT ATGCA^T
Psp1406I 1039 AACGTT AA^CGTT
SacII 96 CCGCGG CCGC^GG
SfcI 1299 CTACAG C^T[GA][CT]AG
SfiI 253 GGCCTGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 226 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 249 CCCGGG CCC^GGG
SmlI 403 CTTAAG C^T[CT][GA]AG
StuI 374 AGGCCT AGG^CCT
StyI 356 CCATGG C^C[AT][AT]GG
Tsp45I 1408 GTGAC ^GT[GC]AC
Tsp509I 1131 AATT ^AATT
XcmI 188 CCAACTGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 749 AGATCC [GA]^GATC[CT]
XmaCI 249 CCCGGG C^CCGGG
XmaI 249 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 97 CGCG CG^CG
177 CGCG
AlwI 750 GATCC GGATC(4/5)
1272 GGATC
ApaI 194 GGGCCC GGGCC^C
237 GGGCCC
AspHI 340 GAGCAC G[AT]GC[AT]^C
1318 GTGCAC
AvaII 60 GGACC G^G[AT]CC
564 GGACC
BanII 194 GGGCCC G[GA]GC[CT]^C
237 GGGCCC
BglI 254 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
276 GCCGCTGCGGC
BpmI 706 CTGGAG CTGGAG(16/14)
1090 CTGGAG
BsiHKAI 340 GAGCAC G[AT]GC[AT]^C
1318 GTGCAC
BspMI 31 ACCTGC ACCTGC(4/8)
1322 ACCTGC
BstUI 97 CGCG CG^CG
177 CGCG
BstXI 38 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1363 CCATTTTTTTGG
DraIII 477 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1252 CACCGAGTG
DsaI 96 CCGCGG C^C[GA][CT]GG
356 CCATGG
HincII 27 GTTGAC GT[CT]^[GA]AC
735 GTTGAC
HindII 27 GTTGAC GT[CT]^[GA]AC
735 GTTGAC
HphI 786 GGTGA GGTGA(8/7)
1046 TCACC
MseI 404 TTAA T^TAA
631 TTAA
MvnI 97 CGCG CG^CG
177 CGCG
PpuMI 59 AGGACCC [GA]G^G[AT]CC[CT]
563 AGGACCT
SapI 687 GAAGAGC GCTCTTC(1/4)
285 GCTCTTC
Tru9I 404 TTAA T^TAA
631 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcyI AluI BalI BanI
BbeI BbsI BbvI BcnI BmyI
BpuAI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
Ksp632I MaeII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI Sau3AI Sau96I
ScrFI SfaNI SfoI TaqI TfiI
TseI TspRI TthHB8I Van91I