Restriction map for
pFN21AE0998
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# NO CUT ENZYMES ( 77 enzymes) #
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Acc65I AclI AflIII AgeI AhdI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BcgI BclI BsaAI BsaBI BsiWI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco81I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SfiI SfuI
SnaBI SpeI SspI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1129 GACGTC GACGT^C
AccIII 3167 TCCGGA T^CCGGA
AcsI 2847 AAATTC [GA]^AATT[CT]
AflII 2653 CTTAAG C^TTAAG
Alw44I 30 GTGCAC G^TGCAC
Aor51HI 1760 AGCGCT AGC^GCT
ApaLI 30 GTGCAC G^TGCAC
ApoI 2847 AAATTC [GA]^AATT[CT]
AviII 3029 TGCGCA TGC^GCA
AvrII 2967 CCTAGG C^CTAGG
BciVI 681 GGATAC GTATCC(6/5)
BfrI 2653 CTTAAG C^TTAAG
BglII 2312 AGATCT A^GATCT
BlnI 2967 CCTAGG C^CTAGG
BlpI 111 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BseAI 3167 TCCGGA T^CCGGA
BsiEI 3221 CGGCCG CG[GA][CT]^CG
BsmI 3109 GAATGC GAATGC(1/-1)
BspEI 3167 TCCGGA T^CCGGA
BstAPI 23 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2694 GGTCACC G^GT[ACGT]ACC
BstPI 2694 GGTCACC G^GT[ACGT]ACC
CelII 111 GCTGAGC GC^T[ACGT]AGC
DrdI 1600 GACTGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3221 CGGCCG C^GGCCG
EclXI 3221 CGGCCG C^GGCCG
Eco47III 1760 AGCGCT AGC^GCT
Eco52I 3221 CGGCCG C^GGCCG
EcoO65I 2694 GGTCACC G^GT[ACGT]ACC
FseI 761 GGCCGGCC GGCCGG^CC
FspI 3029 TGCGCA TGC^GCA
MroI 3167 TCCGGA T^CCGGA
PshAI 2782 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 43 CCTGCAGG CCTGCA^GG
SexAI 911 ACCTGGT A^CC[AT]GGT
SgrAI 2748 CACCGGCG C[GA]^CCGG[CT]G
SphI 1658 GCATGC GCATG^C
Sse8387I 43 CCTGCAGG CCTGCA^GG
StuI 509 AGGCCT AGG^CCT
Tsp509I 2848 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 361 GTCTAC GT^[AC][GT]AC
1609 GTCTAC
BsaI 856 GAGACC GGTCTC(1/5)
3229 GAGACC
BsaWI 3167 TCCGGA [AT]^CCGG[AT]
3261 TCCGGT
BsmBI 1326 CGTCTC CGTCTC(1/5)
249 GAGACG
Bsp1407I 1682 TGTACA T^GTACA
1979 TGTACA
BsrGI 1682 TGTACA T^GTACA
1979 TGTACA
BssHII 2102 GCGCGC G^CGCGC
3116 GCGCGC
DraIII 24 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3094 CACTGCGTG
EcoNI 2967 CCTAGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3075 CCTCCACAAGG
HincII 1287 GTCAAC GT[CT]^[GA]AC
2176 GTTGAC
HindII 1287 GTCAAC GT[CT]^[GA]AC
2176 GTTGAC
MseI 1745 TTAA T^TAA
2654 TTAA
NcoI 59 CCATGG C^CATGG
3242 CCATGG
PflMI 120 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3093 CCACTGCGTGG
SapI 1638 GAAGAGC GCTCTTC(1/4)
3120 GCTCTTC
SmlI 736 CTCAAG C^T[CT][GA]AG
2653 CTTAAG
SspBI 1682 TGTACA T^GTACA
1979 TGTACA
Tru9I 1745 TTAA T^TAA
2654 TTAA
Van91I 120 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3093 CCACTGCGTGG
XbaI 2790 TCTAGA T^CTAGA
3444 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI