Restriction map for
pFN21AE0999
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BanII
BbeI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI NarI NcoI NheI
NotI NruI NsiI NspV PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 856 CGCG CG^CG
AcsI 844 AAATTT [GA]^AATT[CT]
AfaI 1287 GTAC GT^AC
AflIII 468 ACGTGT A^C[GA][CT]GT
Alw44I 859 GTGCAC G^TGCAC
AlwNI 578 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 859 GTGCAC G^TGCAC
ApoI 844 AAATTT [GA]^AATT[CT]
AspI 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1329 GGACC G^G[AT]CC
BalI 241 TGGCCA TGG^CCA
BamHI 1534 GGATCC G^GATCC
BanI 1484 GGCACC G^G[CT][GA]CC
BbrPI 467 CACGTG CAC^GTG
BbvCI 1417 GCTGAGG CCTCAGC(-5/-2)
BcgI 536 CGAGGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 918 TGATCA T^GATCA
BcnI 1509 CCGGG CC^[GC]GG
BglII 768 AGATCT A^GATCT
BsaAI 467 CACGTG [CT]AC^GT[GA]
BsaI 1433 GAGACC GGTCTC(1/5)
BseRI 596 GAGGAG GAGGAG(10/8)
Bsp1407I 1286 TGTACA T^GTACA
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BsrGI 1286 TGTACA T^GTACA
BstAPI 1485 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 856 CGCG CG^CG
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
Cfr13I 1329 GGACC G^G[ACGT]CC
EarI 331 GAAGAG CTCTTC(1/4)
FbaI 918 TGATCA T^GATCA
HincII 655 GTTGAC GT[CT]^[GA]AC
HindII 655 GTTGAC GT[CT]^[GA]AC
HindIII 761 AAGCTT A^AGCTT
Ksp632I 331 GAAGAG CTCTTC(1/4)
MluNI 241 TGGCCA TGG^CCA
MscI 241 TGGCCA TGG^CCA
MvnI 856 CGCG CG^CG
NaeI 76 GCCGGC GCC^GGC
NciI 1509 CCGGG CC^[GC]GG
NdeI 914 CATATG CA^TATG
NgoMIV 76 GCCGGC G^CCGGC
NspI 462 ACATGC [GA]CATG^[CT]
PacI 454 TTAATTAA TTAAT^TAA
PflFI 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1043 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 658 GACTC GAGTC(4/5)
PmaCI 467 CACGTG CAC^GTG
PmlI 467 CACGTG CAC^GTG
RsaI 1287 GTAC GT^AC
Sau96I 1329 GGACC G^G[ACGT]CC
SexAI 1331 ACCAGGT A^CC[AT]GGT
SfcI 908 CTGTAG C^T[GA][CT]AG
SspBI 1286 TGTACA T^GTACA
TfiI 999 GATTC G^A[AT]TC
Tth111I 1330 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1043 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 973 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 892 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
994 GAAGTGATTC
AvaI 102 CTCGGG C^[CT]CG[GA]G
116 CTCGGG
BbsI 524 GAAGAC GAAGAC(2/6)
223 GTCTTC
BbvI 128 GCAGC GCAGC(8/12)
143 GCAGC
BfaI 742 CTAG C^TAG
974 CTAG
BpuAI 524 GAAGAC GAAGAC(2/6)
223 GTCTTC
BsmFI 119 GGGAC GGGAC(10/14)
1215 GTCCC
BsoBI 102 CTCGGG C^[CT]CG[GA]G
116 CTCGGG
BspMI 530 ACCTGC ACCTGC(4/8)
546 GCAGGT
BsrDI 1347 CATTGC GCAATG(2/0)
1490 CATTGC
DraI 441 TTTAAA TTT^AAA
873 TTTAAA
FauI 506 CCCGC CCCGC(4/6)
854 CCCGC
Fnu4HI 128 GCAGC GC^[ACGT]GC
143 GCAGC
HapII 77 CCGG C^CGG
1509 CCGG
HinfI 658 GACTC G^A[ACGT]TC
999 GATTC
HpaII 77 CCGG C^CGG
1509 CCGG
HphI 1093 GGTGA GGTGA(8/7)
59 TCACC
ItaI 128 GCAGC GC^[ACGT]GC
143 GCAGC
MaeI 742 CTAG C^TAG
974 CTAG
MspI 77 CCGG C^CGG
1509 CCGG
NlaIV 1484 GGCACC GG[ACGT]^[ACGT]CC
1534 GGATCC
SfaNI 1414 GATGC GCATC(5/9)
107 GCATC
TaqI 1233 TCGA T^CGA
1464 TCGA
TseI 128 GCAGC G^C[AT]GC
143 GCAGC
Tsp45I 58 GTCAC ^GT[GC]AC
265 GTGAC
TthHB8I 1233 TCGA T^CGA
1464 TCGA
XcmI 1346 CCATTGCGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1471 CCAATAACGCACTGG
XmnI 892 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
994 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AspHI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EaeI EcoRII FokI HaeIII
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI Tru9I
Tsp509I TspRI XhoII