Restriction map for
pFN21AB6260
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BamHI BbeI BbrPI BcgI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1312 CGCG CG^CG
AccIII 459 TCCGGA T^CCGGA
AcyI 910 GACGCC G[GA]^CG[CT]C
AhdI 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1771 GTGCAC G^TGCAC
ApaLI 1771 GTGCAC G^TGCAC
AspEI 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 814 TGCGCA TGC^GCA
BalI 827 TGGCCA TGG^CCA
BanI 1536 GGCACC G^G[CT][GA]CC
BbvCI 1351 GCTGAGG CCTCAGC(-5/-2)
BclI 930 TGATCA T^GATCA
BglII 1358 AGATCT A^GATCT
BsaHI 910 GACGCC G[GA]^CG[CT]C
BsaWI 459 TCCGGA [AT]^CCGG[AT]
BseAI 459 TCCGGA T^CCGGA
BspEI 459 TCCGGA T^CCGGA
BspHI 665 TCATGA T^CATGA
BspMI 1658 ACCTGC ACCTGC(4/8)
BsrBI 1171 GAGCGG CCGCTC(-3/-3)
BsrI 161 ACTGG ACTGG(1/-1)
BssSI 625 CACGAG CACGAG(-5/-1)
BstEII 1609 GGTTACC G^GT[ACGT]ACC
BstPI 1609 GGTTACC G^GT[ACGT]ACC
BstUI 1312 CGCG CG^CG
BstXI 23 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1358 AGATCT [GA]^GATC[CT]
DraI 178 TTTAAA TTT^AAA
DsaI 1430 CCATGG C^C[GA][CT]GG
Eam1105I 58 GACCGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1609 GGTTACC G^GT[ACGT]ACC
EcoT22I 96 ATGCAT ATGCA^T
FbaI 930 TGATCA T^GATCA
FspI 814 TGCGCA TGC^GCA
HaeII 1272 GGCGCT [GA]GCGC^[CT]
HgaI 910 GACGC GACGC(5/10)
Hin1I 910 GACGCC G[GA]^CG[CT]C
MaeIII 1610 GTTAC ^GT[ACGT]AC
MflI 1358 AGATCT [GA]^GATC[CT]
MluNI 827 TGGCCA TGG^CCA
MroI 459 TCCGGA T^CCGGA
MscI 827 TGGCCA TGG^CCA
MslI 1431 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1312 CGCG CG^CG
NcoI 1430 CCATGG C^CATGG
NsiI 96 ATGCAT ATGCA^T
PaeR7I 973 CTCGAG C^TCGAG
RcaI 665 TCATGA T^CATGA
StuI 1418 AGGCCT AGG^CCT
XhoI 973 CTCGAG C^TCGAG
XhoII 1358 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1554 AAATTC [GA]^AATT[CT]
1573 AAATTC
AflIII 197 ACATGT A^C[GA][CT]GT
282 ACATGT
AlwI 264 GATCC GGATC(4/5)
543 GGATC
AocI 985 CCTGAGG CC^T[ACGT]AGG
2150 CCTCAGG
ApoI 1554 AAATTC [GA]^AATT[CT]
1573 AAATTC
BanII 820 GAGCTC G[GA]GC[CT]^C
1180 GAGCTC
BcnI 1300 CCCGG CC^[GC]GG
1410 CCGGG
BglI 1107 GCCCAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1261 GCCCGAGAGGC
BsgI 870 GTGCAG GTGCAG(16/14)
1814 GTGCAG
BsmI 94 GAATGC GAATGC(1/-1)
564 GAATGC
BspLU11I 197 ACATGT A^CATGT
282 ACATGT
Bsu36I 985 CCTGAGG CC^T[ACGT]AGG
2150 CCTCAGG
Eco81I 985 CCTGAGG CC^T[ACGT]AGG
2150 CCTCAGG
EcoT14I 737 CCAAGG C^C[AT][AT]GG
1430 CCATGG
MaeII 842 ACGT A^CGT
1845 ACGT
NciI 1300 CCCGG CC^[GC]GG
1410 CCGGG
PstI 1162 CTGCAG CTGCA^G
1900 CTGCAG
SacI 820 GAGCTC GAGCT^C
1180 GAGCTC
SfcI 1162 CTGCAG C^T[GA][CT]AG
1900 CTGCAG
StyI 737 CCAAGG C^C[AT][AT]GG
1430 CCATGG
TspRI 705 CAGTG CA[GC]TG[ACGT][ACGT]^
880 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BbsI BbvI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I