Restriction map for
pFN21AE1995
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoO65I EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeIII MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AcsI 885 GAATTC [GA]^AATT[CT]
AlwI 1553 GATCC GGATC(4/5)
ApoI 885 GAATTC [GA]^AATT[CT]
BbsI 1059 GAAGAC GAAGAC(2/6)
BglII 1571 AGATCT A^GATCT
BpuAI 1059 GAAGAC GAAGAC(2/6)
BsaWI 842 ACCGGA [AT]^CCGG[AT]
BsgI 1276 GTGCAG GTGCAG(16/14)
BssHII 174 GCGCGC G^CGCGC
BstXI 1376 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1571 AGATCT [GA]^GATC[CT]
DraIII 1579 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 342 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 885 GAATTC G^AATTC
EcoT22I 949 ATGCAT ATGCA^T
MflI 1571 AGATCT [GA]^GATC[CT]
MseI 1013 TTAA T^TAA
NcoI 629 CCATGG C^CATGG
NotI 381 GCGGCCGC GC^GGCCGC
NsiI 949 ATGCAT ATGCA^T
NspI 1307 ACATGC [GA]CATG^[CT]
PaeR7I 1342 CTCGAG C^TCGAG
SapI 714 GCTCTTC GCTCTTC(1/4)
ScaI 131 AGTACT AGT^ACT
StuI 623 AGGCCT AGG^CCT
Tru9I 1013 TTAA T^TAA
Tsp509I 886 AATT ^AATT
XhoI 1342 CTCGAG C^TCGAG
XhoII 1571 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 132 GTAC GT^AC
1713 GTAC
Alw44I 786 GTGCAC G^TGCAC
912 GTGCAC
AlwNI 877 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1618 CAGCACCTG
Aor51HI 215 AGCGCT AGC^GCT
1325 AGCGCT
ApaLI 786 GTGCAC G^TGCAC
912 GTGCAC
BalI 533 TGGCCA TGG^CCA
1604 TGGCCA
BbeI 249 GGCGCC GGCGC^C
1351 GGCGCC
BbvCI 44 CCTCAGC CCTCAGC(-5/-2)
959 CCTCAGC
BfaI 967 CTAG C^TAG
974 CTAG
BsaAI 1201 TACGTG [CT]AC^GT[GA]
1381 TACGTG
BsaI 511 GAGACC GGTCTC(1/5)
1540 GGTCTC
BseRI 24 CTCCTC GAGGAG(10/8)
364 GAGGAG
BspMI 1238 ACCTGC ACCTGC(4/8)
1691 ACCTGC
BsrBI 444 CCGCTC CCGCTC(-3/-3)
1258 GAGCGG
DpnII 1553 GATC ^GATC
1572 GATC
EagI 382 CGGCCG C^GGCCG
449 CGGCCG
EclXI 382 CGGCCG C^GGCCG
449 CGGCCG
Eco47III 215 AGCGCT AGC^GCT
1325 AGCGCT
Eco52I 382 CGGCCG C^GGCCG
449 CGGCCG
HgaI 1185 GACGC GACGC(5/10)
304 GCGTC
KasI 249 GGCGCC G^GCGCC
1351 GGCGCC
KspI 67 CCGCGG CCGC^GG
379 CCGCGG
MaeI 967 CTAG C^TAG
974 CTAG
MboI 1553 GATC ^GATC
1572 GATC
MluNI 533 TGGCCA TGG^CCA
1604 TGGCCA
MscI 533 TGGCCA TGG^CCA
1604 TGGCCA
MslI 819 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1340 CACTCGAGTG
NarI 249 GGCGCC GG^CGCC
1351 GGCGCC
NdeII 1553 GATC ^GATC
1572 GATC
RsaI 132 GTAC GT^AC
1713 GTAC
SacI 712 GAGCTC GAGCT^C
901 GAGCTC
SacII 67 CCGCGG CCGC^GG
379 CCGCGG
Sau3AI 1553 GATC ^GATC
1572 GATC
SexAI 1404 ACCAGGT A^CC[AT]GGT
1580 ACCTGGT
SfoI 249 GGCGCC GGC^GCC
1351 GGCGCC
SmlI 796 CTTGAG C^T[CT][GA]AG
1342 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PstI PvuII Sau96I ScrFI
SfaNI SfcI SfiI SmaI StyI
TaqI TfiI TseI TspRI TthHB8I
XmaCI XmaI