Restriction map for
pFN21AE2021
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
AlwNI AscI AseI AsnI Asp700I
Asp718I AviII BalI BanI BbeI
BcgI BciVI BlpI Bpu1102I BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrBI BssHII BstAPI
BstBI BstEII BstPI BstXI CelII
ClaI CpoI DraI DraIII DrdI
EarI EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI Ksp632I MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 73 enzymes) #
###################################
AccI 297 GTATAC GT^[AC][GT]AC
AclI 1294 AACGTT AA^CGTT
AcsI 363 AAATTC [GA]^AATT[CT]
AcyI 13 GACGCC G[GA]^CG[CT]C
AflII 1132 CTTAAG C^TTAAG
AhdI 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 736 GTGCAC G^TGCAC
AocI 36 CCTAAGG CC^T[ACGT]AGG
Aor51HI 324 AGCGCT AGC^GCT
ApaI 1070 GGGCCC GGGCC^C
ApaLI 736 GTGCAC G^TGCAC
ApoI 363 AAATTC [GA]^AATT[CT]
AspEI 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 736 GTGCAC G[AT]GC[AT]^C
AspI 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1067 CCCGGG C^[CT]CG[GA]G
AvrII 1542 CCTAGG C^CTAGG
BanII 1070 GGGCCC G[GA]GC[CT]^C
BbrPI 118 CACGTG CAC^GTG
BclI 1005 TGATCA T^GATCA
BfrI 1132 CTTAAG C^TTAAG
BglI 1759 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 834 AGATCT A^GATCT
BlnI 1542 CCTAGG C^CTAGG
BsaAI 118 CACGTG [CT]AC^GT[GA]
BsaBI 901 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 348 GAGACC GGTCTC(1/5)
BsgI 145 CTGCAC GTGCAG(16/14)
BsiEI 134 CGGCCG CG[GA][CT]^CG
BsiHKAI 736 GTGCAC G[AT]GC[AT]^C
BsmI 282 GAATGC GAATGC(1/-1)
BsoBI 1067 CCCGGG C^[CT]CG[GA]G
Bsp1407I 533 TGTACA T^GTACA
BspHI 264 TCATGA T^CATGA
BsrFI 50 ACCGGC [GA]^CCGG[CT]
BsrGI 533 TGTACA T^GTACA
BssSI 947 CACGAG CACGAG(-5/-1)
Bst1107I 297 GTATAC GTA^TAC
BstZ17I 297 GTATAC GTA^TAC
Bsu36I 36 CCTAAGG CC^T[ACGT]AGG
Cfr10I 50 ACCGGC [GA]^CCGG[CT]
EaeI 134 CGGCCG [CT]^GGCC[GA]
EagI 134 CGGCCG C^GGCCG
Eam1105I 1624 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 134 CGGCCG C^GGCCG
Eco47III 324 AGCGCT AGC^GCT
Eco52I 134 CGGCCG C^GGCCG
Eco81I 36 CCTAAGG CC^T[ACGT]AGG
FbaI 1005 TGATCA T^GATCA
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HindIII 825 AAGCTT A^AGCTT
MamI 901 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 302 CCATGG C^CATGG
NotI 133 GCGGCCGC GC^GGCCGC
PflFI 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 231 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 118 CACGTG CAC^GTG
PmlI 118 CACGTG CAC^GTG
PpuMI 1730 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1294 AACGTT AA^CGTT
PvuII 1099 CAGCTG CAG^CTG
RcaI 264 TCATGA T^CATGA
SfcI 1050 CTATAG C^T[GA][CT]AG
SmaI 1067 CCCGGG CCC^GGG
SspBI 533 TGTACA T^GTACA
TfiI 253 GATTC G^A[AT]TC
Tth111I 1522 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 231 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1635 TCTAGA T^CTAGA
XmaCI 1067 CCCGGG C^CCGGG
XmaI 1067 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 84 CGCG CG^CG
138 CGCG
BpmI 851 CTGGAG CTGGAG(16/14)
1735 CTGGAG
BstNI 1734 CCTGG CC^[AT]GG
1773 CCAGG
BstUI 84 CGCG CG^CG
138 CGCG
CfoI 325 GCGC GCG^C
555 GCGC
DraII 1070 GGGCCCT [GA]G^G[ACGT]CC[CT]
1730 GGGACCT
EcoO109I 1070 GGGCCCT [GA]G^G[ACGT]CC[CT]
1730 GGGACCT
EcoRII 1734 CCTGG ^CC[AT]GG
1773 CCAGG
HaeII 324 AGCGCT [GA]GCGC^[CT]
554 AGCGCC
HhaI 325 GCGC GCG^C
555 GCGC
HinP1I 325 GCGC G^CGC
555 GCGC
KspI 83 CCGCGG CCGC^GG
137 CCGCGG
MseI 1133 TTAA T^TAA
1427 TTAA
MslI 663 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
739 CACACTGGTG
MvaI 1734 CCTGG CC^[AT]GG
1773 CCAGG
MvnI 84 CGCG CG^CG
138 CGCG
NspI 1011 GCATGC [GA]CATG^[CT]
1790 GCATGC
SacII 83 CCGCGG CCGC^GG
137 CCGCGG
SphI 1011 GCATGC GCATG^C
1790 GCATGC
Tru9I 1133 TTAA T^TAA
1427 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BbsI BbvCI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII