Restriction map for
pFN21AB6536
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvI
BcgI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FauI Fnu4HI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeII MamI
MluI MroI MslI MspA1I MspI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI TseI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 689 GTAGAC GT^[AC][GT]AC
AcsI 544 GAATTC [GA]^AATT[CT]
AflIII 873 ACATGT A^C[GA][CT]GT
AlwI 552 GATCC GGATC(4/5)
Aor51HI 1088 AGCGCT AGC^GCT
ApoI 544 GAATTC [GA]^AATT[CT]
AvaII 1000 GGTCC G^G[AT]CC
BanI 626 GGTGCC G^G[CT][GA]CC
BanII 947 GGGCTC G[GA]GC[CT]^C
BbvCI 989 CCTCAGC CCTCAGC(-5/-2)
BciVI 1071 GTATCC GTATCC(6/5)
BclI 612 TGATCA T^GATCA
BglII 706 AGATCT A^GATCT
BmyI 947 GGGCTC G[AGT]GC[ACT]^C
BseRI 759 GAGGAG GAGGAG(10/8)
Bsp1286I 947 GGGCTC G[AGT]GC[ACT]^C
BspDI 332 ATCGAT AT^CGAT
BspLU11I 873 ACATGT A^CATGT
BssSI 41 CTCGTG CACGAG(-5/-1)
BstBI 232 TTCGAA TT^CGAA
BstXI 905 CCAAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 706 AGATCT [GA]^GATC[CT]
Cac8I 423 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 1089 GCGC GCG^C
ClaI 332 ATCGAT AT^CGAT
DraII 999 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1088 AGCGCT AGC^GCT
EcoO109I 999 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 544 GAATTC G^AATTC
FbaI 612 TGATCA T^GATCA
HaeII 1088 AGCGCT [GA]GCGC^[CT]
HhaI 1089 GCGC GCG^C
HinP1I 1089 GCGC G^CGC
HphI 861 TCACC GGTGA(8/7)
MflI 706 AGATCT [GA]^GATC[CT]
MseI 164 TTAA T^TAA
NspI 873 ACATGT [GA]CATG^[CT]
NspV 232 TTCGAA TT^CGAA
PleI 463 GAGTC GAGTC(4/5)
PpuMI 999 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 648 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 856 GCTCTTC GCTCTTC(1/4)
SfcI 851 CTGTAG C^T[GA][CT]AG
SfuI 232 TTCGAA TT^CGAA
SmlI 786 CTCAAG C^T[CT][GA]AG
Tru9I 164 TTAA T^TAA
XcmI 630 CCAAAGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 706 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 296 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
845 CAGATTCTG
BalI 170 TGGCCA TGG^CCA
914 TGGCCA
BfaI 91 CTAG C^TAG
454 CTAG
Bsp1407I 212 TGTACA T^GTACA
292 TGTACA
BsrGI 212 TGTACA T^GTACA
292 TGTACA
Cfr13I 442 GGGCC G^G[ACGT]CC
1000 GGTCC
EarI 657 CTCTTC CTCTTC(1/4)
857 CTCTTC
Ksp632I 657 CTCTTC CTCTTC(1/4)
857 CTCTTC
MaeI 91 CTAG C^TAG
454 CTAG
MfeI 812 CAATTG C^AATTG
968 CAATTG
MluNI 170 TGGCCA TGG^CCA
914 TGGCCA
MscI 170 TGGCCA TGG^CCA
914 TGGCCA
MunI 812 CAATTG C^AATTG
968 CAATTG
Sau96I 442 GGGCC G^G[ACGT]CC
1000 GGTCC
SspBI 212 TGTACA T^GTACA
292 TGTACA
SspI 586 AATATT AAT^ATT
1113 AATATT
TfiI 450 GATTC G^A[AT]TC
847 GATTC
Tsp45I 909 GTCAC ^GT[GC]AC
1082 GTCAC
TspRI 911 CACTG CA[GC]TG[ACGT][ACGT]^
1039 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI AluI BsmAI BsrI BssKI
BstF5I BstNI DdeI DpnII EaeI
EcoRII FokI HaeIII HinfI MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
SfaNI TaqI Tsp509I TthHB8I